Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Fabrication of Superhydrophilic Teos-Lactic Acid Composite Films and Investigation of Biofouling Behaviour(Yıldız Teknik Üniversitesi, 2022) Ervan, Tuğçe; Küçüker, Mehmet Ali; Cengiz, Uğur; 03.07. Department of Environmental Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyPhytoplankton and diatom microalgae species cause biofouling by adhering to the surfaces, especially in closed cultivation systems such as tubular photobioreactors. This biofilm formation blocks the sunlight; after harvesting, it is necessary to clean the reactor. This cleaning process causes loss not only for time and finance but also in terms of environmental pollution due to using toxic chemicals and excess water usage. This study aimed to investigate the reduction of the microorganism cell adhesion on the hybrid surface. To succeed in this, the composite surface of tetraethoxysilane (TEOS) and lactic acid (LA) was prepared by the sol-gel process. Then the hybrid surfaces were coated on glass slides by the dip coating method. The wettability performance of the TEOS-LA hybrid surface was investigated using contact angle measurement and light transmittance. The wettability result showed that the superhydrophilic surface having 54 mJ/m2 of surface free energy values was obtained. An increase in the lactic acid content of the composite films increased the surface free energy (SFE) values decreasing the water contact angle. A pencil hardness test characterized the mechanical strength of the surfaces, and it was determined that the hardness of the composite films was decreased by increasing the LA content of the composite films. Resultantly, it is found that the TEOS-LA superhydrophilic composite film reduces the adhesion of microalgae.Article Citation - WoS: 8Citation - Scopus: 9Development of Plant-Based Biopolymer Coatings for 3d Cell Culture: Boron-Silica Quince Seed Mucilage Nanocomposites(Royal Society of Chemistry, 2023) Yılmaz, Hilal Deniz; Cengiz, Uğur; Derkuş, Burak; Arslan, Yavuz Emre; 01. Izmir Institute of TechnologySpheroid formation with spontaneous aggregation has captured interest in most cell culture studies due to its easy set-up and more reliable results. However, the economic and technical costs of the advanced systems and commercial ultra-low adhesive platforms have pushed researchers into pursuing alternatives. Nowadays, polymeric coatings, including poly-hydroxyethyl methacrylate and agar/agarose, are the commonly used polymers for non-adhesive plate fabrication, yet the costs and working solvent or heat-dependent preparation procedures maintain the need for the development of novel biomaterials. Here, we propose a greener and more economical approach for producing non-adherent surfaces and spheroid formation. For this, a plant waste-based biopolymer from quince fruit (Cydonia oblonga Miller, from Rosaceae family) seeds and boron-silica precursors were introduced. The unique water-holding capacity of quince seed mucilage (Q) was enriched with silanol and borate groups to form bioactive and hydrophilic nanocomposite overlays for spheroid studies. Moreover, 3D gel plates from the nanocomposite material were fabricated and tested in vitro as a proof-of-concept. The surface properties of coatings and the biochemical and mechanical properties of the nanocomposite materials were evaluated in-depth with techniques, and extra hydrophilic coatings were obtained. Three different cell lines were cultured on these nanocomposite surfaces, and spheroid formation with increased cellular viability was recorded on day 3 with a >200 & mu;m spheroid size. Overall, Q-based nanocomposites are believed to be a fantastic alternative for non-adherent surface fabrication due to their low-cost, easy operation, and intrinsic hydration layer forming capacity with biocompatible nature in vitro.