Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Conference Object The Shape and Size Effect of the Diatom Frustule Addition on the Compression Behavior of an Epoxy(Trans Tech Publications, 2018) Zeren, Doğuş; Kesici, Kutsal; Sukatar, Atakan; Güden, MustafaThe effects of the Achnanthes Taeniata and the diatomaceous earth (diatomite) frustules addition on the compressive strength of an epoxy matrix were investigated experimentally. The Achnanthes Taeniata frustules having relatively high length/diameter aspect ratio (2-4) were isolated and cultured in laboratory. While the as-received commercial natural diatomite frustules were non-homogenous in shape and size. The filling epoxy matrix with ~6 wt% of commercial natural diatomite increased the compressive strength from 60 MPa to 67 MPa, while the Achnanthes Taeniata frustules addition increased to 79 MPa. The increased compressive strength and modulus of the the Achnanthes Taeniata frustules filled epoxy was attributed to the higher aspect ratio and relatively strong bonding with the epoxy matrix. The more effective load transfer from the matrix to the Achnanthes Taeniata frustules associated with the enhanced interface bonding was also proved microscopically. The frustules were observed to pull-out on the fracture surface of the Achnanthes Taeniata frustules filled epoxy.Article Citation - WoS: 2Citation - Scopus: 4Protective Effects of Polyamines Against Uv-A and Uv-B Illumination in Physcia Semipinnata Thalli(Institute of Botany and Botanical Garden Jevremovac, 2017) Esmer, Işıl; Tüney, İnci; Ünal Özakça, Dilek; Sukatar, AtakanThe damage to DNA induced by UV-A and UV-B and protective effects of the polyamines putrescine (put), spermidine (spd) and spermine (spm) were investigated on the lichen Physcia semipinnata in the present study. Our results suggest that significant alterations of the photosynthetic quantum yield ratio occurred in response to increased UV-A and UV-B exposure time. The photosynthetic quantum yield ratio gradually decreased in P. semipinnata following exposure to UV-A and UV-B. Physcia semipinnata thalli which were treated with a polyamine in a concentration of 1 mM were not affected by UV-A exposure for 72 h. In the case of UV-B treatment, the protective polyamine dosage was 0.25 mM. We also used the random amplified polymorphic DNA (RAPD) technique to detect DNA damage. The main changes observed in the RAPD profiles, which were obtained using 12 RAPD primers, were the appearance or disappearance of different bands and variation of their intensities. The use of at least three different primers allowed detection of specific band patterns in both UV-A- and UV-B-exposed samples treated with polyamines as compared to untreated ones.Article Citation - WoS: 12Citation - Scopus: 15Morphological and Molecular Identification of Pennate Diatoms Isolated From Urla, Izmir, Coast of the Aegean Sea(TUBITAK, 2013) Kesici, Kutsal; Tüney, İnci; Sukatar, Atakan; Zeren, Doğuş; Güden, MustafaDiatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as ITS (Internal Transcribed Spacer) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated four diatom samples from Coast of Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological features. Then, we amplified ITS regions using conventional polymerase chain reaction (PCR), sequenced the PCR products and analyzed the sequences using bioinformatic tools. Bioinformatic analysis indicated that the isolated species had high sequence similarity to Pseudo-nitzschia sp., Achnanthes sp., Amphora sp. and Cylindrotheca sp. We believe that molecular identification methods enable rapid and more reliable identification of diatom species and are crucial for monitoring harmful algal blooms.