Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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  • Article
    Validation of New Micro-Spectrophotometric Method To Determine Diamine Oxidase Activity in Serum
    (Springer india, 2024) Sozmen, Eser Yildirim; Sozmen, Alper Baran
    Food allergy and histamine intolerance (HI) are prevalent worldwide. Diamine oxidase (DAO) activity determination in serum is a promising approach for diagnosing HI. Despite the numerous methods that have been published for determining diamine. oxidase activity in serum, most of them have not been validated and have inherent limitations, such as interference and multi-step analysis. Our objective was to perform the analytical validation of a novel micro-spectrophotometric method for the determination of DAO activity in a routine clinical laboratory setting. In this study we determined DAO activity in serum using a 4-aminoantypirine (4-AAP) and 2,4,6 tribromo-3-hidroxybenzoic acid (TBHBA) combination as chromogen for the first time. Validation steps included determination of accuracy (recovery and linearity) and precision (within-run and between-run repeatability) following the Clinical and Laboratory Standards Institute protocols and the other guidelines. Reagent stability assessment at both 4 degrees C temperature and frozen (- 20 degrees C) after 4 h, 24 h, 72 h and a week storage were conducted. The optimum test conditions were determined as pH = 6.0, 37 degrees C, 55 mM Putrescine and 60 min. LOD and LOQ levels were found as 10.08 U/L and 30.56 U/L respectively. Coefficients of variation (CVs) for within-run and between-run precisions were 5.2%, 9.4% for the normal level and 2.6%, 2.2% for the high range, respectively. This reliable method for quantifying DAO activity in serum provides an optimal approach, offering rapid and straightforward performance in laboratories for clinical use.
  • Article
    Citation - WoS: 2
    Citation - Scopus: 2
    Function Generation With Planar Four-Bar Mechanisms as a Mixed Problem of Correlation of Crank Angles and Dead Center Design
    (Springer india, 2024) Kadak, Tarik; Kiper, Gokhan
    Function generation synthesis of mechanisms can be considered as the design of correlation of crank angles and dead dead-center position design. These two problems have been clearly defined and solved separately. But some problems may require both correlation of crank angles and dead dead-center design at different configurations. Such problems are called mixed function generation problems. In this paper, an overview of these mixed function generation problems for the planar four-bar mechanism are given and the problems are solved analytically or semi-analytically. Except three of them, all presented mixed function generation problem formulations are novel. The solutions of all problems including three positions for the four-bar mechanism and the solution of a problem including four positions for a four-bar mechanism are addressed. All problems are first reduced to a univariate equation and a fast solution is found. Thus, link lengths can be found quickly by changing the problem definition problems, and several design iterations can be performed in a short time. Numerical solutions of all problems have been demonstrated using Excel.