Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Conference Object Citation - Scopus: 1Colorimetric Detection of Creatinine on an Electromechanical Mixing Platform(IEEE, 2021) Tarim, E. Alperay; Oksuz, Cemre; Karakuzu, Betul; Tekin, H. CumhurWe present an electromechanical mixing platform integrated with a smartphone for colorimetric detection of creatinine using an enzymatic reaction between horseradish peroxidase (HRP) and 3,3',5,5'-Tetramethylbenzidine (TMB). On the developed platform, the color resulting from the reaction between HRP and TMB in a reservoir of a poly(methyl methacrylate) (PMMA) chip is measured using a smartphone camera. With mixing, diluted creatine solutions can be detected in 1 min that can reduce long waiting times of creatinine detection due to enzymatic reactions. Furthermore, smartphone-based colorimetric detection reduces the cost of analysis by eliminating costly equipment for spectrometric measurements without affecting the sensitivity of analysis. We, therefore, think that the presented platform integrated with a smartphone could be used for automatic measurement of creatinine level in the sample by allowing low-cost and rapid analysis, and this would be particularly beneficial for monitoring of chronic kidney disease (CKD) at the point-of-care setting.Review Citation - WoS: 7Citation - Scopus: 8Magnetic Levitation-Based Miniaturized Technologies for Advanced Diagnostics(Springernature, 2024) Karakuzu, Betul; Inevi, Muge Anil; Tarim, E. Alperay; Sarigil, Oyku; Guzelgulgen, Meltem; Kecili, Seren; Tekin, H. CumhurTaking advantage of the magnetic gradients created using magnetic attraction and repulsion in miniaturized systems, magnetic levitation (MagLev) technology offers a unique capability to levitate, orient and spatially manipulate objects, including biological samples. MagLev systems that depend on the inherent diamagnetic properties of biological samples provide a rapid and label-free operation that can levitate objects based on their density. Density-based cellular and protein analysis based on levitation profiles holds important potential for medical diagnostics, as growing evidence categorizes density as an important variable to distinguish between healthy and disease states. The parallel processing capabilities of MagLev-based diagnostic systems and their integration with automated tools accelerates the collection of biological data. They also offer notable advantages over current diagnostic techniques that require costly and labor-intensive protocols, which may not be accessible in a low-resource setting. MagLev-based diagnostic systems are user-friendly, portable, and affordable, making remote and label-free applications possible. This review describes the recent progress in the application of MagLev principles to existing problems in the field of diagnostics and how they help discover the molecular- and cellular-level changes that accompany the disease or condition of interest. The critical parameters associated with MagLev-based diagnostic systems such as magnetic medium, magnets, sample holders, and imaging systems are discussed. The challenges and barriers that currently limit the clinical implications of MagLev-based diagnostic systems are outlined together with the potential solutions and future directions including the development of compact microfluidic systems and hybrid systems by leveraging the power of deep learning and artificial intelligence.Article Citation - WoS: 7Citation - Scopus: 7Colorimetric Detection of Serum Creatinine on a Miniaturized Platform Using Hue-Saturation Space Analysis(Nature Portfolio, 2024) Tarim, E. Alperay; Tekin, H. CumhurChronic kidney disease (CKD) is a widespread condition with considerable health and economic impacts globally. However, existing methodologies for serum creatinine assessment often involve prolonged wait times and sophisticated equipment, such as spectrometers, hindering real-time diagnosis and care. Innovative solutions like point-of-care (POC) devices are emerging to address these challenges. In this context, there is a recognized need for remote, regular, automated, and low-cost analysis of serum creatinine levels, given its role as a critical parameter for CKD diagnosis and management. This study introduces a miniaturized system with integrated heater elements designed for precise serum creatinine measurement. The system operates based on the Jaffe method and accurate serum creatinine measurement within a microreservoir chip. Smartphone-based image processing using the hue-saturation-value (HSV) color space was applied to captured images of microreservoirs. The creatinine analyses were conducted in serum with a limit of detection of similar to 0.4 mg/dL and limit of quantification of similar to 1.3 mg/dL. Smartphone-based image processing employing the HSV color space outperformed spectrometric analysis for creatinine measurement conducted in serum. This pioneering technology and smartphone-based processing offer the potential for decentralized renal function testing, which could significantly contribute to improved patient care. The miniaturized system offers a low-cost alternative ($87 per device), potentially reducing healthcare expenditures (similar to $0.5 per test) associated with CKD diagnosis and management. This innovation could greatly improve access to diagnosis and monitoring of CKD, especially in regions where access to sophisticated laboratory equipment is limited.