Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
Browse
Search Results
Article Citation - WoS: 2Citation - Scopus: 2Synthesis of Oleic Acid - Coated Zinc - Doped Iron Boride Nanoparticles for Biomedical Applications(Elsevier Sci Ltd, 2024) Paksoy, Aybike; Aydemir, Duygu; Somer, Mehmet; Ulusu, Nuriye Nuray; Balci-Cagiran, OzgeAlthough various iron-based magnetic materials have been extensively studied in biomedical field for many years, iron boride compounds with interesting chemical and magnetic properties are relatively less explored, and their potential applications are not as widely known. In this study, the synthesis, coating, surface modification, and cytotoxicity tests of the Fe-Zn-B system were presented. Iron boride-based nanoparticles (NPs) containing elemental zinc (Zn) were developed by using a direct chemical synthesis of FeCl3, 3 , ZnCl2 2 and NaBH4, 4 , and investigated for potential use in biomedical applications. Powders having the phases of pure FeB with small amount of elemental Zn were obtained with a uniform morphology and an average particle size of 68 nm. The NPs were then coated with oleic acid (OA) and surface modified with sodium tricitrate, to increase their stability and biocompatibility, and well-dispersed NPs were obtained with sizes below 30 nm. TEM investigations revealed the presence of hybrid clusters with nanoparticle - OA structures, indicating that FeB nanoparticles were stabilized by being embedded in OA clusters, forming both agglomerated sub-micron and free nano-sized structures. Obtained NPs showed ferromagnetic property, with a saturation magnetization of 25.9 emu/g and a low coercivity of 90 Oe. As a result of testing different types of healthy and cancer cell lines with NPs, Zn-doped-FeB@OA NPs exhibited a high biocompatibility. Results suggested that highly biocompatible and magnetic OA-coated Zn-doped FeB particles can be potential candidates for biomedical applications such as medical imaging or drug delivery systems.Article Citation - WoS: 6Citation - Scopus: 7A Comprehensive Study on Doxorubicin-Loaded Aspartic Acid-Coated Magnetic Fe<sub>3</Sub>o<sub>4< Nanoparticles: Synthesis, Characterization and in Vitro Anticancer Investigations(Elsevier, 2024) Jafari, Nahideh; Mohammadpourfard, Mousa; Hamishehkar, HamedMagnetic Fe3O4 nanoparticles (MNPs) hold significant potential across various scientific fields due to their notable properties. For biomedical applications, MNPs must be biocompatible, stable, and possess high magnetic potential. Aspartic acid (ASP) as a coating agent not only provides biocompatibility, stability, and high magnetic potential but also offers the potential for absorbing various drugs for targeted delivery due to its carboxyl and amino functional groups. So, in this study, we synthesized ASP-coated MNPs (ASP-MNPs) through a one-step co-precipitation method and loaded doxorubicin (DOX) onto these nanoparticles to create DOX-ASP-MNPs for targeted drug delivery. Characterization of the nanoparticle confirmed the crystal structure, spherical morphology, and improved size distribution of ASP-MNPs (8.53 +/- 2.56 nm) compared to uncoated MNPs (7.05 +/- 1.89 nm), as analyzed by XRD, FESEM, and TEM. FT-IR and zeta potential assessments (ZP = -6.3 mV for MNPs, ZP = -31.1 mV for ASP-MNPs) verified successful ASP binding, DOX loading, and nanoparticle stability. VSM analysis indicated a slight decrease in saturation magnetism after coating (51.1 emu/g) compared to MNPs (57.4 emu/g). In vitro release studies demonstrated a higher release rate (83 %) of DOX-ASP-MNPs at pH 5.2, indicating their suitability for cancerous cells. Cytotoxicity assays on A-549 cancer cell lines showed a dose-dependent response. DAPI staining revealed that free DOX caused more DNA damage. Cellular uptake studies indicated a time-dependent uptake of DOX-ASP-MNPs, higher at 3 h compared to 1 h, though lower than free DOX uptake due to different uptake pathways. Apoptosis assays over 72 h showed similar apoptotic rates for DOX-ASP-MNPs and free DOX. These findings suggest that ASP-MNPs possess enhanced physicochemical properties and effective drug delivery capabilities, making them a promising candidate for different biomedical applications, particularly targeted cancer therapy.Article Citation - WoS: 13Citation - Scopus: 12Dunaliella Salina Microalgae Aqueous Extract-Based Magnetic Nanoparticles (fe3o4-Nps): Green Synthesis, Characterization and in Vitro Anticancer Investigations(Elsevier B.V., 2024) Jafari,N.; Hamishehkar,H.; Mohammadpourfard,M.In the current study, a facile, rapid, and eco-friendly method was provided for green synthesizing of magnetic Fe3O4-NPs with enhanced stability and biocompatibility using different weight concentrations (1 %, 3 %, and 5 %) of aqueous extract of Dunaliella salina (D. salina) microalgae. The properties of the green-synthesized magnetic nanoparticles (GMNPs-ex1%, GMNPs-ex3%, and GMNPs-ex5%) were compared with chemically-synthesized ones (CMNPs) via XRD, TEM, FESEM, VSM, DLS, ZP, FTIR, hemolysis, cell viability, DAPI staining, and apoptosis analyses. TEM imaging revealed mean sizes of 11.21 ± 2.63 nm and 14.08 ± 3.24 nm for GMNPs-ex5% and CMNPs, respectively, with better dispersity for GMNPs-ex5%, as confirmed with their polydispersity index (PDI = 0.24 for GMNPs-ex5% and 0.58 for CMNPs). These sizes were consistent with the crystallite size of pure magnetite phase nanoparticles obtained from XRD. FESEM images confirmed spherical shape for the majority of nanoparticles. FTIR spectra confirmed the involvement of functional groups from the extract in GMNPs, contributing to their stability (ZP of GMNPs-ex5% = −34 mV). The saturation magnetization decreased with increasing the extract ratio (from 62.41 to 8.94 emu/g), attributed to the non-magnetic nature of the extract coating. GMNPs-ex5% exhibited a negligible hemolysis rate (< 2 %) compared to CMNPs. Furthermore, IC50 values of GMNPs and extract against HFF-2 and A549 cells were higher than those of CMNPs, indicating the biocompatibility of green-synthesized nanoparticles. In the DAPI staining method, GMNPs-ex5%, similar to the extract, caused less DNA damage to HFF-2 cells. Additionally, the apoptosis assay using annexin V/PI staining kit indicated that green-synthesized nanoparticles induced lower apoptosis in normal cells. Overall, this study highlights the potential of green-synthesized Fe3O4-NPs for various biomedical applications, showcasing their enhanced properties and biocompatibility compared to conventionally synthesized counterparts. © 2024 Elsevier B.V.