Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

Browse

Filters

2024 - 20266

Settings

Author: search.filters.author.Gulec, SukruStart date: 2024

Search Results

Now showing 1 - 6 of 6
  • Article
    FTIR Spectroscopy Coupled With Chemometrics for Evaluating Functional Food Efficacy in an in Vitro Model of Iron Deficiency Anemia
    (Elsevier Science Ltd, 2026) Dalyan, Eda; Cavdaroglu, Cagri; Ozen, Banu; Gulec, Sukru
    Vibrational spectroscopy offers a rapid, cost-effective approach for studying biological systems. This study employs Fourier Transform Infrared (FTIR) spectroscopy, combined with Soft Independent Modeling of Class Analogy (SIMCA), to evaluate treatment outcomes for iron deficiency anemia (IDA). The model was built using spectra from healthy and anemic cells, then validated with cells treated with commonly used iron supplements. In calibration, 9 of 10 control and all IDA samples were correctly classified; 14 of 15 validation samples were identified as healthy. The model was applied to cells treated with protein-iron complexes. All samples treated with a 60:1 protein-iron ratio matched the healthy group, while 3 of 4 treated with a 10:1 ratio matched the IDA group. These results were further supported by iron-regulated gene expression of transferrin receptor (TFR) and (Ankyrin Repeat Domain 37) ANKRD37. FTIR coupled with chemometrics enables rapid assessment of functional effects and shows potential for screening functional ingredients in anemia-targeted food products.
  • Article
    Chain-Length Dependent and Synergistic Prebiotic Effects of Xylooligosaccharides and Xylan on the Fecal Microbiota of Mice in Vitro
    (Elsevier, 2025) Sabanci, Kevser; Gulec, Sukru; Buyukkileci, Ali Oguz
    Oligomeric and polymeric prebiotics differ in their structural complexity, which influences microbial accessibility and fermentation kinetics. This study investigated the microbial responses to xylooligosaccharides (XOS), xylan (XY), and their combinations in comparison with inulin (INU) using an in vitro model inoculated with BALB/c mice fecal microbiota. Temporal analyses over 48 h included substrate consumption, acid production, and changes in microbial diversity. XOS was rapidly fermented, yielding high acetate and lactate levels, whereas XY was utilized more slowly due to its polymeric structure. During XY fermentation, xylobiose (X2) and xylotriose (X3) accumulated transiently, suggesting a stepwise depolymerization and utilization mechanism. The XOS + XY mix showed enhanced prebiotic effect, producing the highest amount of acid (151.8 mmol/L) and notably promoted the simultaneous enrichment of Bifidobacterium (12.5-fold), Bacteroides (8.85-fold), and Lactobacillus (14.9-fold) species compared to individual treatments These findings demonstrate that coadministered XOS and XY highlights the potential for designing tailored prebiotic formulations to optimize microbiota modulation, with potential relevance for human health.
  • Article
    Sainfoin (Onobrychis Viciifolia L.) Protein Isolate as a New Source of Alternative Plant-Based Protein: Cytotoxicity, Immunoreactivity, Nutritional and Functional Properties
    (Springer, 2025) Korkmaz, Fatma; Gungor, Sevde Nur; Gulec, Sukru; Sakarya, Fatma Betul; Andac, Ali Emre; Yilmaz Tuncel, Nese; Tuncel, Necati Baris
    The objective of this research was to develop an alternative plant-based protein isolate using sainfoin (Onobrychis viciifolia L.) seeds. The extraction process was optimized using response surface methodology (RSM) based on the Box-Behnken Design, which examined the effects of key parameters: solvent/solid ratio (10-50 mL/g), pH (8-11), temperature (20-50 degrees C), and extraction time (30-120 min), aiming to maximize protein yield. The optimal extraction conditions identified were a solvent/solid ratio of 49.96 mL/g, pH of 10.99, temperature of 20 degrees C, and a duration of 38.55 min, achieving a protein yield of 56.36%. Additionally, the amino acid composition, cytotoxicity, immunoreactivity, and functional properties of the sainfoin seed protein isolate (SPI) were evaluated. SPI exhibited a high crude protein content of 91.44%, with arginine being the most abundant amino acid at 158.20 mg/g. The protein isolate comprised a remarkable value of 50.26% essential amino acids. Additionally, SPI demonstrated desirable functional properties, including solubility of 53.95% at neutral pH, water holding capacity of 2.36 g/g, and oil binding capacity of 4.68 g/g. Its emulsifying performance was notable, with emulsion activity and stability values of 66.67% and 77.50%, respectively. Moreover, in vitro cell culture studies demonstrated that sainfoin seed protein exhibited no adverse effects on cellular toxicity or immunoreactivity. This study highlights the potential of SPI as a novel, high-quality plant protein source with promising nutritional and functional properties and demonstrates its potential as a functional ingredient in the formulation of plant-based foods, meat analogs, and dietary supplements.
