Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
Browse
3 results
Search Results
Article FTIR Spectroscopy Coupled With Chemometrics for Evaluating Functional Food Efficacy in an in Vitro Model of Iron Deficiency Anemia(Elsevier Science Ltd, 2026) Dalyan, Eda; Cavdaroglu, Cagri; Ozen, Banu; Gulec, SukruVibrational spectroscopy offers a rapid, cost-effective approach for studying biological systems. This study employs Fourier Transform Infrared (FTIR) spectroscopy, combined with Soft Independent Modeling of Class Analogy (SIMCA), to evaluate treatment outcomes for iron deficiency anemia (IDA). The model was built using spectra from healthy and anemic cells, then validated with cells treated with commonly used iron supplements. In calibration, 9 of 10 control and all IDA samples were correctly classified; 14 of 15 validation samples were identified as healthy. The model was applied to cells treated with protein-iron complexes. All samples treated with a 60:1 protein-iron ratio matched the healthy group, while 3 of 4 treated with a 10:1 ratio matched the IDA group. These results were further supported by iron-regulated gene expression of transferrin receptor (TFR) and (Ankyrin Repeat Domain 37) ANKRD37. FTIR coupled with chemometrics enables rapid assessment of functional effects and shows potential for screening functional ingredients in anemia-targeted food products.Article Chain-Length Dependent and Synergistic Prebiotic Effects of Xylooligosaccharides and Xylan on the Fecal Microbiota of Mice in Vitro(Elsevier, 2025) Sabanci, Kevser; Gulec, Sukru; Buyukkileci, Ali OguzOligomeric and polymeric prebiotics differ in their structural complexity, which influences microbial accessibility and fermentation kinetics. This study investigated the microbial responses to xylooligosaccharides (XOS), xylan (XY), and their combinations in comparison with inulin (INU) using an in vitro model inoculated with BALB/c mice fecal microbiota. Temporal analyses over 48 h included substrate consumption, acid production, and changes in microbial diversity. XOS was rapidly fermented, yielding high acetate and lactate levels, whereas XY was utilized more slowly due to its polymeric structure. During XY fermentation, xylobiose (X2) and xylotriose (X3) accumulated transiently, suggesting a stepwise depolymerization and utilization mechanism. The XOS + XY mix showed enhanced prebiotic effect, producing the highest amount of acid (151.8 mmol/L) and notably promoted the simultaneous enrichment of Bifidobacterium (12.5-fold), Bacteroides (8.85-fold), and Lactobacillus (14.9-fold) species compared to individual treatments These findings demonstrate that coadministered XOS and XY highlights the potential for designing tailored prebiotic formulations to optimize microbiota modulation, with potential relevance for human health.Article Citation - WoS: 3Citation - Scopus: 3An Ex Vivo Model for Evaluation of Prebiotic Activity of Xylan and Xylooligosaccharides(Elsevier, 2025) Sabanci, Kevser; Gulec, Sukru; Buyukkileci, Ali OguzEx vivo techniques can provide more physiologically significant insights into prebiotic activity and overcome some limitations of in vitro tests. In this study, an ex vivo model, formed of a large intestine of mice, was tested to assess the effects of the hydrocolloidal natural polymer, xylan (XY), and its hydrolysis product, xylooligosaccharides (XOS). XY and XOS were loaded separately into the cecum, proximal colon, and distal colon. Their utilization and short-chain fatty acid (SCFA) formation by the colonized microflora and levels of dominant phyla and key genera such as Bifidobacterium, Bacteroides, and Lactobacillus were followed. XY and XOS were metabolized in all sections, and SCFAs were released. The results suggest that the slower utilization of XY compared to XOS in the cecum can enable this polysaccharide to move towards distal parts of the large intestine and extend the sites of prebiotic activity. Unlike widely used in vitro models, the ex vivo model allowed testing the utilization pattern and effects of the prebiotics in the natural environment of the microflora and examining the intestinal sections separately.