Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
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Article Citation - WoS: 3Citation - Scopus: 4Caffeic Acid Phenethyl Ester (cape) Chitosan Capped Zno Nanoparticles: Preparation, Characterization, and Its Potential for the Treatment of Prostate Cancer(Elsevier B.V., 2024) İnce,İ.; Yıldırım,Y.; Göker,E.; Güler,G.; Saltan,F.; Acar,R.; Medine,E.İ.The synthesis of zinc oxide nanoparticles/chitosan (ZnONPs/CS) formulation loaded with Caffeic acid phenethyl ester (CAPE) was performed to evaluate its prostate cancer treatment efficiency within the scope of this research. It has been hypothesized that a dual active materials delivery system containing ZnO and CAPE loaded Chitosan (CS) nanoparticles has better bioavailability compared to single one against to cancer cells. ZnONPs were synthesized between 45 and 60 nm particle sizes and then they were capped with CS biodegradable polymer prior to load with CAPE bioactive molecule. ZnONPs/CS-CAPE system was characterized by using Fourier Transform Infrared (FTIR) for structural elucidation, Scanning Electron Microscope (SEM) for particle size determination, High Performance Liquid Chromatography (HPLC) system for determination of CAPE amount. 131I CAPE and 131I ZnONPs/CS-CAPE labeled by the Iodogen method with 131I were used in-vitro cell culture experiments. Cell viabilities (%) of CAPE and ZnONPs/CS-CAPE were examined using Cell Counting Kit-8 assay on PC-3 (human adenocarcinoma prostate), LnCaP (human carcinoma prostate), and RWPE-1 (human normal prostate). IC50 values of ZnONPs /CS -CAPE on all cells were found 2-fold lower than neat CAPE. Based on the FTIR data, the most significant spectral changes (lipid, protein, nucleic acids, glycogen) were monitored for the PC-3 and LnCaP cancer cells incubated with ZnONPs/CS-CAPE samples while being exposed to neat CAPE molecules caused small cellular changes when compared to RWPE-1 healthy cell lines. © 2024Article Citation - WoS: 3Citation - Scopus: 3Development of Xylan-Coated Acid-Resistant Micellar Drug Carriers for Colon-Targeted Oral Delivery(Taylor & Francis As, 2024) Zeybek, Nuket; Polat, Hurriyet; Gulec, Sukru; Buyukkileci, Ali OguzOral delivery of hydrophobic drugs from the stomach through the colon has some requirements: (1) an acid-resistant carrier (2) a colon-specific drug release mechanism; and (3) an enhanced bioavailability. In this study, curcumin-loaded polymeric micelles with a xylan-based composite coating were designed and developed. For this purpose, a new synthesis method was used to precipitate xylan by concurrent chitosan polymerization at different xylan/chitosan ratios using a negatively charged crosslinking agent, TPP. The study was to provide the stability of the coated micellar structures in the stomach (low pH conditions) and their degradation in the colon (a natural environment of bacteria) to release the drug. It was observed that the coating successfully prevented early drug release up to 85%, depending on the fraction of xylan in the coating. The nanocarriers that first passed through the stomach conditions were incubated with a xylanolytic colonic bacterium (Bacteroides ovatus) to determine the bacterium-related release mechanism, which was around 27%. This shows the colon-specific release expectation of coated nanocarriers in the colon environment, with an additional benefit due to the degradation of xylan and an improvement in the colon environment by prebiotic activity.Article Citation - WoS: 10Citation - Scopus: 10An Efficient Method of Improving Essential Oil Retention and Sustained Release of Chitosan Films: Ultrasound-Assisted Preparation of Chitosan Composites With Surface Active Chickpea Proteins(Elsevier, 2024) Barış Kavur, Pelin; Yemenicioğlu, AhmetThis work aimed at preparing chitosan (CHI) composites with surface active chickpea protein (CP) showing better eugenol (EUG) retention and sustained release capacity than pristine CHI films. For this purpose, ionic complexation of CHI with CP (CHI:CP ratio = 2:1, w/w) in the presence of EUG at pH 5.0 was achieved using mechanical homogenization alone (HM) or in combination with ultrasonic homogenization (HM-HUS). The HM-HUS treatment provided better solubility of CP (4.4-fold), increased emulsified EUG in film-forming solutions, and denser films than HM treatment. The composite films obtained using HM-HUS (FLMCHI-CP-EUG/HM-HUS) retained 1.2–1.4-fold higher EUG after drying, and showed almost 2-fold slower EUG release in air at room temperature than composite films prepared by HM, and control CHI films prepared by HM (FLMCHI-EUG/HM) or HM-HUS (FLMCHI-EUG/HM-HUS). The FLMCHI-CP-EUG/HM-HUS films also