Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Liposomal Encapsulation of a Synthetic Bromophenol for Antitumor Efficacy and Apoptotic Activity in Cancer Cells(Springer, 2026) Oztanrikulu, Bercem Dilan; Ozdemir, Ekrem; Avci, Bahri; Goksu, Suleyman; Bayrakceken, Handan Uguz; Askin, HakanA novel synthetic bromophenol (BP), inspired by marine-derived natural bromophenols, was evaluated for its antitumor activity and for the enhancement of its in vitro performance through liposomal encapsulation (LipoBP). Etoposide was used as a reference in characterization, release, and loading studies. PEGylated liposomes were employed to improve BP's solubility, bioavailability, and therapeutic potential. The cytotoxicity, apoptosis, and gene expression effects of free BP and LipoBP were assessed in A549 (lung) and MCF-7 (breast) cancer cell lines. WST-8 assays showed that encapsulation significantly increased BP's cytotoxic activity, particularly in A549 cells, while flow cytometry and Annexin V-FITC/PI analyses indicated more pronounced apoptotic induction by LipoBP compared with free BP. qRT-PCR analyses revealed upregulation of proapoptotic genes (BAX, CASP6, CASP3 and CASP9) and downregulation of antiapoptotic/survival genes (BCL-XL, IQSEC2) in both cell lines, indicating activation of intrinsic apoptotic pathways. Plain liposomes exhibited minimal cytotoxicity, confirming their biocompatibility. Liposomal bromophenol, which we have introduced to the literature for the first time, is expected to be a promising nanocarrier system that could be effective in cancer treatment by improving the therapeutic index of new drug candidates such as marine bromophenols.Article Developing Gold Nanoparticles Decorated With Carbon-Dots for Multiplexed Cellular Imaging(IOP Publishing Ltd, 2025) Kavuranpala, Tugce; Saydullaeva, Iroda; Ozcelik, SerdarThis study focuses on developing a novel hybrid nanomaterial composed of gold nanoparticle decorated with carbon dots, termed AuNP@C-dot, as a versatile platform for multiplexed imaging. Structural and spectral characterizations confirmed the successful conjugation of C-dots to AuNPs via covalent bonding, as evidenced by FTIR, X-ray photoelectron spectra, HRTEM analyses, and UV-Vis and fluorescence spectroscopies. The fluorescence intensities of C-dots are doubled through the conjugation to the AuNPs. The conjugation of fluorescent C-dots to plasmon-resonant AuNPs enables simultaneous multicellular imaging by taking advantage of the fluorescent signaling of C-dots and the scattering signaling of AuNPs. In vitro studies using human lung cell lines (A549 and BEAS-2B) confirmed the multiplexed imaging and revealed efficient cellular uptake and subcellular localization of AuNP@C-dots, including nuclear translocation, which is critical for radiotherapy and photodynamic therapy. Cell viability assessments utilizing a colorimetric assay for measuring cell metabolic activity and a colony formation assay demonstrated good biocompatibility of AuNP@C-dots at relevant concentrations. It can be envisioned that the AuNP@C-dot hybrid system may improve the detection and monitoring of cell health and disease due to its dual-modal imaging capability. Furthermore, they could be used for supervising controlled release of therapeutic agents, tailored for enhanced treatment efficacy. This study demonstrates the potential of C-dot-conjugated AuNPs as a multifunctional tool with inherent control mechanisms for the next-generation cellular analysis, drug administration, and diagnostic strategies.