Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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Author: search.filters.author.Bozkurt, BerkayScopus Q: search.filters.scopusQuartile.Q4

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  • Article
    The Effectiveness of Genetic Markers and the Role of Environmental Factors in Hip Dysplasia and Osteochondritis Dissecans of the Shoulder in German Shepherd, Labrador Retriever, and German Wirehaired Pointer (Deutsch Drahthaar) Dogs
    (Elsevier, 2025) Ardicli, Sena; Yigitgor, Pelin; Ozen, Dogukan; Babayev, Huseyn; Bozkurt, Berkay; Senturk, Nursen; Intas, Deniz Seyrek
    Canine Hip Dysplasia (CHD) is the most frequently diagnosed orthopedic condition in dogs. Similar to CHD, osteochondritis dissecans (OCD) of the shoulder is a developmental disorder in dogs that significantly impacts animal welfare. As polygenic genetic disorders, they exhibit a complex mode of inheritance. Although there are numerous clinical studies, there is insufficient information about the genetic basis of these disorders. Therefore, this study aimed to assess the relationship of the prognostic genetic test markers with CHD and OCD in German Shepherd, Labrador Retriever, and German Wirehaired Pointer dogs. We evaluated the efficiency of five SNP markers from the prognostic genetic test for CHD (the Dysgen test) based on available GWAS data in German Shepherd, Labrador Retriever, and German Wirehaired Pointer dogs. Radiographs were captured and assessed according to the official FCI scale for hip dysplasia. In German Wirehaired Pointers, shoulder X-ray evaluations were also performed. We used custom FRET-based primer probes in Real-time PCR and Sanger sequencing for genotyping and tested the evaluation using multiple logistic regression procedures. German shepherds emerged as the most vulnerable to CHD (P < 0.001). In the final logistic model, females are expected to have a 3.54 times higher likelihood of experiencing CHD compared to males (P < 0.05). SNP BICF2G630558239 demonstrated a notable association with CHD, indicating that the GG genotype poses a risk. This SNP is situated in the intronic region of the KIF26B gene, a member of the kinesin superfamily implicated in evolutionarily conserved roles in embryogenesis. We did not observe any association between shoulder OCD-related arthrosis and the SNPs studied. These results may contribute to understanding CHD by identifying genotypes associated with epidemiological risk, prompting the need to conduct more thorough investigations.
  • Article
    Expression of Steroidogenic Enzymes in Placentome of Ewes With Pregnancy Toxemia After Two Parturition Induction Methods
    (Hellenic Veterinary Medical Society, 2023) Risvanlı, A.; Özalp, G. R.; Ortaç, C. T.; Bozkurt, Berkay; Aktar, A.; Yavuz, A.; Korlu, Y.; Şeker, İ.
    The regulation pattern of important enzymes in placental steroidogenesis and prostaglandin production in ewes with pregnancy toxemia is reviewed. The alterations of gene expressions after the administration of aglepristone (AG) and dexamethasone (DEX) are also discussed. Four healthy (CG) and 22 ewes with experimental pregnancy toxemia were included in the study. Ewes with pregnancy toxemia of group AG (n=9) and group DEX (n=9) were injected twice with 10 mg/kg of aglepristone and once with 5 ml dexamethasone respectively to induce parturition on 141 & PLUSMN;1,3 day of gestation; whereas healthy control [Group CG (n=4)] and pregnancy toxemia [Group PT (n=4)] group received no treatment for parturition induction. Placentomes were immediately collected right after the expulsion of the last lamb. mRNA extraction from total placentome capsule, cotyledon and caruncle was carried out and Real-Time PCR was performed. Serum samples were collected from ewes and cortisol, PGFM, PGE2, estrone sulfate and progesterone concentrations were measured after treatments until parturition. The lowest mRNA expressions of steroidogenic enzymes were detected in group PT. Interestingly expression pattern of steroidogenic enzymes in group AG was similar to group PT. No difference was found in mRNA expressions of 3 & beta;HSD and CYP19 among groups. Between groups, AG-DEX the mRNA expressions in the caruncle of PTGS2/COX2 and PGFS were statistically different respectively (P<0.005). A significant difference could be observed in EP3 expression in the caruncle of DEX and AG compared to CG (P<0.05); however PTGES, EP1, EP2, and EP4 expressions were not statistically different among groups (P>0,05). Estrone sulfate, PGE,2 and PGFM concentrations were statistically different, however, no difference was observed in cortisol levels between groups. The present study suggests that the endocrinologic pathway controlling parturition is different in ewes with pregnancy toxemia. Dexamethasone administration endocrinologically mimicked normal partu-rition, but the genes regulating uterine contractions were similarly expressed, as in group PT. Probably expressions of EP1 and tissue-specific counter-expressions of cervical EP genes could refer to the pathogenesis of insufficient cervical dilatation, observed in pregnancy toxemia and dexamethasone applications.