Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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Author: search.filters.author.Okkali, Gaye SumerPublication Index: search.filters.publicationIndex.Scopus

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  • Article
    Enhancement of Corchorus Olitorius L. on Osteogenic Differentiation of MC3T3-E1 Pre-Osteoblast Cells by Increasing Alkaline Phosphatase and Hydroxyproline
    (Taylor & Francis Ltd, 2025) Ertugruloglu, Pinar; Baris, Elif; Okkali, Gaye Sumer; Boke Sarikahya, Nazli
    Corchorus olitorius L. (jute mallow or molehiya) belongs to the Malvaceae family valued for its nutritional and medicinal properties. In this study, the potential to enhance osteogenesis in MC3T3-E1(Murine Calvaria-derived 3T3 Subclone E1) pre-osteoblastic cells was investigated to support bone formation and mineralisation. Leaf ethanolic extract was prepared and applied to MC3T3-E1 cells. Osteogenic effects were evaluated through three methods: MTT assays for cell viability, Alizarin Red S staining for calcium deposition, enzymatic analyses for alkaline phosphatase (ALP) and hydroxyproline (HYP). A non-cytotoxic concentration of C. olitorius extract (0.5 mg/mL) significantly increased ALP and HYP levels, promoting osteogenic differentiation in both undifferentiated and differentiated cells. HYP levels were notably elevated in differentiated cells. The findings suggested that C. olitorius extract may be a promising natural agent for enhancing bone health, warranting further in vivo and clinical studies to confirm its therapeutic potential.
  • Article
    Citation - WoS: 2
    Citation - Scopus: 1
    Isolation, Purification, Structural Characterization, and Encapsulation of Sulforaphane From Cabbage Seeds Using the Ionic Gelation Method
    (Springer, 2025) Oner, Beste; Aydin, Ozgun Koprualan; Okkali, Gaye Sumer; Sarikahya, Nazli Boke; Nadeem, Hilal Sahin; KaymakErtekin, Figen
    Sulforaphane (SFN), a phytochemical with anticancer and antibacterial properties, is a secondary metabolite found in cabbage (both in the plants and seeds), a member of the Brassica (crusifer) family. However, pure sulforaphane is volatile, sensitive to pH, temperature, light, and oxygen, and is also only oil-soluble, limiting its stability and bioaccessibility. Therefore, encapsulation is required to enhance stability and control its release. In this study, sulforaphane-rich extracts were first obtained from cabbage seeds using a hybrid ultrasonic-microwave extraction method yielding 24.35%, with a sulforaphane content of 8202.68 mu g/g DM, followed by purification of sulforaphane through chromatography. Structural elucidation was performed using H-1-NMR, C-13-NMR, and QTOF LC/MS. The pure SFN (> 90% purity) was encapsulated using the ionic gelation method to improve its bioaccessibility and stability. Various coating material combinations, sodium alginate/chitosan and sodium alginate/pectin were evaluated for the encapsulation process. Fourier-transform infrared spectroscopy (FTIR) was used to investigate the relationship between the coating materials, and the most efficient combination was selected. Optimization of process variables using central composite rotatable design (CCRD) identified the optimal conditions: 1.41% sulforaphane-oil mixture, 0.18% chitosan, and 6.88 min waiting time. Under these conditions, microcapsules were produced with high encapsulation efficiency (76.02%) and low solubility (4.78%). & Idot;n vitro bioaccessibility studies further confirmed minimal sulforaphane release during the oral and gastric phases, with a low release in the intestinal phase. These results demonstrate the effectiveness of the encapsulation system in protecting sulforaphane under harsh gastrointestinal conditions.