Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 1Citation - Scopus: 1Peptide-Functionalized Hydrocolloid Bioink for 3D Bioprinting in Dental Tissue Engineering(Elsevier, 2025) Guner, Elif; Yildirim-Semerci, Ozum; Altan, Zeynep; Arslan-Yildiz, AhuDeveloping biomimetic peptide-based biomaterials has utmost importance to enhance mineralization offering an innovative approach for dental tissue regeneration. This study comprises development and characterization of a novel peptide-based hybrid bioink for dental tissue engineering applications by integrating P11-4 peptide and Gelatin (Gel) into glucuronoxylan-based quince seed hydrocolloid (QSH). Combining polysaccharide and peptide-based hydrogels enhanced cell adhesion and mineralization. Morphological analysis showed that P11-4 increased porosity, while rheological tests confirmed that QSH/Gel/P11-4 bioink has tunable viscosity, which is suitable for 3D bioprinting. Optimized bioprinting parameters were determined to be 25G nozzle diameter, 10 mm/s speed of movement, 0.1 mm layer height, and pressure values of 9.0 and 7.0 psi for QSH/Gel and QSH/ Gel/P11-4, respectively. Moreover, the addition of P11-4 significantly increased protein adsorption without affecting swelling capacity. 3D cell culture studies were conducted using SaOS-2 (human osteosarcoma) cells, then biocompatibility, high cell viability, favored adhesion, and proliferation were confirmed by Live/Dead and MTT assays. Alizarin Red Staining (ARS) and EDX analysis verified that P11-4 promoted mineral deposition by increasing Calcium (Ca2+) accumulation in QSH/Gel/P11-4 scaffolds, suggesting that developed bioink can mimic native ECM microenvironment for dental tissue. Overall, the developed hybrid bioink shows superior printability and bioactivity, which makes it a promising material for 3D bioprinting applications in dental tissue engineering.Article Citation - WoS: 1From Chemistry to Clinic: Polysaccharide-Bioceramic Composites for Tissue Engineering Applications(Mary Ann Liebert, Inc, 2025) Yakubogullari, Nilgun; Yilmaz-Dagdeviren, Hilal Deniz; Arslan-Yildiz, AhuComposite scaffolds combining polysaccharides and bioceramics represent next-generation scaffolds extensively investigated in tissue engineering (TE) and biomedical applications. Polysaccharides such as chitosan, hyaluronic acid, and pectin mimic the extracellular matrix components with their tunable physicochemical properties, enabling a favorable microenvironment for cell adhesion, proliferation, and cell-matrix interactions. On the other hand, bioceramics, including calcium phosphate, hydroxyapatite, and bioactive glasses, enhance the mechanical properties of the material and offer structural integrity and osteoconductive properties. While they have generally been preferred to be used in bone TE and dental applications, various studies have also demonstrated their potential in cartilage regeneration, wound healing, and broader biomedical applications. Recent advancements in material design and scaffold fabrication techniques, particularly 3D printing and electrospinning, have provided precise engineering of materials and fabrication of scaffolds for desirable mechanical properties and biological performance. These innovations foster the development of patient-specific scaffolds, thereby paving the way for applications in personalized medicine. This review critically summarizes alternative polysaccharides, bioceramics, and composite materials used in TE and biomedical applications. It also highlights advanced fabrication strategies and finally explores the translational potential of these biocomposites. By integrating emerging technologies, this review aims to provide alternative and sustainable materials for the development of next-generation scaffolds that meet clinical needs.Impact Statement This study introduces polysaccharide-bioceramic composites with enhanced mechanical and biological properties for tissue engineering. Beyond bone and dental repair, their applications increasingly extend to wound healing, cartilage, cardiac, and muscle regeneration with drug delivery, angiogenesis, and neurogenesis. By mimicking the native extracellular matrix, these composites support cell growth and tissue regeneration, offering a versatile platform for advanced regenerative therapies.Article Citation - WoS: 5Citation - Scopus: 6Hydrocolloids for Tissue Engineering and 3d Bioprinting(World Scientific Publ Co Pte Ltd, 2024) Yildirim-Semerci, Ozum; Onbas, Rabia; Bilginer-Kartal, Rumeysa; Arslan-Yildiz, AhuHydrocolloids, derived from plants, marine, and microbial sources, have become research favorites due to their unique properties. This article provides an overview of the extraction methods, from chemical to enzymatic, to