Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Book Part Sample Preparation Using Microfluidic Technologies for Non-Invasive Tests(Elsevier, 2025) Oksuz, C.; Tarim, E.A.; Ozcan, H.A.; Koc, S.; Tekin, H.C.The collection of a biological sample and the steps carried out to obtain the target in a sample covers the sample preparation procedures which are one of the important steps for diagnostic tests. Removing interferences in a complex sample, preventing undesirable reactions, separating, purifying, and enriching the sample are among the steps that can be applied to samples for analysis. Non-invasive tests include samples such as urine, saliva, sweat, tear, breath and are preferred because they are simple, painless, cost-effective and cause fewer complications. In traditional methods applied in clinics, most of the steps such as centrifugation, pipetting, staining, and washing are performed manually by a technician. For this reason, tests are costly, require long analysis time, and have a significant risk of contamination and manual errors. Microfluidic technologies allow automating sample collection and preparation steps by integrating many components on a single chip. Thereby, low-volume samples can be processed automatically with high efficiency and purity. In this chapter, the sample preparation methods used in microfluidic devices for non-invasive tests analyzing human samples including sweat, urine, saliva, tears, sexual samples, and other body fluids are reviewed. This information aims to facilitate the development of potential sample preparation methods and applications for non-invasive diagnostic tests. © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.Conference Paper Magnetic Levitation-Based Endothelial Cell Sorting(Institute of Electrical and Electronics Engineers Inc., 2023) Ozcan, H.A.; Kecili, S.; Tekin, H.C.Cell sorting for rare cells is crucial for diagnostic purposes. Circulating Endothelial Cells (CECs) can be used as cardiovascular disease markers. Due to the rareness of the CECs in the blood, an accurate, easy, cost and time-effective sorting method is a need. Magnetic levitation is a promising technique for observing differences in the average height of endothelial and white blood cells which does not require any labeling. This study aims to show that the magnetic levitation principle can be used for sorting endothelial cells from the blood. By using paramagnetic medium concentrations of 10 mM and 50 mM, the average levitation height between HUVECs used as a model endothelial cells and U937 cells used as model of white blood cells was measured as 65 μm and 32 μm, respectively. Since there is a significant difference in levitation height for HUVECs and U937 cells, magnetic levitation technology exhibits promising potential for the precise sorting of endothelial cells. © 2023 IEEE.