Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

Browse

Filters

2021 - 20262

Settings

Author: search.filters.author.Demirci, FatihScopus Q: search.filters.scopusQuartile.Q1

Search Results

Now showing 1 - 2 of 2
  • Article
    Lipoxygenase Inhibitory Activity Evaluation of Achillea Biebersteinii Afan. by Activity-Guided Fractionation
    (Elsevier Ireland Ltd, 2026) Subasi, Bilgen; Gunbatan, Tugba; Gurbuz, Ihan; Dilmac, Elif; Bedir, Erdal; Demirci, Fatih
    Ethnopharmacological relevance: Achillea biebersteinii Afan. is traditionally utilized as folk medicine for a broad range of therapeutic applications, especially for promoting the maturation of abscesses, wound healing; against inflammation, and rheumatism in T & uuml;rkiye. Aim of the study: The anti-inflammatory potential of A. biebersteinii was evaluated through activity-guided fractionation (AGF) targeting lipoxygenase (15-LOX) inhibition. Materials and methods: Different chromatographic techniques were used for the AGF of the ethyl acetate extract of A. biebersteinii aerial parts. The in vitro 15-LOX inhibitory activity evaluation was performed to address the antiinflammatory activity. The structures of purified compounds from the fractions were confirmed by LC-HRMS, 1H NMR, and 13C NMR analyses, respectively. Results: The fractionation and isolation process culminated in the identification of three key flavonoids namely; patulitrin, axillarin, quercetagetin-7-O-beta-glucopyranoside, and 4,5-dicaffeoylquinic acid, which showed statistically remarkable 15-LOX inhibitory activity with inhibition rates of 82.27%, 96.81 %, 84.65% and 77.47 %, respectively. Two flavonoids were isolated by using the AGF method, where quinic acid was spotted to have significant 15-LOX inhibitory activity. Conclusion: These findings support the future therapeutic potential of A. biebersteinii as a natural antiinflammatory source.
  • Article
    Citation - WoS: 12
    Citation - Scopus: 12
    Isolation of Rosmarinic Acid and Methyl Rosmarinate as Lipoxygenase Inhibitors From Salvia Palaestina Benth. by Activity-Guided Fractionation
    (Elsevier, 2021) İçen, Mehmet Sina; Gürbüz, İlhan; Bedir, Erdal; Günbatan, Tuğba; Demirci, Fatih
    Salvia palaestina aqueous and methanol extracts were prepared from the aerial parts, which were evaluated for the in vitro anti-inflammatory properties using the lipoxygenase (LO) enzyme inhibition assay. While the aqueous extract showed no activity at test concentrations, a significant (p < 0.001) lipoxygenase inhibition was detected for the methanol extract with 29% inhibition. Activity guided fractionation was carried out on the methanol extract via liquid-liquid partitioning using n-hexane, dichloromethane, ethyl acetate, and n-butanol. The ethyl acetate fraction showed statistically the best inhibition among the sub-fractions with 70% inhibition (p < 0.0001). The compounds responsible for the activity were purified, and their structures were established as rosmarinic acid, and methyl rosmarinate by spectroscopic methods. IC50 values of rosmarinic acid, and methyl rosmarinate were calculated as 0.21 and 0.02 ?M, respectively. In conclusion, the in vitro anti-inflammatory potential of S. palaestina was associated to rosmarinic acid, and methyl rosmarinate, for the first time to the best of our knowledge. © 2021 SAAB