Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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Access type: Metadata onlyAuthor: search.filters.author.Karakuzu, Betul

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  • Article
    Creatinine-On Colorimetric Elisa-Based Serum Creatinine Detection in a Microfluidic Device
    (Royal Soc Chemistry, 2025) Karakuzu, Betul; Tekin, H. Cumhur
    Chronic kidney diseases (CKDs), which often end in kidney failure for many people around the world, have an important place in public health given that they also trigger other diseases. Therefore, the development of fast and cost-effective diagnostic technologies enables effective monitoring of patients and early diagnosis. Here, using the Enzyme-Linked Immunosorbent Assay (ELISA) principle, serum creatinine concentrations were determined using the developed lab-on-a-chip (LOC) platform. In this system, which was termed "creatinine-on-a-chip", colorimetric ELISA protocol was applied to determine creatinine levels in a microfluidic chip functionalized with creatinine-specific antibodies. Creatinine detection was performed by quantifying the absorbance difference between the detection and reference channels, normalized to the reference signal within the microfluidic chip. The detection signal intensity varied depending on the region selected along the microfluidic channel. The adsorption of the capture antibody used for surface functionalization, which was particularly more pronounced near the inlet region, played a critical role in the detection signal. These findings suggest that random selection of the detection area can lead to significant signal variability, and that careful selection of a well-characterized region is essential for improving detection performance. With this developed system, creatinine was detected with high sensitivity in the linear range of 1-20 mu g mL-1, both spiked in phosphate buffered saline (PBS) and fetal bovine serum (FBS). Using the creatinine-on-a-chip, serum creatinine analysis can be performed rapidly (similar to 15 min) in a cost-effective manner ($1.05 per test).
  • Conference Object
    Citation - Scopus: 1
    Colorimetric Detection of Creatinine on an Electromechanical Mixing Platform
    (IEEE, 2021) Tarim, E. Alperay; Oksuz, Cemre; Karakuzu, Betul; Tekin, H. Cumhur
    We present an electromechanical mixing platform integrated with a smartphone for colorimetric detection of creatinine using an enzymatic reaction between horseradish peroxidase (HRP) and 3,3',5,5'-Tetramethylbenzidine (TMB). On the developed platform, the color resulting from the reaction between HRP and TMB in a reservoir of a poly(methyl methacrylate) (PMMA) chip is measured using a smartphone camera. With mixing, diluted creatine solutions can be detected in 1 min that can reduce long waiting times of creatinine detection due to enzymatic reactions. Furthermore, smartphone-based colorimetric detection reduces the cost of analysis by eliminating costly equipment for spectrometric measurements without affecting the sensitivity of analysis. We, therefore, think that the presented platform integrated with a smartphone could be used for automatic measurement of creatinine level in the sample by allowing low-cost and rapid analysis, and this would be particularly beneficial for monitoring of chronic kidney disease (CKD) at the point-of-care setting.