Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 2Citation - Scopus: 1Dynamics of Co2 Consumption, and Biomass and Lipid Carbon Production During Photobioreactor Cultivation of the Diatom Cyclotella(TÜBİTAK - Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, 2023) Ökten, HaticeUnderstanding of CO2 delivery and consumption dynamics in algal photobioreactors are critical to unravel microalgae’s full potential for bioproduct generation and carbon capture from flue gas streams. This study aims to expand our current understanding by cultivating the diatom Cyclotella under controlled process conditions of a bubble column photobioreactor and analyzing CO2 consumption dynamics in real time using results from an online CO2 sensor connected to the reactor exhaust. Two sets of experiments were conducted: they served to contrast the influence of silicon and nitrate (Si&N colimitation) and Si limitation, and the light availability, respectively. CO2 consumption was calculated based on the mass balance around the reactor inlet and outlet gas streams. Biomass samples and lipid extracts were analyzed for carbon (C) content to determine biomass-C and lipid-C concentrations. The outlet CO2 concentrations varied significantly with cultivation time and process conditions. More than 15% to 65% of the CO2 introduced left the reactor in the exhaust at any instance based on the set CO2 transfer rates. The highest average daily capturing efficiency was 60%. Nutrient limitation regimes imposed generated unique CO2 consumption profiles undiscernible by the biomass-C analysis, i.e. unlike Si limitation, N limitation had more immediate detrimental effects on C consumption. Final biomass-C concentration increased with increasing N and light availability, 275 mg/L vs. 336 mg/L, and 270 mg/L vs. 501 mg/L, respectively. Biomass-C based capturing efficiency approximations resulted in 20% to 40% underestimation. Under Si-limited conditions, the higher light intensity increased the final lipid-C to biomass-C ratio by two times (from 20% to 40%) and the final lipid-C concentration and peak productivity by four times (from 56 mg/L to 216 mg/L, from 7 to 30 mg/L-day, respectively). This study demonstrates online exhaust CO2 concentration-based analysis’s unique capabilities for assessing carbon availability and capture, organic-C production, and its diversion to biomass and lipid production.Article Citation - WoS: 3Citation - Scopus: 4Comparison of the Effects of Statins on A549 Nonsmall-Cell Lung Cancer Cell Line Lipids Using Fourier Transform Infrared Spectroscopy: Rosuvastatin Stands Out(Wiley, 2021) Aksoy, Hatice Nurdan; Ceylan, ÇağatayStatins are commonly prescribed antilipidemic and anticholesterol class of drugs. In addition to their major role, they have been found to have anticancer effects on in vitro, animal and clinical studies. The aim of this study was to investigate the effects of six different statins (rosuvastatin, pravastatin, simvastatin, lovastatin, fluvastatin, and atorvastatin) on A549 cancer cells lipids by Fourier transform infrared (FTIR) spectroscopy. Proliferation tests were carried out to detect the half-maximal inhibitory concentrations (IC50) of each statin on A549 cells. The IC50 values were 50 mu M for simvastatin, 150 mu M for atorvastatin and pravastatin, and 170 mu M for fluvastatin, 200 mu M for rosuvastatin and lovastatin on A549 cells. No correlation was found between the antiproliferative effects of the statins and lipid-lowering effect. The cells were treated with IC5, IC10, and IC50 values of each statins concentration and lipid extracts were compared using FTIR spectroscopy. The results indicated that different statins had different effects on the lipid content of A549 cells. The FTIR spectra of the lipid exctracts of statin-treated A549 cells indicated that the value of hydrocarbon chain length, unsaturation index, oxidative stress level, and phospholipid containing lipids increased except for rosuvastatin-treated A549 cells. In addition, rosuvastatin significantly lowered cholesterol ester levels. In conclusion, the contrasting effects of rosuvastatin should be further investigated.