Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 2Citation - Scopus: 2Effect of Mirna Administration on Non-Small Cell Lung Cancer Cells Studied by Cellular Viability Assay and Atr-Ftir Spectroscopy Combined With Multivariate Data-Analysis(Elsevier, 2025) Dagdeviren, Melih; Guler, Gunnur; Guler, Egemen Erdem; Un, Cemal; Karabay-Yavasoglu, Nefise UlkuMicroRNAs (miRNAs), small non-coding RNAs, play a significant role in the regulation of gene expression by various mechanisms. Some miRNAs such as hsa-miR-145 (mir145), hsa-let-7a-1 (let7), hsa-miR-155 (mir155), and hsa-miR-29b (mir29b) are expressed at low levels in cancers and associated with proliferation, metastasis, invasion and apoptosis. In the current study, we aimed to investigate the effect of selected synthetic miRNAs and their combinations on the non-small cell lung cancer (NSCLC) cells (A549) by following the cell viability profile and alterations in the cellular biomolecules with biophysical features. After administration of commercial miRNAs and their various combinations to A549 cell line, each group was analyzed with cell viability assay and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with unsupervised multivariate analysis. Bioinformatics analysis was also performed to detect and to classify the target human genes obtained from the mirDB database. According to the cell viability results, the "mir29b + let7" combination and "mir155" significantly decreased the cancer cell viability whereas the "mir145 + mir29b" and "mir155 + mir145" combinations dramatically increased the cancer cell viability when compared to the control cells. The FTIR data revealed that administration of the "mir155", "mir29b + let7 + mir155", and "mir29b + let7" combinations caused a decrease in the contents of proteins, lipids and nucleic acids in A549 cells. This study suggests that those miRNA combinations might be potential targets for vaccines or miRNA-based therapies that can restore the miRNA activity and thus should be further evaluated to combat lung cancer with miRNA technology.Article Citation - WoS: 4Citation - Scopus: 3An Investigation of Rna Methylations With Biophysical Approaches in a Cervical Cancer Cell Model(Mdpi, 2024) Saglam, Buket; Akkus, Onur; Akcaoz-Alasar, Azime; Ceylan, Cagatay; Guler, Gunnur; Akgul, BunyaminRNA methylation adds a second layer of genetic information that dictates the post-transcriptional fate of RNAs. Although various methods exist that enable the analysis of RNA methylation in a site-specific or transcriptome-wide manner, whether biophysical approaches can be employed to such analyses is unexplored. In this study, Fourier-transform infrared (FT-IR) and circular dichroism (CD) spectroscopy are employed to examine the methylation status of both synthetic and cellular RNAs. The results show that FT-IR spectroscopy is perfectly capable of quantitatively distinguishing synthetic m(6)A-methylated RNAs from un-methylated ones. Subsequently, FT-IR spectroscopy is successfully employed to assess the changes in the extent of total RNA methylation upon the knockdown of the m(6)A writer, METTL3, in HeLa cells. In addition, the same approach is shown to accurately detect reduction in total RNA methylation upon the treatment of HeLa cells with tumor necrosis factor alpha (TNF-alpha). It is also demonstrated that m(1)A and m(6)A methylation induce quite a distinct secondary structure on RNAs, as evident from CD spectra. These results strongly suggest that both FT-IR and CD spectroscopy methods can be exploited to uncover biophysical properties impinged on RNAs by methyl moieties, providing a fast, convenient and cheap alternative to the existing methods.Book Part Citation - WoS: 5Citation - Scopus: 4Surface Characterization Techniques(Wiley-v C H Verlag Gmbh, 2017) Erdogan, Gokhan; Guler, Gunnur; Kilic, Tugba; Kilic, Duygu O.; Erdogan, Beyhan; Tosun, Zahide; Karaman, Mustafa