Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

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Department: search.filters.department.İzmir Institute of TechnologySubject: search.filters.subject.Microalgae

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  • Article
    Production of Microalgae-Derived Exo-Polygalacturonase for Fruit Juice Clarification
    (Frontiers Media S.A., 2022) Uzuner, Sibel; Evrendilek, Gulsun Akdemir; Kurhan, Sebnem
    Due to the growing demand for commercial enzymes derived from renewable and sustainable resources with higher efficacy, algal biomass has gained more attention. Thus, the production of exo-polygalacturonase (PGase) from Bacillus subtilis under submerged fermentation (SmF) using algal feedstocks as a carbon source in fermentation medium and its potential use for the clarification of apple and carrot juices with the optimization of the enzyme loading, pH, and time using Box-Behnken response surface methodology (RSM) were prompted. The maximum PGase activity of 510.61 +/- 7.86 U/L was achieved at pH 5.0 after 48 h at 40 degrees C using 0.5% (w/v) pectin, 0.1% (w/v) yeast extract, 0.02% (w/v) MgSO47H2O, 0.02% (w/v) K2HPO4, 0.3% (w/v) glucose, and 0.583% (w/v) microalgae hydrolysate as a fermentation medium. PGase production without glucose was favored by SmF with a shake flask working volume of 100 ml (248.90 +/- 4.58 U/L) as compared to a bioreactor with a working volume of 1 L (192.99 +/- 0.84) for 48 h. Both carrot and apple juices were treated with the crude PGase (248.9 U/L) at different concentrations (0.1-0.5%), temperature (30-50 degrees C), and time (30-120 min), and the maximal clarification conditions were obtained as 0.3% (w/v) enzyme concentration at 50 degrees C for 30 min exposure time. This study revealed that microalgae-derived PGase in crude form could be effectively used for clarification of the juices.