showed better moisture barrier and mechanical properties than other films. The developed films were proved in a challenging coating application with onions. Escherichia coli and Listeria innocua counts of inoculated and FLMCHI-CP-EUG/HM-HUS (average coating thickness = 4.5 ± 1.3 μm) coated onions were significantly lower than those of uncoated (2.8 and 3.8 log) and FLMCHI/HM-HUS (1.4 and 1.3 log) coated onions after 5-days at room temperature. FLMCHI-CP-EUG/HM-HUS coating also reduced percentage of sprouted onions from 30 to 10% during storage. EUG odor of coated onions could not have been detected by 80% of panelists after 4 weeks. Compositing with CP boosts the performance of essential oil loaded CHI films by enabling use of film matrix as an encapsulant. © 2024 Elsevier LtdArticle Citation - WoS: 15Citation - Scopus: 15Granulation of Hydrometallurgically Synthesized Spinel Lithium Manganese Oxide Using Cross-Linked Chitosan for Lithium Adsorption From Water(Elsevier B.V., 2024) Recepoğlu,Y.K.; Arabacı,B.; Kahvecioğlu,A.; Yüksel,A.A drastic increase in demand for electric vehicles and energy storage systems increases lithium (Li) need as a critical metal for the 21st century. Lithium manganese oxides stand out among inorganic adsorbents because of their high capacity, chemical stability, selectivity, and affordability for lithium recovery from aqueous media. This study investigates using hydrometallurgically synthesized lithium manganese oxide (Li1.6Mn1.6O4) in granular form coated with cross-linked chitosan for lithium recovery from water. Characterization methods such as SEM, FTIR, XRD, and BET reveal the successful synthesis of the composite adsorbent. Granular cross-linked chitosan-coated and delithiated lithium manganese oxide (CTS/HMO) adsorbent demonstrated optimal removal efficiency of 86 % at pH 12 with 4 g/L of adsorbent dosage. The Langmuir isotherm at 25 °C, which showed monolayer adsorption with a maximum capacity of 4.94 mg/g, a better fit for the adsorption behavior of CTS/HMO. Adsorption was endothermic and thermodynamically spontaneous. Lithium adsorption followed the pseudo-first-order kinetic model. © 2024Review Citation - WoS: 13Citation - Scopus: 13Oxygen Delivery Biomaterials in Wound Healing Applications(WILEY-V C H VERLAG GMBH, 2023) Bayraktar, Sema; Üstün, Cansu; Kehr, Nermin SedaOxygen (O2) delivery biomaterials have attracted great interest in the treatment of chronic wounds due to their potential applications in local and continuous O2 generation and delivery, improving cell viability until vascularization occurs, promoting structural growth of new blood vessels, simulating collagen synthesis, killing bacteria and reducing hypoxia-induced tissue damage. Therefore, different types of O2 delivery biomaterials including thin polymer films, fibers, hydrogels, or nanocomposite hydrogels have been developed to provide controlled, sufficient and long-lasting O2 to prevent hypoxia and maintain cell viability until the engineered tissue is vascularized by the host system. These biomaterials are made by various approaches, such as encapsulating O2 releasing molecules into hydrogels, polymer microspheres and 3D printed hydrogel scaffolds and adsorbing O2 carrying reagents into polymer films of fibers. In this article, different O2 generating sources such as solid inorganic peroxides, liquid peroxides, and photosynthetic microalgae, and O2 carrying perfluorocarbons and hemoglobin are presented and the applications of O2 delivery biomaterials in promoting wound healing are discussed. Furthermore, challenges encountered and future perspectives are highlighted. Oxygen delivery (O2) biomaterials have attracted great interest in the treatment of chronic wounds due to their ability to continuously deliver oxygen and support cell viability. Therefore, various O2 generating sources such as solid inorganic peroxides, liquid peroxides and photosynthetic microalgae, and O2-carrying perfluorocarbons and hemoglobin are incorporated into different biomaterial networks for wound healing applications.imageArticle Citation - WoS: 6Citation - Scopus: 8Development of Pro-Angiogenic Wound Dressings From 2-Deoxy (2ddr)-Loaded Decellularized Plant Leaves(SPRINGER, 2023) Dikici, Serkan; Çavdaroğlu, ÇağrıTraditional wound dressings are essential for the treatment of acute and superficial wounds. However, complex wounds require the use of bioactive dressings that promote healing alongside providing a safe barrier for the coverage of the wound site. The addition of growth factors is usually the primary choice to fabricate functionalized wound dressing. However, it is also the main reason for the increase in the cost of a wound dressing and may be associated with several drawbacks, such as the need for a precise drug delivery system to be able to be administered at a narrow effective dose range. 