Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Book Part Citation - Scopus: 9Differential Expression of Toxoplasma Gondii Micrornas in Murine and Human Hosts(Springer, 2016) Allmer, Jens; Saçar Demirci, Müşerref Duygu; Bağcı, CanerMicroRNAs are short RNA sequences involved in post-transcriptional gene regulation. MicroRNAs are known for a wide variety of species ranging from bacteria to plants. It has become clear that some cross-kingdom regulation is possible especially between viruses and their hosts. We hypothesized that intracellular parasites, like Toxoplasma gondii, similar to viruses would be able to modulate their host’s gene expression. We were able to show that T. gondii produces many putative pre-miRNAs which are actually transcribed. Furthermore, some of these expressed pre-miRNAs have a striking resemblance to host mature miRNAs. Previous studies indicated that T. gondii infection coincides with increased abundance of some miRNAs. Here we were able to show that many of these miRNAs have close relatives in T. gondii which may not be distinguishable using PCR. Taken together, the similarity to host miRNAs, their confirmed expression, and their upregulation during infection, it suggests that T. gondii actively transfers miRNAs to regulate its host. We conclude, that this type of cross-kingdom regulation may be possible, but that targeted analysis is necessary to consolidate our computational findings. © Springer International Publishing Switzerland 2016. All rights are reserved.Conference Object Citation - Scopus: 1Removing Contamination From Genomic Sequences Based on Vector Reference Libraries(Institute of Electrical and Electronics Engineers Inc., 2012) Bağcı, Caner; Allmer, JensDNA is often sequenced after being cloned into a vector since this provides the possibility for using standard primers and removes the need to develop custom primers. In this way a certain amount of vector is sequenced along with the sequence of interest. Unfortunately, occasionally these contaminating vector sequences find their way into public databases as part of submitted sequences. It has been pointed out that SeqClean, a program used to remove vector contamination from sequences, does not take into account that vectors are circular structures. A workaround has been presented before, but we were able to simplify the process and, additionally, we provide an implementation. We further applied our method to a test set of EST sequences and also analyzed the amount of contamination found in the EST sequences available on NCBI. © 2012 IEEE.