Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 1The Impact of Oxygen and Antimicrobial Tea Tree Oil Carrying Biomaterial on Cell Viability Under Hypoxic Conditions(Wiley, 2025) Demir, Yagmur Damla; Tepeli, Dilek; Guvensen, Mahmut Deniz; Soyer, Ferda; Akin, Ozlem; Kehr, Nermin SedaTraditional wound treatment involves protecting the wound with dressing and administering antibiotics to prevent tissue infection due to bacteria. However, these methods are inadequate due to the side effects of antibiotics on healthy cells and microbial resistance to antibiotics. Therefore, new strategies involving the application of natural resources such as essential oils as antimicrobial agents in combination with biomaterials as wound dressings have been tested in the treatment of wounds. Furthermore, oxygen (O2)-releasing biomaterials have attracted great interest due to the important role of O2 in wound healing processes. However, the co-application of O2 and essential oil as antimicrobial and cell-promoting agents has not been studied. In this context, we report a novel biomaterial capable of co-delivering O2 and natural antimicrobial tea tree oil (TTO) for 15 and 5 days, respectively. The biomaterial consists of an alginate scaffold (Alg-PMOF-O) containing O2-carrying nanomaterial, laponite and TTO. In vitro bacterial experiments have shown that O2 release from Alg-PMOF-O is an additional parameter acting as an antibacterial agent to inhibit bacterial growth but is not sufficient alone to inhibit bacteria. 5 mu L of TTO in Alg-PMOF-O is necessary to suppress both E. coli and S. aureus over a 1-day incubation period. The effect of TTO and O2 alone or in combination on cell viability is examined using WST-1 and PrestoBlue assays. According to the WST-1 and PrestoBlue tests, the combined application of TTO and O2 does not show any toxic effect on fibroblast cells under normoxic conditions during the 5-day incubation period. Under hypoxic conditions, the WST-1 test shows no toxic effect after only 1 day of incubation, while the PrestoBlue test shows no toxicity under hypoxia during both 1 and 5 days of incubation. On the other hand, the combined application of TTO and O2 indicates toxic effects on cancer Malme-3M cells during both normoxic and hypoxic conditions over 1 and 5 days of incubation. This effect is confirmed by both the WST-1 and PrestoBlue tests. The overall results demonstrate that Alg-PMOF-O exhibits antibacterial activity while having a lower toxic effect on fibroblasts under hypoxic conditions, and therefore has potential for use as wound dressing.Article Citation - WoS: 7Citation - Scopus: 6Polymeric Biomaterials for Periodontal Tissue Engineering and Periodontitis(Royal Soc Chemistry, 2024) Yuruk, Gizem; Demir, Yagmur Damla; Vural, Sevra; Kehr, Nermin SedaThe periodontium is one of the most complex tissues in the body because its structure is formed by a hierarchical combination of soft and hard tissues. Due to its complex architecture, the treatment and regeneration of damaged periodontal tissue caused by diseases is still a challenge in biomedicine. The most common disease of the periodontium is periodontitis, which occurs when the periodontium becomes infected and inflamed as a bacterial biofilm forms in the mouth. Recently, various biocompatible biomaterials made of natural and synthetic polymers have been developed for periodontal tissue regeneration or treatment due to their superior properties such as controlled drug and bioactive molecule delivery, mimicking the 3D network of tissue, biocompatibility, antibacterial and mechanical properties. In particular, biomaterials designed for drug delivery, such as hydrogels, scaffolds, films, membranes, micro/nanoparticles and fibers, and additively manufactured biomaterials have undergone in vitro and in vivo testing to confirm their potential clinical utility in periodontal regeneration and periodontitis treatment. This review explores recent advances in the use of biomaterials for the prevention and/or treatment of periodontal regeneration and periodontitis. Specifically, it emphasizes advancements in drug/biomolecule delivery and the use of additively manufactured biomaterials for addressing periodontal issues.Article Citation - WoS: 2Citation - Scopus: 4Injectable Nanocomposite Hydrogels With Co-Delivery of Oxygen and Anticancer Drugs for Higher Cell Viability of Healthy Cells Than Cancer Cells Under Normoxic and Hypoxic Conditions(Iop Publishing Ltd, 2025) Kehr, Nermin SedaInjectable nanocomposite hydrogels (NC hydrogels) have the potential to be used for minimally invasive local drug delivery. In particular, pH-sensitive injectable NC hydrogels can be used in cancer treatment to deliver high doses of anticancer drugs to the target site in cancer tissue without damaging healthy tissue. Recent studies have shown that in addition to stimuli-responsive delivery of anticancer drugs to cancer cells, oxygen delivery to the hypoxic environment of cancer tissue can lead to advanced effects, as hypoxia and an acidic pH are common characteristics of cancer tissue. However, few studies have investigated the effects of simultaneous administration of oxygen (O2) and pH-dependent anticancer drugs via injectable NC hydrogels on the viability of healthy and cancer cells under normoxic and hypoxic conditions. In this context, we describe the synthesis of injectable NC hydrogels composed of pH-responsive nanomaterials carrying oxygen and anticancer drugs. Our system provides sustained O2 release and pH-responsive sustained release of anticancer drugs for 15 and 30 d, respectively. Moreover, O2 delivery and/or simultaneous delivery of O2 and anticancer drug resulted in higher cell survival of healthy fibroblast cells than malignant Colo-818 cells under hypoxic conditions (1% O2) after 7 d of incubation.Article Citation - WoS: 3Citation - Scopus: 3The Effect of Co-Delivery of Oxygen and Anticancer Drugs on the Viability of Healthy and Cancer Cells Under Normoxic and Hypoxic Conditions(Wiley-v C H verlag Gmbh, 2024) Kehr, Nermin SedaHypoxia, cancer, tissue damage, and acidic pH conditions are interrelated, as chronic hypoxic conditions enhance the malignant phenotype of cancer cells, causing more aggressive tissue destruction, and hypoxic cells rely on anaerobic glycolysis, leading to the accumulation of lactic acid. Therefore, the administration of oxygen is necessary to support the functions of healthy cells until the formation of new blood vessels and to increase the oxygen supply to cancerous tissues to improve the efficacy of antitumor drugs on tumor cells. In addition to O2 supply, pH-dependent delivery of anticancer drugs is desired to target cancer cells and reduce drug side effects on healthy cells. However, the simultaneous delivery of O2 and pH-dependent anticancer drugs via nanomaterials and their effects on the viability of normal and cancer cells under hypoxic conditions have not been studied in sufficient numbers. This study describes the synthesis of a pH-responsive nanomaterial containing oxygen and anticancer drugs that exhibits sustained O2 release over a 14 d period under hypoxic conditions and pH-dependent sustained release of anticancer drugs over 30 d. The simultaneous administration of O2 and anticancer drugs results in higher cell survival of normal cells than that of cancer cells under hypoxic and normoxic conditions. The synthesis of pH-sensitive perfluorocarbon-based nanomaterials containing oxygen and anticancer drugs is reported. These nanomaterials provide oxygen release within 14 d and pH-dependent sustained release of anticancer drugs over 30 d. Co-delivery of oxygen and anticancer drugs by pH-sensitive nanomaterials promotes the viability of healthy cells compared to malignant cells under hypoxic conditions. image