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Citation - WoS: 11
Citation - Scopus: 22
Genome-Wide Snp Discovery and Genetic Linkage Map Construction in Sunflower (helianthus Annuus L.) Using a Genotyping by Sequencing (gbs) Approach
(Springer Verlag, 2016) Çelik, İbrahim; Bodur, Sabahattin; Frary, Anne; Doğanlar, Sami
Recently developed plant genomics approaches (LD mapping and genome-wide selection) require many molecular markers distributed throughout the plant genome. As a result, the availability of an increasing number of markers is essential for maintaining highly efficient and accurate plant breeding programs. In this study, we identified SNP loci in sunflower using a genotyping by sequencing (GBS) approach in an intraspecific F2 mapping population. A total of 271,445,770 reads were generated by the Genome Analyzer II next-generation sequencing platform and 29.2 % of the reads were aligned to unique locations in the genome. A total of 46,278 SNP loci were identified and 7646 SNP loci were validated in an F2 population. In addition, a SNP-based linkage map was constructed. This is the first report of SNP discovery in sunflower by GBS. The SNP markers and SNP-based linkage map will be valuable molecular genetics tools for sunflower breeding.

Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection