Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Design Strategies to Optimize Polymeric Vectors for mRNA Delivery(Taylor and Francis Ltd., 2025) Bulmuş Zareie, Volga; Savaş, M.; Alakbarov, A.; Bulmus, V.; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologymRNA holds great promise for preventing and treating a variety of diseases, from infectious diseases to diverse cancers, owing to its transient expression, lack of genomic integration, and scalable production. Among non-viral vectors, polymeric carriers are attractive due to their synthetic versatility and stability, which allow for precise tuning for efficient mRNA delivery. Their scalability further supports the applicability of polymeric vectors. Studies have addressed the room for improvement in polymeric systems for mRNA delivery and have adapted varying approaches depending on the type of polymeric structure, including but not limited to PEGylation, hydrophobic modification, and incorporation of responsive or targeting moieties. This review summarizes advances in polymeric vectors for mRNA delivery and highlights how distinct structural modifications influence toxicity, mRNA transfection efficiency, biodistribution, intracellular trafficking and immune activation, providing a framework for the rational design of next-generation polymeric vectors that can fully realize the clinical potential of mRNA therapeutics. © 2025 Taylor & Francis Group, LLC.Conference Object Citation - Scopus: 2Co2 Capture by Pei-Impregnated Alumina Sorbents(ISRES Publishing, 2023) Turgut, Furkan; Kostik, Simge; Çağlar, Başar; Çağlar, Başar; 03.06. Department of Energy Systems Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyDirect air capture (DAC) or direct CO2 extraction from ambient air is a promising approach to reduce greenhouse gas emissions caused by both distributed (location independent) and point sources (location specific). Solid sorbents have been considered as more effective for DAC compared to the liquid counterpart since they have a faster kinetic and avoid volatile and heat losses due to the absence of evaporation of liquids. In this study, the alumina-supported polyethyleneimine (PEI) material was chosen as solid sorbents and their CO2 capture performance for different PEI loadings (20, 35, 50 wt%), flow rate (15, 30, 45 L/h) and adsorption temperatures (30, 40, 50, 60 °C) was investigated. Sorbents were prepared by using wetness impregnation method and their physical and chemical properties were characterized by several techniques such as N2 adsorption-desorption (surface area, pore size and volume), Scanning Electron Microscopy-SEM (surface morphology, surface chemical composition). The CO2 capture performance of sorbents were analyzed under different CO2 concentrations and the cyclic (adsorption-desorption) behavior of the sorbents were tested. The results show that alumina-supported PEI adsorbents are promising materials for CO2 capture with high CO2 adsorption capacity and stability. © 2023 Published by ISRES.Article Citation - Scopus: 1A Diaminoethane Motif Bearing Low Molecular Weight Polymer as a New Nucleic Acid Delivery Agent(Elsevier, 2021) Bulmuş Zareie, Volga; Ünal, Yağmur Ceren; Meşe Özçivici, Gülistan; Bulmuş, Volga; 01. Izmir Institute of Technology; 04.03. Department of Molecular Biology and Genetics; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 04. Faculty of ScienceAmong polymer-based gene delivery systems, poly(ethylene imine) (PEI) stands out as an effective polycation. However, the toxic effects of PEI especially at higher molecular weights limit its usage. Although the effects of PEI's architecture and molecular weight on gene delivery is controversial in literature, low molecular weight PEI appears to be efficient at transfection while having lower toxicity. Herein, as an alternative to low molecular weight, linear PEI, a methacrylate polymer bearing diamimoethane motifs, poly(2-((2-aminoethyl)amino)ethyl methacrylate) (P(AEAEMA)), was evaluated in vitro as a new nucleic acid delivery agent. P(AEAEMA) (8 kDa) showed low toxicity on Skov-3-luc and NIH/3T3 cell lines at polymer concentrations where PEI (8 kDa) was highly toxic. P(AEAEMA) could efficiently form complexes with siRNA at an N/P ratio of 2 as shown by gel electrophoresis. The diameter of P(AEAEMA)-siRNA complexes was found to be significantly lower than PEIsiRNA complexes almost at all tested N/P ratios. P(AEAEMA) could improve the stability of siRNA in serum containing media by protecting the siRNA against serum nucleases. siRNA and pDNA transfection efficiency of P (AEAEMA) on luciferase expressing Skov-3-luc cell line and HEK 293T cell line, respectively was found to be comparable to well-known nucleic acid carrier, PEI. The transfection efficiency of both P(AEAEMA) and PEI was found to be cell-type-dependent. None of the polymers were able to transfect MDA-MB-231 cells with siRNA or pDNA.