Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 4Citation - Scopus: 4Surface Modification Via Alkali Treatment and Its Effect on the Physicochemical and Biological Properties of Emulsion Templated Scaffolds(Elsevier Sci Ltd, 2025) Kocagoz, Mehmet; Tihminlioglu, Funda; Dikici, Betul AldemirEmulsion templating is an advantageous scaffold fabrication technique that provides high interconnectivity, high porosity, and control of the scaffold architecture. Polymerised emulsions with an internal phase ratio greater than 74 % are named Polymerised High Internal Phase Emulsions (PolyHIPEs). Polycaprolactone (PCL) is a synthetic, biodegradable, and biocompatible polymer widely used in tissue engineering, but the material-cell interaction of PCL-based biomaterials has been found to be limited due to the material's high hydrophobicity. This study aims to develop emulsion-templated polycaprolactone tetramethacrylate (4PCLMA)-based scaffolds and improve their biological performance using an alkaline surface modification method. For this purpose, 4PCLMA was successfully synthesised, and highly porous scaffolds were developed. PolyHIPEs were incubated in three different sodium hydroxide (NaOH) concentrations for three different incubation times. Chemical, morphological, mechanical characterisation, mass loss, water absorption capacity, water contact angle, Brunauer-Emmett-Teller analyses and biological investigations were conducted on NaOH-treated scaffolds in comparison with the control. The chemical changes induced by NaOH treatment in PolyHIPEs were confirmed by Fourier-transform infrared spectroscopy. NaOH treatment increased the water absorption capacity, hydrophilicity, surface area, and protein adsorption but decreased the weight and mechanical strength of the scaffolds. In vitro results showed that NaOH treatment did not cause cytotoxicity in L929 cells and positively affected the cell adhesion and proliferation behaviour of Saos-2 cells. This study suggests surface modification of biodegradable synthetic polymer-based PolyHIPEs by NaOH treatment as a simple, scalable and cost-effective approach to enhance cell-material interactions of the material without causing a significant change in the overall morphology, contributing to the advancement of next-generation healthcare technologies.Article Citation - WoS: 2Citation - Scopus: 2Fabrication of Bioactive Helix Aspersa Extract-Loaded Chitosan-Based Bilayer Wound Dressings for Skin Tissue Regeneration(Amer Chemical Soc, 2024) Perpelek, Merve; Tıhmınlıoğlu, Funda; Tamburaci, Sedef; Karakasli, Ahmet; Tihminlioglu, FundaIn recent years, there has been a notable shift toward exploring plant and animal extracts for the fabrication of tissue engineering structures that seamlessly integrate with the human body, providing both biological compatibility and physical reinforcement. In this particular investigation, we synthesized bilayer wound dressings by incorporating snail (Helix aspersa) secretions, comprising mucus and slime, into chitosan matrices via lyophilization and electrospinning methodologies. A nanofiber layer was integrated on top of the porous structure to mimic the epidermal layer for keratinocyte activity as well as acting as an antibacterial barrier against possible infection, whereas a porous structure was designed to mimic the dermal microenvironment for fibroblast activity. Comprehensive assessments encompassing physical characterization, antimicrobial efficacy, in vitro bioactivity, and wound healing potential were conducted on these bilayer dressings. Our findings revealed that the mucus and slime extract loading significantly altered the morphology in terms of nanofiber diameter and average pore size. Snail extracts loaded on a nanofiber layer of bilayer dressings showed slight antimicrobial activity against Staphylococcus epidermidis and Escherichia coli. An in vitro release study of slime extract loaded in the nanofiber layer indicated that both groups 1 and 2 showed a burst release up to 6 h, and a sustained release was observed up to 96 h for group 1, whereas slime extract release from group 2 continued up to 72 h. In vitro bioactivity assays unveiled the favorable impact of mucus and slime extracts on NIH/3T3 fibroblast and HS2 keratinocyte cell attachment, proliferation, and glycosaminoglycan synthesis. Furthermore, our investigations utilizing the in vitro scratch assay showcased the proliferative and migratory effects of mucus and slime extracts on skin cells. Collectively, our results underscore the promising prospects of bioactive snail secretion-loaded chitosan constructs for facilitating skin regeneration and advancing wound healing therapies.Article Citation - WoS: 14Citation - Scopus: 163D Bioprinting of mouse pre-osteoblasts and human MSCs using bioinks consisting of gelatin and decellularized bone particles(Iop Publishing Ltd, 2024) Kara, Aylin; Distler, Thomas; Akkineni, Ashwini Rahul; Tihminlioglu, Funda; Gelinsky, Michael; Boccaccini, Aldo R.One of the key challenges in biofabrication applications is to obtain bioinks that provide a balance between printability, shape fidelity, cell viability, and tissue maturation. Decellularization methods allow the extraction of natural extracellular matrix, preserving tissue-specific matrix proteins. However, the critical challenge in bone decellularization is to preserve both organic (collagen, proteoglycans) and inorganic components (hydroxyapatite) to maintain the natural composition and functionality of bone. Besides, there is a need to investigate the effects of decellularized bone (DB) particles as a tissue-based additive in bioink formulation to develop functional bioinks. Here we evaluated the effect of incorporating DB particles of different sizes (<= 45 and <= 100 mu m) and concentrations (1%, 5%, 10% (wt %)) into bioink formulations containing gelatin (GEL) and pre-osteoblasts (MC3T3-E1) or human mesenchymal stem cells (hTERT-MSCs). In addition, we propose a minimalistic bioink formulation using GEL, DB particles and cells with an easy preparation process resulting in a high cell viability. The printability properties of the inks were evaluated. Additionally, rheological properties were determined with shear thinning and thixotropy tests. The bioprinted constructs were cultured for 28 days. The viability, proliferation, and osteogenic differentiation capacity of cells were evaluated using biochemical assays and fluorescence microscopy. The incorporation of DB particles enhanced cell proliferation and osteogenic differentiation capacity which might be due to the natural collagen and hydroxyapatite content of DB particles. Alkaline phosphatase activity is increased significantly by using DB particles, notably, without an osteogenic induction of the cells. Moreover, fluorescence images display pronounced cell-material interaction and cell attachment inside the constructs. With these promising results, the present minimalistic bioink formulation is envisioned as a potential candidate for bone tissue engineering as a clinically translatable material with straightforward preparation and high cell activity.