Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Book Part Sample Preparation Using Microfluidic Technologies for Non-Invasive Tests(Elsevier, 2025) Oksuz, C.; Tarim, E.A.; Ozcan, H.A.; Koc, S.; Tekin, H.C.The collection of a biological sample and the steps carried out to obtain the target in a sample covers the sample preparation procedures which are one of the important steps for diagnostic tests. Removing interferences in a complex sample, preventing undesirable reactions, separating, purifying, and enriching the sample are among the steps that can be applied to samples for analysis. Non-invasive tests include samples such as urine, saliva, sweat, tear, breath and are preferred because they are simple, painless, cost-effective and cause fewer complications. In traditional methods applied in clinics, most of the steps such as centrifugation, pipetting, staining, and washing are performed manually by a technician. For this reason, tests are costly, require long analysis time, and have a significant risk of contamination and manual errors. Microfluidic technologies allow automating sample collection and preparation steps by integrating many components on a single chip. Thereby, low-volume samples can be processed automatically with high efficiency and purity. In this chapter, the sample preparation methods used in microfluidic devices for non-invasive tests analyzing human samples including sweat, urine, saliva, tears, sexual samples, and other body fluids are reviewed. This information aims to facilitate the development of potential sample preparation methods and applications for non-invasive diagnostic tests. © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.Conference Object Serum Creatinine Detection in a Microfluidic Chip Using a Smartphone Camera(Chemical and Biological Microsystems Society, 2022) Karakuzu, B.; Tarim, E.A.; Tekin, H.C.We present a microfluidic chip platform to detect serum creatinine levels using the enzyme-linked immunosorbent assay (ELISA) principle. In the platform, surface modified microfluidic channel sensitively captured target molecules from the serum sample, and then ELISA protocol was applied inside the channels. Afterward, the blue color formed as a result of the enzymatic reaction was measured via a smartphone camera. The proposed strategy allows the detection of creatinine rapidly in a minute amount of the serum samples without the need for expensive equipment. Thus, chronic kidney disease (CKD) could be monitored easily at point-of-care settings via the proposed creatinine detection strategy. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.Article Introducing Engineering Students To Microfluidics and 3d Printing Using Hands-On Activities(American Society for Engineering Education, 2023) Dogan, E.; Borgaonkar, A.D.; Nafisi, N.; Miri, A.K.Microfluidics technology involves the regulation of flow in micron-sized channels for desired reactions, with applications in biological modeling, drug manufacturing, screening of biological agents, and various engineering fluid dynamics-related purposes. Despite its growth and development, microfluidics has not been widely included as a teaching topic in undergraduate engineering education. This manuscript presents a hands-on project-based learning approach that can be easily implemented into core engineering courses, such as fluid mechanics, transport, chemical reactions, and others. Project-based activities presented here have three main parts: material preparation based on synthetic polymers, light-assisted manufacturing of a microfluidic device, and mass transport experiments to observe the fluid behavior. The project leverages 3D printing and the potential to connect students with makerspaces and 3D printing and to get them started on the path to bringing their ideas to life. The paper includes a breakdown of how to access and evaluate these activities. As a result of this hands-on activity, students will understand how fluid mechanics concepts are applied to microfluidics. Students will also learn about a novel interdisciplinary field that is growing rapidly. Engineering technology students will benefit from exposure to the application side of this emerging field through these lab-style activities that they are accustomed to in the majority of their core courses. Finally, the authors hope that such successful integration will encourage faculty to introduce other novel science and engineering topics that are currently only accessible through research experiencebased courses. © 2023, American Society for Engineering Education. All rights reserved.