Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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Access Right: Open AccessAuthor: search.filters.author.Karakus, Ceyda Oksel

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  • Article
    Citation - Scopus: 1
    Investigating Early-Stage Mineralization Behavior and Bioactivity of Acid-Free Bioactive Glass 45s5 With Enhanced Dissolution Kinetics
    (Springer, 2025) Tuncer, Melisa; Yucesoy, Deniz T.; Karakus, Ceyda Oksel
    Nanostructured bioactive glass (BG) was synthesized through an acid-free sol-gel route (bioglass-AF) and the conventional acid-catalyst sol-gel process (bioglass-AC). The aim here is to eliminate the risk of residual acidic components in the BG while enhancing its functionality through nano-scale propduction. Scanning electron microscopy revealed the presence of highly porous structures and dense agglomerates composed of particles with a mean diameter of 45 nm in both samples. Bioglass-AC and bioglass-AF had specific surface areas of 1.48 m(2)/g and 2.73 m(2)/g, respectively, with an average pore size of similar to 5 nm. Faster mineralization kinetics were evident in bioglass-AF, compared to bioglass-AC, in Hepes-buffered salt solution. Following 14 days of immersion in artificial saliva, bioglass-AC and bioglass-AF lost 16% and 20% of their initial weight, respectively, confirming their bioactivity. None of the synthesized BGs stimulated cell growth up to 24 h but longer exposure to moderate concentrations (1.25 and 2.5 mg/mL) of bioglass-AF significantly enhanced cell viability, reaching 170% at 48 h. Overall, the comparative in vitro investigations proved that nano-structured 45S5 bioglass powders with improved mineralization and dissolution kinetics can be produced with an acid-free route, eliminating the risk of residual acidic components in the final product.
  • Article
    Proliferative Effects and Cellular Uptake of Ceramic Nanoparticles in Cancer and Normal Cells
    (Univ Chemistry & Technology, Prague, 2024) Cesmeli, Selin; Tomak, Aysel; Winkler, David A.; Karakus, Ceyda Oksel
    The high biocompatibility, wear resistance, and high surface area-to-volume ratios of calcium phosphate (CaP) nanoparticles make them materials of great interest for a very broad range of medical applications, such as dentistry, drug delivery, biomedical imaging, gene transfection and silencing, biomedical imaging, immunisation, and bone substitution. While their use as an enamel remineralisation agent, a bone substitution material, an implant coating, and drug/gene delivery agents is widely approved by the regulating bodies, insufficient attention has been paid to the interactions of CaP-based nanoparticles with cells and organs once in the bloodstream and distributed through the body. Here, three different CaP-based nanoparticles (CP: calcium phosphate, TCP: tricalcium phosphate, and HAp: hydroxyapatite) were examined for the proliferative effects, oxidative damage potential, and cellular uptake in the human embryonic kidney (HEK293) and pancreatic cancer (Panc-1) cell lines. The physicochemical properties of the nanoparticles were characterised by Teller analysis, and X-ray diffraction spectroscopy. Maximum proliferative effects were generated by 400 mu g center dot ml-1 TCP (220 %) in HEK293 cells. Interestingly, although CP nanoparticles had the highest reactive oxygen species formation capacity in the HEK293 cells, they exhibited the lowest proliferative effects and a relatively low internalisation rate, suggesting a minimal correlation between the cellular uptake level and oxidative potential.