obtain hydrocolloids. Distinctive properties of hydrocolloids, such as high swelling capacity, tunable features, and rapid gelation ability, have gained significant attention recently and have started to be used in tissue engineering and 3D bioprinting. Hydrocolloids will play substantial roles in advancing biomedical products and contributing to improving human health.Article Citation - WoS: 2Citation - Scopus: 3Magnetic Levitational Assembly of Differentiated Sh-Sy5y Cells for Aβ-Induced 3d Alzheimer's Disease Modeling and Curcumin Screening(Wiley-v C H verlag Gmbh, 2025) Bilginer-Kartal, Rumeysa; Arslan-Yildiz, AhuAlzheimer's disease is one of the prevalent neurodegenerative diseases and is characterized by amyloid beta aggregate (A beta) accumulation. This study reports an A beta 1-42 induced 3D Alzheimer's disease modeling utilizing differentiated SH-SY5Y spheroids, which is carried out by Magnetic levitation approach, and the neuroprotective effect of Curcumin is further investigated on this model. For this purpose, SH-SY5Y spheroids are differentiated using Retinoic acid-Brain-derived neurotrophic factor sequentially during 3D cell culture. Differentiated spheroids maintained high viability and exhibited significant neuronal characteristics, as evidenced by increasing beta-III tubulin and NeuN expressions. 3D Alzheimer's disease model formation and neurotoxicity of A beta 1-42 aggregates are investigated on un-/differentiated spheroids, resulting in 65% and 51% cell viability, respectively. Characterization of the 3D Alzheimer's disease model is done by immunostaining of Choline acetyltransferase to investigate cholinergic neuron activity loss, showing a 2.2 decrease in fluorescence intensity. Further, Curcumin treatment on the 3D Alzheimer's disease model resulted in augmenting cell viability, confirming neuroprotective effect of Curcumin on A beta 1-42 induced Alzheimer's disease model. This study highlighted the magnetic levitation-based fabrication of A beta 1-42-induced 3D Alzheimer's disease model successfully, offering a promising experimental platform for other neurodegenerative disease research and potential clinical applications.Article Citation - WoS: 2Citation - Scopus: 2Exploring the Use of Water-Extracted Flaxseed Hydrocolloids in Three-Dimensional Cell Culture(Mary Ann Liebert, inc, 2024) Yildirim-Semerci, Ozum; Bilginer-Kartal, Rumeysa; Arslan-Yildiz, AhuPlant-derived hydrocolloids offer promising prospects in biomedical applications. Among these, Flaxseed hydrocolloid (FSH) can form a soft, elastic, and biocompatible hydrocolloid with tunable viscosity and superior swelling capacity, making it an attractive scaffold. This study introduces a green extraction method for FSH, employing a single-step aqueous extraction process and fabrication of FSH scaffold. Despite growing interest, the pristine form of FSH has not been investigated for sustainable long-term three-dimensional (3D) cell culture. Here, FSH scaffolds were thoroughly characterized for their morphological, chemical, mechanical, and biological properties. 3D cell culture experiments were conducted using NIH-3T3 mouse fibroblast cells, and cell viability was assessed using live/dead and Alamar Blue assays. High cell viability was sustained for long term compared with 2D cell culture. Cell adhesion and 3D cellular morphology on FSH scaffold for 30 days were monitored by scanning electron microscopy analysis. Also, collagen type-I and F-actin expressions were analyzed by immunostaining after 30 days of culture, resulting in 5- and 4-fold increments of fluorescence intensity, respectively. Results indicate sustained cell viability in the long term and favorable cell-material interaction, demonstrating the potential of FSH as a scaffold. This study emphasizes the importance of the green extraction approach, improving the biocompatibility and functionality of FSH tissue engineering applications. Impact Statement Flaxseed hydrocolloid (FSH) is a promising scaffold for biomedical applications due to its biocompatibility and tunable properties. This study introduces a green extraction method for FSH and evaluates its use in 3D cell culture with NIH-3T3 mouse fibroblast cells. The findings indicate high cell viability and enhanced cell-material interactions over 30 days, highlighting the potential of FSH for tissue engineering.Article Citation - WoS: 2Citation - Scopus: 3Development of Mg-Alginate Based Self Disassociative Bio-Ink for Magnetic Bio-Patterning of 3d Tumor Models(Wiley-v C H verlag Gmbh, 2024) Coban, Basak; Baskurt, Mehmet; Sahin, Hasan; Arslan-Yildiz, AhuAlginate forms a hydrogel via physical cross-linking with divalent cations. In literature, Ca2+ is mostly utilized due to strong interactions