Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Validation of New Micro-Spectrophotometric Method To Determine Diamine Oxidase Activity in Serum(Springer india, 2024) Sozmen, Eser Yildirim; Sozmen, Alper BaranFood allergy and histamine intolerance (HI) are prevalent worldwide. Diamine oxidase (DAO) activity determination in serum is a promising approach for diagnosing HI. Despite the numerous methods that have been published for determining diamine. oxidase activity in serum, most of them have not been validated and have inherent limitations, such as interference and multi-step analysis. Our objective was to perform the analytical validation of a novel micro-spectrophotometric method for the determination of DAO activity in a routine clinical laboratory setting. In this study we determined DAO activity in serum using a 4-aminoantypirine (4-AAP) and 2,4,6 tribromo-3-hidroxybenzoic acid (TBHBA) combination as chromogen for the first time. Validation steps included determination of accuracy (recovery and linearity) and precision (within-run and between-run repeatability) following the Clinical and Laboratory Standards Institute protocols and the other guidelines. Reagent stability assessment at both 4 degrees C temperature and frozen (- 20 degrees C) after 4 h, 24 h, 72 h and a week storage were conducted. The optimum test conditions were determined as pH = 6.0, 37 degrees C, 55 mM Putrescine and 60 min. LOD and LOQ levels were found as 10.08 U/L and 30.56 U/L respectively. Coefficients of variation (CVs) for within-run and between-run precisions were 5.2%, 9.4% for the normal level and 2.6%, 2.2% for the high range, respectively. This reliable method for quantifying DAO activity in serum provides an optimal approach, offering rapid and straightforward performance in laboratories for clinical use.Article Citation - WoS: 1Citation - Scopus: 1Exploring the Heterogeneity of Ige-Mediated Food Allergy Through Latent Class Analysis(S. Karger AG, 2022) Akarsu, Ayşegül; Öksel Karakuş, Ceyda; Ocak, Melike; Oral, Nihan; Bilgi, Eyüp; Şahiner, Ümit Murat; Soyer, Özge; Şekerel, Bülent EnisIntroduction: Food allergy (FA) is a heterogeneous disease with multiple morbidities and a huge burden for patients and healthcare systems. Variable manifestations, comorbidities (atopic dermatitis [AD], asthma, and/or allergic rhinitis [AR]), severity (anaphylaxis), and outcomes suggest the existence of different endotypes that cluster analyses may reveal. In this study, we aimed to investigate distinct subgroups among patients with FAs using data from 524 children/adolescents. Methods: 524 patients with IgE-mediated FA (353 male [67%]; median age 4.4 years [IQR:3.0-6.8]), 354 (68%) had multiple FA. The history of AD, asthma, AR, and anaphylaxis was recorded in 59.4%, 35.5%, 24.2%, and 51.2% of the patients, respectively. Latent class analysis was carried out to distinguish clinical FA phenotypes using five potential markers of allergy severity (single/multiple FA, never/inactive/current asthma and AD, AR, and anaphylaxis). Results: Three distinct phenotypes were identified: (1) multiple FA with eczema and respiratory multimorbidity (42%), (2) multiple FA with persistent eczema (34%), and (3) single FA with respiratory multimorbidity without eczema (24%). Compared with the single FA cluster, the prevalence of AD was significantly higher in multiple FA groups. Cluster 1 had the highest frequency of AR and allergic asthma, and the lowest rate of total tolerance of FA. Discussion: We put forward the hypothesis of underlying pathogenesis according to the clinical phenotypes. While skin barrier defect may play a dominant role in the pathogenesis in Cluster 2, immune dysregulation may be dominant in Cluster 3. In Cluster 1, the most severe group, a combination of both skin barrier defects and immune dysregulation may be responsible for the clinical features.