Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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Scopus Q: search.filters.scopusQuartile.Q1Subject: search.filters.subject.Xylanase

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  • Article
    Citation - WoS: 19
    Citation - Scopus: 20
    Processing of Hazelnut (corylus Avellana L.) Shell Autohydrolysis Liquor for Production of Low Molecular Weight Xylooligosaccharides by Aureobasidium Pullulans Nrrl Y-2311 Xylanase
    (Elsevier, 2021) Sürek, Ece; Büyükkileci, Ali Oğuz; Yeğin, Sırma
    In this study, a versatile process for the production of xylooligosaccharides (XOS) with a low degree of polymerization (DP 2-6) from hazelnut shells was designed. This process included autohydrolysis integrated with sequential enzymatic hydrolysis by crude xylanase produced with Aureobasidium pullulans NRRL Y-2311-1 from wheat bran. Autohydrolysis of hazelnut shells was carried out at a solid:liquid ratio of 1:6 (w/w) and 190 degrees C nonisothermally. The effects of several parameters on enzymatic hydrolysis of the autohydrolysis liquor were determined. The maximum XOS (DP 2-6) production was 22.5 g/L which was obtained at pH 5.0 and 40 degrees C using enzyme concentration of 240 U/g XOS and substrate concentration of 72 g/L. Under these conditions, 31.29 % of the substrate (total XOS) was converted to low-DP-XOS; xylobiose and xylotriose are being the major oligomers. This is the first study on the application of A. pullulans xylanase in production of xylooligomers from hazelnut shells.
  • Article
    Citation - WoS: 16
    Citation - Scopus: 18
    A Novel Thermostable Xylanase From Geobacillus Vulcani Gs90: Production, Biochemical Characterization, and Its Comparative Application in Fruit Juice Enrichment
    (Wiley, 2021) Algan, Müge; Sürmeli, Yusuf; Şanlı Mohamed, Gülşah
    Xylanases have great attention to act as a potential role in agro-industrial processes. In this study, production, characterization, and fruit juice application of novel xylanase from thermophilic Geobacillus vulcani GS90 (GvXyl) were performed. GvXyl was purified via acetone precipitation and gel-filtration chromatography. The results showed that GvXyl had 1,671.4 U/mg of specific activity and optimally worked at pH 8 and 55 degrees C. It was also active in a wide pH (3-9) and temperature (30-90oC) ranges. GvXyl was highly stable at 90oC and relatively stable at pH 3-9. The kinetic parameters of GvXyl were obtained as K-m, V-max, and k(cat); 10.2 mg/ml, 4,104 mu mol min(-1) mg(-1), and 3,542.6 s(-1), respectively. GvXyl had higher action than commercial xylanase in fruit juice enrichment. These results revealed that GvXyl might possess a potential influence in fruit juice processing because of its high specific activity and great thermal stability. Practical applications Polysaccharides include starch, pectin, and hemicellulose create problems by lowering fruit juice quality in beverages. To overcome this problem, various clarification processes might be applied to natural fruit juices. Even though chemicals are widely used for this purpose, recently enzymes including xylanases are preferred for obtaining high-quality products. In this study, we reported the production and biochemical characterization of novel thermostable xylanase from thermophilic G. vulcani GS90 (GvXyl). Also, apple and orange juice enrichment were performed with the novel xylanase to increase the quality in terms of yield, clarity, and reducing sugar substance. The improved quality features of apple and orange juices with GvXyl was then compared to commercially available beta-1,4-xylanase. The results revealed that GvXyl might possess a potential influence in fruit juice processing because of its high specific activity and great thermal stability.