2-deoxy-D-ribose (2dDR) is a cost-effective and promising pro-angiogenic agent that indirectly stimulates vascular endothelial growth factor production to stimulate angiogenesis, and consecutively accelerate wound healing. In this study, we aimed to fabricate a novel wound dressing from 2dDR-loaded decellularized spinach leaves and evaluated its bioactivity on human endothelial cells in vitro. Our results demonstrated that a biocompatible wound dressing biomaterial could successfully be fabricated via the decellularization of spinach leaves using chemical decellularization. The success of decellularization was confirmed quantitatively and qualitatively via determination of the DNA content and Fourier transform infrared spectroscopy, respectively. 2dDR was then easily incorporated into the dressings via physical absorption and released from them in 5 days. The release of 2dDR-releasing decellularized spinach leaves was observed to increase the viability and metabolic activity of human endothelial cells in vitro over 7 days. In conclusion, we demonstrated the fabrication of a novel functionalized biomaterial combining decellularized plant tissues with a promising pro-angiogenic agent, and 2dDR-loaded decellularized spinach leaves appear to have great potential to be used as a bioactive wound dressing to promote angiogenesis and, consecutively, wound healing.Article Citation - WoS: 9Citation - Scopus: 8Development of a New Electrochemical Sensor Based on Molecularly Imprinted Biopolymer for Determination of 4,4'-methylene Diphenyl Diamine(MDPI, 2023) Ghaani, Masoud; Büyüktaş, Duygu; Carullo, Daniele; Farris, StefanoA new molecularly imprinted electrochemical sensor was proposed to determine 4,4' methylene diphenyl diamine (MDA) using molecularly imprinted polymer-multiwalled carbon nanotubes modified glassy carbon electrode (MIP/MWCNTs/GCE). GCE was coated by MWCNTs (MWCNTs/GCE) because of their antifouling qualities and in order to improve the sensor sensitivity. To make the whole sensor, a polymeric film made up of chitosan nanoparticles was electrodeposited by the cyclic voltammetry method on the surface of MWCNTs/GCE in the presence of MDA as a template. Different parameters such as scan cycles, elution time, incubation time, molar ratio of template molecules to functional monomers, and pH were optimized to increase the performance of the MIP sensor. With a detection limit of 15 nM, a linear response to MDA was seen in the concentration range of 0.5-100 mu M. The imprinting factor (IF) of the proposed sensor was also calculated at around 3.66, demonstrating the extremely high recognition performance of a MIP/MWCNT-modified electrode. Moreover, the sensor exhibited good reproducibility and selectivity. Finally, the proposed sensor was efficiently used to determine MDA in real samples with satisfactory recoveries ranging from 94.10% to 106.76%.Article Citation - WoS: 6Citation - Scopus: 7Designing Robust Xylan/Chitosan Composite Shells Around Drug-Loaded Msns: Stability in Upper Git and Degradation in the Colon Microbiota(Elsevier, 2023) Zeybek, Nüket; Büyükkileci, Ali Oğuz; Güleç, Şükrü; Polat, Mehmet; Polat, Hürriyetong residence times, near-neutral pH values, and release triggered by the enzymatic action of the resident microbiota offer unique opportunities for improved drug delivery in the colon. The fact that a delivery agent must also pass through the complete GI tract without degradation presents a challenge due to widely changing pH conditions. In this study, a promising colon-targeted drug delivery system was composed of a xylan/chitosan composite shell formed on curcumin-loaded mesoporous silica nanoparticles (MSNs). A novel synthesis approach was employed to facilitate precipitation of negatively charged xylan on negatively charged MSNs by concurrent chitosan polymerization. Curcumin-loaded xylan/chitosan-coated MSNs (C-MSNs) were determined to contain nearly 42% xylan by the inclusion of chitosan in a one-to-one ratio with xylan. The xylan/chitosan composite shell demonstrated excellent stability in the acidic upper GI tract. The hydrolysis of glycosidic bonds by resident microbiota was the triggering mechanism for xylan degradation and curcumin release in the colon. The presence of xylan has the further benefit of increasing the number of beneficial bacteria and improving short-chain fatty acid production for improved colon health.Article Citation - WoS: 5Citation - Scopus: 5The Designing of a Gel Formulation With Chitosan Polymer Using Liposomes as Nanocarriers of Amphotericin