Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
Browse
2 results
Search Results
Book Part Computational Approaches for Antibody-Based Drug Design(Institute of Physics Publishing, 2024) Gunes, S.; Uyar, Arzu; Arkca, I.; Güneş, Seda; Atik, S.B.; Ulucan-Karnak, F.; Sezgin, H.T.; Uyar, A.; 03.01. Department of Bioengineering; 03.02. Department of Chemical Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThis chapter includes various aspects of antibodies with a major emphasis on computational tools in antibody design. In this scope, current antibody databases, computational tools, and approaches for modelling antibodies and nanobodies, the structure, and interface prediction are analyzed. On the other hand, various antibody design techniques such as grafting, the significance of antibody-drug conjugates, and how computational methods contribute to the development of therapeutic antibodies are discussed with relevant strategies in the literature. Lastly, the production cost and the rising market value of antibodies are emphasized. © IOP Publishing Ltd 2023. All rights reserved.Article Citation - WoS: 1Citation - Scopus: 1Valorization of Black Carrot Pomace and Pea Powder for Co-Production of Polygalacturonase and Pectin Lyase(Springer Science and Business Media Deutschland GmbH, 2025) Uzuner, Sibel; Uzuner, S.; Sezgin, H.T.; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyPectic enzyme groups, particularly polygalacturonase and pectin lyase, are vital components of a high-value microbial enzyme category widely employed in applications within the fruit juice and wine industries. The exploration of alternative carbon and nitrogen sources remains crucial for enhancing enzyme production while reducing costs. This study evaluates the impact of carbon (black carrot pomace) and nitrogen (pea protein) loading on fermentable sugar content, protein content, and enzyme activities during both batch and fed-batch cultivation. Additionally, three distinct valorization techniques—thermal (steam), thermochemical (steam assisted with dilute acid), and microwave-assisted with dilute acid pretreatments—were assessed for their effectiveness in hydrolyzing black carrot pomace as a carbon source. The findings indicate that microwave-assisted dilute acid, coupled with enzymatic saccharification, resulted in the highest fermentable sugar production (0.493 g/g), achieving an 87.3% conversion yield. Pea protein demonstrated more favorable outcomes with the highest polygalacturonase activity (20.50 ± 0.52 U/L) and pectin lyase activity (46.44 ± 3.45 U/L) compared to whey protein and yeast extract used as nitrogen sources. Meanwhile, the highest polygalacturonase and pectin lyase activity, along with the highest total protein content (52.25 ± 0.06 mg/L), was recorded under the same culture conditions, reaching 164.34 ± 2.26 and 188.22 ± 1.72 U/L, respectively, after 72 h, representing approximately 1.18- and 1.34-fold increases from the batch system. Consequently, these results prove that fed-batch cultivation, utilizing black carrot pomace hydrolyzate as a feeding substrate and pea protein as a nitrogen source, significantly increases polygalacturonase and pectin lyase activity compared to batch cultivation. © The Author(s) 2024.