Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Developing Gold Nanoparticles Decorated With Carbon-Dots for Multiplexed Cellular Imaging(IOP Publishing Ltd, 2025) Kavuranpala, Tugce; Saydullaeva, Iroda; Ozcelik, SerdarThis study focuses on developing a novel hybrid nanomaterial composed of gold nanoparticle decorated with carbon dots, termed AuNP@C-dot, as a versatile platform for multiplexed imaging. Structural and spectral characterizations confirmed the successful conjugation of C-dots to AuNPs via covalent bonding, as evidenced by FTIR, X-ray photoelectron spectra, HRTEM analyses, and UV-Vis and fluorescence spectroscopies. The fluorescence intensities of C-dots are doubled through the conjugation to the AuNPs. The conjugation of fluorescent C-dots to plasmon-resonant AuNPs enables simultaneous multicellular imaging by taking advantage of the fluorescent signaling of C-dots and the scattering signaling of AuNPs. In vitro studies using human lung cell lines (A549 and BEAS-2B) confirmed the multiplexed imaging and revealed efficient cellular uptake and subcellular localization of AuNP@C-dots, including nuclear translocation, which is critical for radiotherapy and photodynamic therapy. Cell viability assessments utilizing a colorimetric assay for measuring cell metabolic activity and a colony formation assay demonstrated good biocompatibility of AuNP@C-dots at relevant concentrations. It can be envisioned that the AuNP@C-dot hybrid system may improve the detection and monitoring of cell health and disease due to its dual-modal imaging capability. Furthermore, they could be used for supervising controlled release of therapeutic agents, tailored for enhanced treatment efficacy. This study demonstrates the potential of C-dot-conjugated AuNPs as a multifunctional tool with inherent control mechanisms for the next-generation cellular analysis, drug administration, and diagnostic strategies.Conference Paper Magnetic Levitation-Based Endothelial Cell Sorting(Institute of Electrical and Electronics Engineers Inc., 2023) Ozcan, H.A.; Kecili, S.; Tekin, H.C.Cell sorting for rare cells is crucial for diagnostic purposes. Circulating Endothelial Cells (CECs) can be used as cardiovascular disease markers. Due to the rareness of the CECs in the blood, an accurate, easy, cost and time-effective sorting method is a need. Magnetic levitation is a promising technique for observing differences in the average height of endothelial and white blood cells which does not require any labeling. This study aims to show that the magnetic levitation principle can be used for sorting endothelial cells from the blood. By using paramagnetic medium concentrations of 10 mM and 50 mM, the average levitation height between HUVECs used as a model endothelial cells and U937 cells used as model of white blood cells was measured as 65 μm and 32 μm, respectively. Since there is a significant difference in levitation height for HUVECs and U937 cells, magnetic levitation technology exhibits promising potential for the precise sorting of endothelial cells. © 2023 IEEE.Article Citation - WoS: 14Citation - Scopus: 18Separating Normosmic and Anosmic Patients Based on Entropy Evaluation of Olfactory Event-Related Potentials(Elsevier Ltd., 2019) Güdücü, Çağdaş; Olcay, Bilal Orkan; Schaefer, L.; Aziz, M.; Schriever, V. A.; Özgören, Murat; Hummel, T.Objective: Methods based on electroencephalography (EEG) are used to evaluate brain responses to odors which is challenging due to the relatively low signal-to-noise ratio. This is especially difficult in patients with olfactory loss. In the present study, we aim to establish a method to separate functionally anosmic and normosmic individuals by means of recordings of olfactory event-related potentials (OERP) using an automated tool. Therefore, Shannon entropy was adopted to examine the complexity of the averaged electrophysiological responses. Methods: A total of 102 participants received 60 rose-like odorous stimuli at an inter-stimulus interval of 10 s. Olfactory-related brain activity was investigated within three time-windows of equal length; pre-, during-, and post-stimulus. Results: Based on entropy analysis, patients were correctly diagnosed for anosmia with a 75% success rate. Conclusion: This novel approach can be expected to help clinicians to identify patients with anosmia or patients with early symptoms of neurodegenerative disorders. Significance: There is no automated diagnostic tool for anosmic and normosmic patients using OERP. However, detectability of OERP in patients with functional anosmia has been reported to be in the range of 50%.