B for a Non-Invasive Treatment Model of Cutaneous Leishmaniasis(Springer, 2022) Gürbüz, Nergiz; Çetin Uyanıkgil, Emel Öykü; Özbel, Yusuf; Töz, SerayPurpose Leishmaniasis is a disease caused by different Leishmania spp., which are transmitted to humans by a bite of infected female sand flies. Cutaneous leishmaniasis (CL, oriental sore), visceral leishmaniasis (VL), and mucocutaneous leishmaniasis (MCL) are three main clinical forms, however, only CL and VL are seen in Turkey. Cutaneous leishmaniasis is characterized by skin lesion(s) and is one of the most important vector-borne diseases in Turkey with over 2000 cases reported annually in 40 out of 81 provinces. The treatment is usually made invasively and painfully by intralesional injection of pentavalent antimony compounds. Non-invasive and innovative treatment methods are needed as aimed in this study. Methods In the present study, one of the classical antileishmanial drugs, amphotericin B (AmB), encapsulated in liposomes was evaluated using non-invasive design based on chitosan, which is a nontoxic, biocompatible and biodegradable polymer. To avoid the invasive effect of conventional intralesional needle application, the drug was encapsulated in liposomes and incorporated into a chitosan gel for applying topically on the skin lesion. The efficacy of encapsulation of amphotericin B into liposomes and the drug release from liposomes were studied. The chitosan gel was evaluated for viscosity, flowability, appearance and pH. The efficacy of the drug embedded into chitosan gel, liposomal AmB alone and chitosan gel alone in four different concentrations was also tested using Leishmania spp. promastigotes in vitro. Results The findings have shown that AmB was encapsulated into the liposomes with high efficiency (86.6%) and long-term physical and chemical stability. Therefore, designed liposomal formulation was suitable for sustained release. The appearance of the drug-embedded chitosan gel was transparent and appropriate. Chitosan gels showed non- Newtonian behavior and plastic flow. The liposomal AmB also showed higher efficacy with no parasites in all concentrations while drug embedded into chitosan gel and chitosan gel alone were effective in two higher concentrations. The lower efficacy of the drug-embedded chitosan gel in 24 h in in-vitro study was probably due to slow release of the drug. Conclusion The gel design created in this study will provide ease of use for the lesions of CL patients that do not have a specific number, size, and shape. Follow-up studies by the ex-vivo macrophage infection model with Leishmania intracellular amastigote forms and Leishmania-infected animal models are needed to understand the present design's efficacy better.Article Citation - WoS: 22Citation - Scopus: 23Bioactive Snail Mucus-Slime Extract Loaded Chitosan Scaffolds for Hard Tissue Regeneration: the Effect of Mucoadhesive and Antibacterial Extracts on Physical Characteristics and Bioactivity of Chitosan Matrix(IOP Publishing, 2021) Perpelek, Merve; Tamburacı, Sedef; Aydemir, Selma; Tıhmınlıoğlu, Funda; Baykara, Başak; Karakaşlı, Ahmet; Havıtçıoğlu, HasanBiobased extracts comprise various bioactive components and they are widely used in tissue engineering applications to increase bioactivity as well as physical characteristics of biomaterials. Among animal sources, garden snail Helix aspersa has come into prominence with its antibacterial and regenerative extracts and show potential in tissue regeneration. Thus, in this study, bioactive H. aspersa extracts (slime, mucus) were loaded in chitosan (CHI) matrix to fabricate porous scaffolds for hard tissue regeneration. Physical, chemical properties, antimicrobial activity was determined as well as in vitro bioactivity for bone and cartilage regeneration. Mucus and slime incorporation enhanced mechanical properties and biodegradation rate of CHI matrix. Scanning electron microscopy images showed that the average pore size of the scaffolds decreased with higher extract content. Mucus and slime extracts showed antimicrobial effect on two bacterial strains. In vitro cytotoxicity, osteogenic and chondrogenic activity of the scaffolds were evaluated with Saos-2 and SW1353 cell lines in terms of Alkaline phosphatase activity, biomineralization, GAG, COMP and hydroxyproline content. Cell viability results showed that extracts had a proliferative effect on Saos-2 and SW1353 cells when compared to the control group. Mucus and slime extract loading increased osteogenic and chondrogenic activity. Thus, the bioactive extract loaded CHI scaffolds showed potential for bone and cartilage regeneration with enhanced physical properties and in vitro bioactivity.