Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Review Citation - WoS: 39Citation - Scopus: 37Engineered Liposomes in Interventional Theranostics of Solid Tumors(American Chemical Society, 2023) Kommineni, Nagavendra; Chaudhari, Ruchita; Conde, Joao; Cecen, Berivan; Chandra, Pranjal; Prasad, Rajendra; Tamburacı, Sedef; 01. Izmir Institute of TechnologyEngineered liposomal nanoparticles have unique characteristicsas cargo carriers in cancer care and therapeutics. Liposomal theranosticshave shown significant progress in preclinical and clinical cancermodels in the past few years. Liposomal hybrid systems have not onlybeen approved by the FDA but have also reached the market level. Nanosizedliposomes are clinically proven systems for delivering multiple therapeuticas well as imaging agents to the target sites in (i) cancer theranosticsof solid tumors, (ii) image-guided therapeutics, and (iii) combinationtherapeutic applications. The choice of diagnostics and therapeuticscan intervene in the theranostics property of the engineered system.However, integrating imaging and therapeutics probes within lipidself-assembly liposome may compromise their overalltheranostics performance. On the other hand, liposomal systems sufferfrom their fragile nature, site-selective tumor targeting, specificbiodistribution and premature leakage of loaded cargo molecules beforereaching the target site. Various engineering approaches, viz., grafting,conjugation, encapsulations, etc., have been investigated to overcomethe aforementioned issues. It has been studied that surface-engineeredliposomes demonstrate better tumor selectivity and improved therapeuticactivity and retention in cells/or solid tumors. It should be notedthat several other parameters like reproducibility, stability, smoothcirculation, toxicity of vital organs, patient compliance, etc. mustbe addressed before using liposomal theranostics agents in solid tumorsor clinical models. Herein, we have reviewed the importance and challengesof liposomal medicines in targeted cancer theranostics with theirpreclinical and clinical progress and a translational overview.Article Citation - WoS: 17Citation - Scopus: 16Development of Cissus Quadrangularis-Loaded Poss-Reinforced Chitosan-Based Bilayer Sponges for Wound Healing Applications: Drug Release and in Vitro Bioactivity(American Chemical Society, 2023) Tıhmınlıoğlu, Funda; Tamburacı, Sedef; Tıhmınlıoğlu, Funda; 03.02. Department of Chemical Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyNowadays, antibiotic-loaded biomaterials have been widelyusedin wound healing applications. However, the use of natural extractshas come into prominence as an alternative to these antimicrobialagents in the recent period. Among natural sources, Cissus quadrangularis (CQ) herbal extract is usedfor treatment of bone and skin diseases in ayurvedic medicine dueto its antibacterial and anti-inflammatory effects. In this study,chitosan-based bilayer wound dressings were fabricated with electrospinningand freeze-drying techniques. CQ extract-loaded chitosan nanofiberswere coated on chitosan/POSS nanocomposite sponges using an electrospinningmethod. The bilayer sponge is designed to treat exudate wounds whilemimicking the layered structure of skin tissue. Bilayer wound dressingswere investigated with regard to the morphology and physical and mechanicalproperties. In addition, CQ release from bilayer wound dressings and in vitro bioactivity studies were performed to determinethe effect of POSS nanoparticles and CQ extract loading on NIH/3T3and HS2 cells. The morphology of nanofibers was investigated withSEM analysis. Physical characteristics of bilayer wound dressingswere determined with FT-IR analysis, swelling study, open porositydetermination, and mechanical test. The antimicrobial activity ofCQ extract released from bilayer sponges was investigated with a discdiffusion method. Bilayer wound dressings' in vitro bioactivity was examined using cytotoxicity determination, woundhealing assay, proliferation, and the secretion of biomarkers forskin tissue regeneration. The nanofiber layer diameter was obtainedin the range of 77.9-97.4 nm. The water vapor permeabilityof the bilayer dressing was obtained as 4021 to 4609 g/m(2)day, as it is in the ideal range for wound repair. The release ofthe CQ extract over 4 days reached 78-80% cumulative release.The release media were found to be antibacterial against Gram-negativeand Gram-positive bacteria. In vitro studies showedthat both CQ extract and POSS incorporation induced cell proliferationas well as wound healing activity and collagen deposition. As a result,CQ-loaded bilayer CHI-POSS nanocomposites were found as a potentialcandidate for wound healing applications.Article Citation - WoS: 11Fabrication of Helix Aspersa Extract Loaded Gradient Scaffold With an Integrated Architecture for Osteochondral Tissue Regeneration: Morphology, Structure, and in Vitro Bioactivity [2](American Chemical Society, 2023) Tamburacı, Sedef; Tıhmınlıoğlu, Funda; Perpelek, Merve; Aydemir, Selma; Baykara, Başak; Havıtçıoğlu, Hasan; Tıhmınlıoğlu, Funda; 