Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 2Citation - Scopus: 3Dynamic Fluidic Manipulation in Microfluidic Chips With Dead-End Channels Through Spinning: the Spinochip Technology for Hematocrit Measurement, White Blood Cell Counting and Plasma Separation(Royal Soc Chemistry, 2025) Oksuz, Cemre; Tekin, Hüseyin Cumhur; Bicmen, Can; Tekin, H. CumhurCentrifugation is crucial for size and density-based sample separation, but low-volume or delicate samples suffer from loss and impurity issues during repeated spins. We introduce the "Spinochip", a novel microfluidic system utilizing centrifugal forces for efficient filling of dead-end microfluidic channels. The Spinochip enables versatile fluid manipulation with a single reservoir for both inlet and outlet functions. It expels compressed air, facilitating fluid flow, and offers programmable filling mechanisms based on the hydraulic resistance of microfluidic channels. Compatible with a basic centrifuge, it allows sequential filling, internal mixing, and collection in straight microfluidic channels by simply adjusting the spinning speed, eliminating the need for complex valving. We demonstrated the Spinochip's efficacy in blood testing, where it successfully separated blood components, such as plasma, buffy coat, and red blood cells, from a single drop using centrifugation alone. This system enabled simultaneous hematocrit (R2 >0.99) and total white blood cell (R2 >0.93) quantification within a single microfluidic channel without the need for staining or special reagents. Remarkably, the Spinochip can perform hematocrit measurements on as little as 100 nL of blood, potentially paving the way for less invasive blood analysis. This innovative approach unlocks new possibilities in microfluidics, providing precise fluidic control and centrifugation for sample volumes as small as a few nanoliters.Conference Object Citation - Scopus: 1Colorimetric Detection of Creatinine on an Electromechanical Mixing Platform(IEEE, 2021) Tarim, E. Alperay; Oksuz, Cemre; Karakuzu, Betul; Tekin, H. CumhurWe present an electromechanical mixing platform integrated with a smartphone for colorimetric detection of creatinine using an enzymatic reaction between horseradish peroxidase (HRP) and 3,3',5,5'-Tetramethylbenzidine (TMB). On the developed platform, the color resulting from the reaction between HRP and TMB in a reservoir of a poly(methyl methacrylate) (PMMA) chip is measured using a smartphone camera. With mixing, diluted creatine solutions can be detected in 1 min that can reduce long waiting times of creatinine detection due to enzymatic reactions. Furthermore, smartphone-based colorimetric detection reduces the cost of analysis by eliminating costly equipment for spectrometric measurements without affecting the sensitivity of analysis. We, therefore, think that the presented platform integrated with a smartphone could be used for automatic measurement of creatinine level in the sample by allowing low-cost and rapid analysis, and this would be particularly beneficial for monitoring of chronic kidney disease (CKD) at the point-of-care setting.