Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

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Author: search.filters.author.Ozcelik, SerdarPublication Index: search.filters.publicationIndex.PubMed

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  • Article
    Developing Gold Nanoparticles Decorated With Carbon-Dots for Multiplexed Cellular Imaging
    (IOP Publishing Ltd, 2025) Kavuranpala, Tugce; Saydullaeva, Iroda; Ozcelik, Serdar
    This study focuses on developing a novel hybrid nanomaterial composed of gold nanoparticle decorated with carbon dots, termed AuNP@C-dot, as a versatile platform for multiplexed imaging. Structural and spectral characterizations confirmed the successful conjugation of C-dots to AuNPs via covalent bonding, as evidenced by FTIR, X-ray photoelectron spectra, HRTEM analyses, and UV-Vis and fluorescence spectroscopies. The fluorescence intensities of C-dots are doubled through the conjugation to the AuNPs. The conjugation of fluorescent C-dots to plasmon-resonant AuNPs enables simultaneous multicellular imaging by taking advantage of the fluorescent signaling of C-dots and the scattering signaling of AuNPs. In vitro studies using human lung cell lines (A549 and BEAS-2B) confirmed the multiplexed imaging and revealed efficient cellular uptake and subcellular localization of AuNP@C-dots, including nuclear translocation, which is critical for radiotherapy and photodynamic therapy. Cell viability assessments utilizing a colorimetric assay for measuring cell metabolic activity and a colony formation assay demonstrated good biocompatibility of AuNP@C-dots at relevant concentrations. It can be envisioned that the AuNP@C-dot hybrid system may improve the detection and monitoring of cell health and disease due to its dual-modal imaging capability. Furthermore, they could be used for supervising controlled release of therapeutic agents, tailored for enhanced treatment efficacy. This study demonstrates the potential of C-dot-conjugated AuNPs as a multifunctional tool with inherent control mechanisms for the next-generation cellular analysis, drug administration, and diagnostic strategies.
  • Article
    Citation - WoS: 2
    Citation - Scopus: 2
    Vibrational Spectroscopy Unveils Distinct Cell Cycle Features of Cancer Stem Cells in Melanoma
    (Nature Portfolio, 2025) Uslu, Bensu Ruya; Ozdil, Berrin; Tarhan, Enver; Ozcelik, Serdar; Aktug, Huseyin; Guler, Gunnur
    Cancer stem cells (CSCs) play a central role in melanoma growth, resistance to treatment, and relapse, however, their dynamic regulatory behavior remains poorly understood. Vibrational spectroscopy offers a unique, label-free approach to investigate cellular heterogeneity at the molecular level. Here, we explored the biochemical and regulatory dynamics of CSCs identified by using a time-course design, integrating infrared and Raman spectroscopies with cell cycle analysis and immunocytochemistry targeting the checkpoint proteins p16 and p21. CSCs, non-cancer stem cells (NCSCs), and bulk CHL-1 melanoma cells were monitored at 11, 24, 48, and 72 h. CSCs showed a steady S-phase with an early rise in p16 followed by a subsequent increase in p21 expression, indicating a dynamic state of cell cycle checkpoints. In contrast, NCSCs and CHL-1 cells showed more transient p16/p21 expression and CHL-1 exhibited a marked p16 increase at 24 h. Spectroscopic analysis revealed that CSCs exhibited distinct vibrational profiles, predominantly in the nucleic acid-, protein- and lipid-associated regions. These differences were further supported by principal component and hierarchical clustering analyses, which consistently distinguished CSCs from NCSCs. Our findings underline the potential of vibrational spectroscopy to sensitively detect CSC-specific regulatory patterns and support its use in detecting new therapeutic targets in melanoma.