Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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  • Article
    Citation - WoS: 4
    Citation - Scopus: 4
    Identification of Volatile Biomarkers in Exhaled Breath by Polythiophene Solid Phase Microextraction Fiber for Disease Diagnosis Using Gc-Ms
    (Elsevier, 2024) Pelit, Fusun; Goksel, Ozlem; Dizdas, Tugberk Nail; Arin, Aycan; Ozgur, Su; Erbas, Ilknur; Pelit, Levent
    The diagnosis of diseases through monitoring of volatile organic compounds (VOCs) in exhaled breath (EB) holds great potential for clinical applications. However, a standardized method for VOC analysis in EB yet to be proposed. The present study presents an untargeted method for screening and identifying potential volatile biomarkers in EB by a lab-made solid phase microextraction (SPME) fiber. A polythiophene-based SPME fiber was produced by an electrochemical method and VOC sampling was performed under dynamic and controlled conditions. Following the sampling step, the adsorbed VOCs on the SPME fiber were analyzed using gas chromatography-mass spectrometry (GC-MS). The VOCs in EB were screened by the MS detector in selected ion monitoring (SIM) mode within the mass/charge (m/z) range of 13-94 values. Potential biomarkers among all detected VOCs in each subject's EB sample were identified through machine learning algorithms, employing a comparative analysis of distinctive retention times (RT) and peak areas between the lung cancer (LC) and control groups in two stages. In the initial stage of the study, the areas of all peaks observed in the SIM-GC-MS chromatograms of 25 LC and 51 control group subjects were integrated, and the resulting retention times and peak areas were recorded for subsequent analysis to identify potential biomarkers. A total of 1.346 distinct compounds were detected among the 76 subjects in this step, and statistical analysis using the LightGBM algorithm revealed the potential biomarkers for LC diagnosis. The PTh-SPME fibre successfully identified four novel cancer biomarkers in breath matrix: 4-heptenal, 4-methyl-1-octene, 1,2,3,4-tetrahydro-5,8-dimethyl-1-octylnaphthalene and tetrahydro-2-(2,5-undecadiynyloxy)-2H-pyran. In the second step of the study, the efficacy of the top ten selected biomarkers was evaluated in a cohort of 166 subjects, including 70 individuals with LC and 96 in the control group. The model achieved accuracy, area under the curve (AUC), and F Score values of 0.818, 0.816, and 0.817, respectively. The test model correctly predicted 27 out of 33 subjects between LC and control groups.
  • Article
    Citation - WoS: 6
    Citation - Scopus: 7
    A Comprehensive Study on Doxorubicin-Loaded Aspartic Acid-Coated Magnetic Fe<sub>3</Sub>o<sub>4< Nanoparticles: Synthesis, Characterization and in Vitro Anticancer Investigations
    (Elsevier, 2024) Jafari, Nahideh; Mohammadpourfard, Mousa; Hamishehkar, Hamed
    Magnetic Fe3O4 nanoparticles (MNPs) hold significant potential across various scientific fields due to their notable properties. For biomedical applications, MNPs must be biocompatible, stable, and possess high magnetic potential. Aspartic acid (ASP) as a coating agent not only provides biocompatibility, stability, and high magnetic potential but also offers the potential for absorbing various drugs for targeted delivery due to its carboxyl and amino functional groups. So, in this study, we synthesized ASP-coated MNPs (ASP-MNPs) through a one-step co-precipitation method and loaded doxorubicin (DOX) onto these nanoparticles to create DOX-ASP-MNPs for targeted drug delivery. Characterization of the nanoparticle confirmed the crystal structure, spherical morphology, and improved size distribution of ASP-MNPs (8.53 +/- 2.56 nm) compared to uncoated MNPs (7.05 +/- 1.89 nm), as analyzed by XRD, FESEM, and TEM. FT-IR and zeta potential assessments (ZP = -6.3 mV for MNPs, ZP = -31.1 mV for ASP-MNPs) verified successful ASP binding, DOX loading, and nanoparticle stability. VSM analysis indicated a slight decrease in saturation magnetism after coating (51.1 emu/g) compared to MNPs (57.4 emu/g). In vitro release studies demonstrated a higher release rate (83 %) of DOX-ASP-MNPs at pH 5.2, indicating their suitability for cancerous cells. Cytotoxicity assays on A-549 cancer cell lines showed a dose-dependent response. DAPI staining revealed that free DOX caused more DNA damage. Cellular uptake studies indicated a time-dependent uptake of DOX-ASP-MNPs, higher at 3 h compared to 1 h, though lower than free DOX uptake due to different uptake pathways. Apoptosis assays over 72 h showed similar apoptotic rates for DOX-ASP-MNPs and free DOX. These findings suggest that ASP-MNPs possess enhanced physicochemical properties and effective drug delivery capabilities, making them a promising candidate for different biomedical applications, particularly targeted cancer therapy.