IZTECH Research Centers Collection / İYTE Araştırma Merkezleri Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/11147/2636
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Article Citation - WoS: 1Citation - Scopus: 1Triploid Induction of Green Tiger Shrimp, Penaeus Semisulcatus (de Haan, 1844) Using Temperature and Chemical Shock(John Wiley and Sons Inc., 2015) Kır, Mehmet; Tarhan, Gökhan; Okur, ÖzgürTriploidy in fertilized eggs of Penaeus semisulcatus was induced by temperature and chemical shocks. The eggs, which were obtained from the shrimp broodstock maintained at 29C, were exposed to cold temperature (8, 10, 12, and 14 C) and 6-dimetiloaminopurine (6-DMAP) concentrations (100, 150, 200, and 250 μM) for different durations (4, 6, and 8min) 9min after spawning was detected. While the highest triploidy rate of 49.7±4.5% was obtained with a 200μM 6-DMAP concentration for a duration of 8min, the best mean triploidy rate of 45.5±2.8% for cold shock was obtained at a temperature of 10 C for a duration of 8min. Temperature and 6-DMAP concentration did not have significant effect on triploidy rate (P>0.05) but shock duration had significant effect on triploidy rate for individual cold temperature shock or 6-DMAP chemical shock (P<0.05). Although longer durations of shock agent increased the rates of triploid induction, they generally had an adverse effect on hatching rates in the study.Article Citation - WoS: 1Citation - Scopus: 2Induction of Triploidy in Melicertus Kerathurus (forskal, 1775) With Temperature Shock(John Wiley and Sons Inc., 2016) Kır, Mehmet; Şahan, Ali Kemal; Okur, ÖzgürTriploidy in fertilized eggs of Melicertus kerathurus was induced by cold (8, 10, 12°C) and heat (34, 36, 38°C) shock for different duration times (2, 4 and 8 min) after 10 min of post spawning. The best individual treatment produced 64.5% triploid nauplii in cold shock application at a temperature of 10°C for a duration of 8 min. Temperature did not have significant effect (P > 0.05) on triploid rate but duration time had a significant effect (P < 0.05) for individual cold or heat shock. This study demonstrates that because of a wide variety of effective parameters, it is essential to optimize shock conditions for each species strain at each location. © 2016 John Wiley & Sons Ltd.