Environmental Engineering / Çevre Mühendisliği

Permanent URI for this collectionhttps://hdl.handle.net/11147/4321

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Author: search.filters.author.Çelik, Ekrem ŞanverInstitution Author: search.filters.institutionAuthor.Küçüker, Mehmet Ali

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  • Article
    Cyanobacterial Communities in Mucilage Collected From Çanakkale Strait (dardanelles): Metagenomic Approach
    (Çanakkale Onsekiz Mart Üniversitesi, 2022) Yılmaz, Sevdan; Küçüker, Mehmet Ali; Kahraman Yılmaz, Dilek; Çelik, Ekrem Şanver; Küçüker, Mehmet Ali; 03.07. Department of Environmental Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    In this study, cyanobacterial communities in mucilage samples collected from three stations in the Dardanelles Strait were analyzed with a metagenomic approach. Mucilage samples were collected at the beginning of June 2021 from three points of the Dardanelles (Station 1: 40°6'42.78"N, 26°23'57.00"E; Station 2: 40°9'8.09"N, 26°24'16.19"E; Station 3: 40°6 '21.62"N, 26°22'41.25"E). The dominant cyanobacteria were Prochlorococcus marinus (39.17%), Synechococcus sp. (20.85%), Lyngbya sp. (12.00%), Trichodesmium erythraeum (7.33%), Aphanocapsa sp. (4.33%) and Leptolyngbya sp. (3.33%), which constituted 87.00 % of the total number of sequences. In this study, cyanobacteria species that can cause harmful algal blooms and have toxic effects on the mucilage structure have been determined. The Marmara Sea and the Dardanelles Strait, which have been affected by serious disturbances, including industrial activities, anthropogenic impacts, tourism and artificial lighting, will never be fully restored to their former ecological state. In addition, cyanobacteria species in the mucilage may cause harmful algal blooms and have toxic effects that threaten the future well-being of coastal populations and ecosystem stability. Thus, the government and local authorities should pay more attention to combating the mucilage.
  • Article
    Shotgun Metagenomic Analysis for Mucilage in the Surface Waters of the Çanakkale Strait (dardanelles): Metabolic Diversity, Microbial Community Structure and Antibiotic Resistance Genes
    (2021) Yılmaz, Sevdan; Küçüker, Mehmet Ali; Kahraman, Dilek; Çelik, Ekrem Şanver; Küçüker, Mehmet Ali; 03.07. Department of Environmental Engineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    In this study, we used shotgun metagenome sequencing to examine the metabolic diversity, microbial community structure and diverse antimicrobial resistance genes of mucilage in the surface waters of the Çanakkale Strait (Dardanelles). Mucilage samples were collected in April 2021 from the three different stations of the Dardanelles. The dominant microbial communities at the phylum level were Bacteroidetes (20.06%), Proteobacteria (13.68%), Verrucomicrobia (6.25%), Planctomycetes (3.02%) and Cyanobacteria (2.5%). Metabolic pathway analysis using KEGG (Kyoto Encyclopedia of Genes and Genomes) revealed that most of the genes of mucilage samples were involved in unclassified (73.86%) followed by metabolism (14.45%), genetic information processing (4.16%), environmental information processing (2.57%), cellular processing (1.88%), human diseases (1.61%), and organismal systems (1.47%). The dfrA3 gene was the most prevalent (20.36%) followed by CRP (18.17%), PmrE (14.92%), rpoB2 (11.17%), SoxR (7.49%), AbeS (6.83%), baeR (5.22%), PmrF (3.70%), dfrA22 (2.20%), dfrA26 (1.76%), dfrA20 (1.63%), golS (1.26%), CAT (1.03%), mtrA (1.01%), TMB-1 (0.64%), novA (0.64%), dfrK (0.59%), vanXB (0.48%), dfrG (0.39%), FosC2 (0.31%), and MexA (0.20%) genes. Antibiotic resistance gene (ARG) types mainly included the resistance genes of multidrug (40.19%), trimethoprim (26.93%), polymyxin (18.62%), rifamycin (11.17%), chloramphenicol (1.03%), aminocoumarin (0.64%), beta-lactamase (0.64%), fosfomycin (0.31%), and vancomycin (0.48%). Antibiotic-resistant bacteria in mucilage can adhere to human skin during swimming, fishing, water sports etc., enter the body through the nose and mouth, and transfer genetic information to the bacteria in contact areas in the human body. Therefore, this situation is risky in public health, and necessary precautions should be taken. In the light of these findings, it has been observed that there is a need for more detailed studies in the future.