but additional procedures are required to disassociate Ca-alginate hydrogels. On the other hand, Mg-alginate hydrogels disassociate spontaneously, which might benefit certain applications. This study introduces Mg-alginate as the main component of a bio-ink for the first time to obtain 3D tumor models by magnetic bio-patterning technique. The bio-ink contains magnetic nanoparticles (MNPs) for magnetic manipulation, Mg-alginate hydrogel as a sacrificial material, and cells. The applicability of the methodology is tested for the formation of 3D tumor models using HeLa, SaOS-2, and SH-SY5Y cells. Long-term cultures are examined by Live/dead and MTT analysis and revealed high cell viability. Subsequently, Collagen and F-actin expressions are observed successfully in 3D tumor models. Finally, the anti-cancer drug Doxorubicin (DOX) effect is investigated on 3D tumor models, and IC50 values is calculated to assess the drug response. As a result, significantly higher drug resistance is observed for bio-patterned 3D tumor models up to tenfold compared to 2D control. Overall, Mg-alginate hydrogel is successfully used to form bio-patterned 3D tumor models, and the applicability of the model is shown effectively, especially as a drug screening platform.Article Citation - WoS: 2Citation - Scopus: 2Origami-Inspired Microfluidic Paper-Based Analytical Device (μpad) for Microorganism Detection(Springernature, 2024) Sozmen, A. Baran; Bayraktar, A. Ezgi; Arslan-Yildiz, AhuPathogenic microorganisms impose great risk especially in resource-limited settings due to inaccessibility of diagnostic tools and monitoring devices. This is mainly caused by current methods often being economically demanding and complex in practice; while these methods are sensitive and accurate, they rarely follow Point-of-care (POC) approaches, which is essential for rapid detection and intervention. Incorporating origami into paper-based analytical devices (mu PAD) presents an innovative alternative, offering affordability, portability, and ease of disposal. Herein, a colorimetric origami mu PAD that is suitable for use in POC applications was developed. The mu PAD was fabricated via laser ablation utilizing PVDF and cellulose membranes. In order to develop the biosensor platform, fabrication parameters were optimized and hydrophilicity of PVDF membranes was improved using various solvents. The PVDF membranes were characterized through light microscopy imaging, protein adsorption assay and contact angle measurements. Then, optimization of the assay parameters was carried out in order to improve sensitivity and resolution of the mu PAD, utilizing Box-Behnken experimental design. The responses generated by the origami mu PAD in form of visible color development were then analyzed using image processing. After optimization is concluded, E. coli detection was carried out as a model system. Resulting calculations showed a limit of detection (LoD) of 2 CFU/mL and a dynamic working range up to 106 CFU/mL for E. coli. Overall, developed origami mu PAD promises an economic advantage compared to conventional methods, and provides rapid and sensitive results without the requirement of expertise or complex equipment.Article Citation - WoS: 17Citation - Scopus: 18Utilizing Magnetic Levitation To Detect Lung Cancer-Associated Exosomes(Amer Chemical Soc, 2024) Sozmen, Alper Baran; Arslan-Yildiz, AhuExtracellular vesicles, especially exosomes, have attracted attention in the last few decades as novel cancer biomarkers. Exosomal membrane proteins provide easy-to-reach targets and can be utilized as information sources of their parent cells. In this study, a MagLev-based, highly sensitive, and versatile biosensor platform for detecting minor differences in the density of suspended objects is proposed for exosome detection. The developed platform utilizes antibody-functionalized microspheres to capture exosomal membrane proteins (ExoMPs) EpCAM, CD81, and CD151 as markers for cancerous exosomes, exosomes, and non-small cell lung cancer (NSCLC)-derived exosomes, respectively. Initially, the platform was utilized for protein detection and quantification by targeting solubilized ExoMPs, and a dynamic range of 1-100 nM, with LoD values of 1.324, 0.638, and 0.722 nM for EpCAM, CD81, and CD151, were observed, respectively. Then, the sensor platform was tested using exosome isolates derived from NSCLC cell line A549 and MRC5 healthy lung fibroblast cell line. It was shown that the sensor platform is able to detect and differentiate exosomal biomarkers derived from cancerous and non-cancerous cell lines. Overall, this innovative, simple, and rapid method shows great potential for the early diagnosis of lung cancer through exosomal biomarker detection.