  • Article
    Antidiabetic and Anticancer Properties of Sun-Dried Fig (Ficus Carica) Stalk Pectin: Effects on Intestinal Glucose Absorption and Colon Cancer Cell Growth
    (Elsevier, 2025) Baser, Filiz; Cavdaroglu, Elif; Yemenicioglu, Ahmet; Gulec, Sukru
    This study aims to characterize the physiological activity of fig stalk pectin (FSP) in terms of antidiabetic and anticancer activities. Also, the potency of FSP has been interpreted as a functional food ingredient in yogurt. The galacturonic acid content (65 %), degree of esterification (63 %), and enzymatic sugar analysis showed that FSP is a high methoxyl pectin rich in RG-I content (similar to 22 %). Anti-diabetic characteristics of FSP demonstrated that FSP inhibited 2-deoxyglucose uptake into CaCo-2 cells and reduced glucose absorption in the intestinal transport system after being added as an ingredient in yogurt at the concentration of 2 % (w/w). The antidiabetic activity of FSP was attributed to its capacity to modify the rheological properties of yogurt with a high-water binding capacity (10 g/g), and it increased the viscosity of digested yogurt samples considerably (from 89 to 110 Cp). Moreover, the characterization of anticancer properties showed that FSP inhibited the proliferation of colon cancer CaCo-2 cells by disturbing cell cycle progression, leading to S phase arrest, and showing apoptosis-inducing ability. Further research, including in vivo and clinical trials, is necessary to validate the observed health benefits of FSP.
  • Article
    Citation - WoS: 3
    Citation - Scopus: 3
    An Ex Vivo Model for Evaluation of Prebiotic Activity of Xylan and Xylooligosaccharides
    (Elsevier, 2025) Sabanci, Kevser; Gulec, Sukru; Buyukkileci, Ali Oguz
    Ex vivo techniques can provide more physiologically significant insights into prebiotic activity and overcome some limitations of in vitro tests. In this study, an ex vivo model, formed of a large intestine of mice, was tested to assess the effects of the hydrocolloidal natural polymer, xylan (XY), and its hydrolysis product, xylooligosaccharides (XOS). XY and XOS were loaded separately into the cecum, proximal colon, and distal colon. Their utilization and short-chain fatty acid (SCFA) formation by the colonized microflora and levels of dominant phyla and key genera such as Bifidobacterium, Bacteroides, and Lactobacillus were followed. XY and XOS were metabolized in all sections, and SCFAs were released. The results suggest that the slower utilization of XY compared to XOS in the cecum can enable this polysaccharide to move towards distal parts of the large intestine and extend the sites of prebiotic activity. Unlike widely used in vitro models, the ex vivo model allowed testing the utilization pattern and effects of the prebiotics in the natural environment of the microflora and examining the intestinal sections separately.
  • Article
    Citation - WoS: 3
    Citation - Scopus: 3
    Development of Xylan-Coated Acid-Resistant Micellar Drug Carriers for Colon-Targeted Oral Delivery
    (Taylor & Francis As, 2024) Zeybek, Nuket; Polat, Hurriyet; Gulec, Sukru; Buyukkileci, Ali Oguz
    Oral delivery of hydrophobic drugs from the stomach through the colon has some requirements: (1) an acid-resistant carrier (2) a colon-specific drug release mechanism; and (3) an enhanced bioavailability. In this study, curcumin-loaded polymeric micelles with a xylan-based composite coating were designed and developed. For this purpose, a new synthesis method was used to precipitate xylan by concurrent chitosan polymerization at different xylan/chitosan ratios using a negatively charged crosslinking agent, TPP. The study was to provide the stability of the coated micellar structures in the stomach (low pH conditions) and their degradation in the colon (a natural environment of bacteria) to release the drug. It was observed that the coating successfully prevented early drug release up to 85%, depending on the fraction of xylan in the coating. The nanocarriers that first passed through the stomach conditions were incubated with a xylanolytic colonic bacterium (Bacteroides ovatus) to determine the bacterium-related release mechanism, which was around 27%. This shows the colon-specific release expectation of coated nanocarriers in the colon environment, with an additional benefit due to the degradation of xylan and an improvement in the colon environment by prebiotic activity.