03.02. Department of Chemical Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyRegeneration of osteochondral tissue with its layered complex structure and limited self-repair capacity has come into prominence as an application area for biomaterial design. Thus, literature studies have aimed to design multilayered scaffolds using natural polymers to mimic its unique structure. In this study, fabricated scaffolds are composed of transition layers both chemically and morphologically to mimic the gradient structure of osteochondral tissue. The aim of this study is to produce gradient chitosan (CHI) scaffolds with bioactive snail (Helix aspersa) mucus (M) and slime (S) extract and investigate the structures regarding their physicochemical, mechanical, and morphological characteristics as well as in vitro cytocompatibility and bioactivity. Gradient scaffolds (CHI-M and CHI-S) were fabricated via a layer-by-layer freezing and lyophilization technique. Highly porous and continuous 3D structures were obtained and observed with SEM analysis. In addition, scaffolds were physically characterized with water uptake test, micro-CT, mechanical analysis (compression tests), and XRD analysis. In vitro bioactivity of scaffolds was investigated by co-culturing Saos-2 and SW1353 cells on each compartment of gradient scaffolds. Osteogenic activity of Saos-2 cells on extract loaded gradient scaffolds was investigated in terms of ALP secretion, osteocalcin (OC) production, and biomineralization. Chondrogenic bioactivity of SW1353 cells was investigated regarding COMP and GAG production and observed with Alcian Blue staining. Both mucus and slime incorporation in the chitosan matrix increased the osteogenic differentiation of Saos-2 and SW1353 cells in comparison to the pristine matrix. In addition, histological and immunohistological staining was performed to investigate ECM formation on gradient scaffolds. Both characterization and in vitro bioactivity results indicated that CHI-M and CHI-S scaffolds show potential for osteochondral tissue regeneration, mimicking the structure as well as enhancing physical characteristics and bioactivity. © 2023 The Authors. Published by American Chemical Society.Article Citation - WoS: 30Citation - Scopus: 37Production and Characterization of a Novel Bilayer Nanocomposite Scaffold Composed of Chitosan/Si-nhap and Zein/Poss Structures for Osteochondral Tissue Regeneration(American Chemical Society, 2019) Tamburacı, Sedef; Tıhmınlıoğlu, Funda; Çeçen, Berivan; Üstün, Özcan; Ergür, Bekir Uğur; Havıtçıoğlu, Hasan; Tıhmınlıoğlu, Funda; 03.02. Department of Chemical Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyOsteochondral tissue is hard to regenerate after injuries or degenerative diseases. Traditional treatments still have disadvantages, such as donor tissue availability, donor site morbidity, implant loss, and limited durability of prosthetics. Thus, recent studies have focused on tissue engineering strategies to regenerate osteochondral defects with different scaffold designs. Scaffolds have been developed from monolayer structures to bilayer scaffolds to repair the cartilage-bone interface and to support each tissue separately. In this study, Si-substituted nanohydroxyapatite particles (Si-nHap) and silica-based POSS nanocages were used as reinforcements in different polymer layers to mimic a cartilage-bone tissue interface. Chitosan and zein, which are widely used biopolymers, are used as polymer layers to mimic the structure. This study reports the development of a bilayer scaffold produced via fabrication of two different nanocomposite layers with different polymer-inorganic composites in order to satisfy the complex and diverse regenerative requirements of osteochondral tissue. The chitosan/Si-nHap microporous layer and the zein/POSS nanofiber layer were designed to mimic a bone-cartilage tissue interface. Bilayer scaffolds were characterized with SEM, compression, swelling, and biodegradation tests to determine morphological, physical, and mechanical properties. The results showed that the bilayer scaffold had a structure composed of microporous and nanofiber layers joined at a continuous interface with appropriate mechanical properties. Furthermore, in vitro cell culture studies have been performed with LDH, proliferation, fluorescence imaging, and ALP activity assays using osteosarcoma and chondrosarcoma cell lines. ALP expression levels provide a good illustration of the improved osteogenic potential of a porous chitosan/Si-nHap layer due to the Si-doped nHap incorporation. Histological data showed that both fiber and porous layers that mimic the cartilage and bone sections exhibit homogeneous cell distribution and matrix formation. Histochemical staining was used to determine the cell proliferation and ECM formation on each layer. In vitro studies indicated that zein-POSS/chitosan/Si-nHap nanocomposite bilayer scaffolds showed promising results for osteochondral regeneration. Copyright © 2019 American Chemical Society.