Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 6Citation - Scopus: 7Mineral Nutrient Acquisition by Cotton Cultivars Grown Under Salt Stress(Taylor and Francis Ltd., 2017) Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yalçın, İbrahim ErtuğrulPhysiological responses were investigated in two cotton cultivars grown at various concentrations of sodium chloride (NaCl) in order to determine the degree of the tolerance of the cultivars to salt stress and understand the physiological responses with respect to utilization of mineral nutrients. After germination of the seeds of cotton cultivars, they were transferred into standard pots with 210 g sterilized compost and watered with 30 ml Hoagland’s solution containing different concentrations (0, 50, 100, 200, and 400 mM) of NaCl at two-day intervals for 3 months. Growth parameters were measured and the mineral nutrient analyses were done using inductively coupled plasma optical emission spectrometry (ICP-OES, Thermo Fisher Scientific, Waltman, MA). It was observed that plant growth and mineral nutritional status of both cultivars were altered extensively in those grown with NaCl. Excess NaCl reduces the concentrations of certain mineral nutrients and increases that of others, the patterns depending on the mineral nutrient and the plant part and varieties being compared to the control.Article Citation - WoS: 6Citation - Scopus: 8Mineral Element Uptake Status of Endemic Isoetes Anatolica Prada & Rolleri Populations From Bolu-Turkey(Pakistan Botanical Society, 2013) Özyiğit, İbrahim İlker; Doğan, İlhan; Eskin, Bülent; Keskin, Mustafa; Demir, Göksel; Yalçın, İbrahim ErtuğrulIsoetes genus is commonly known as the "quillworts" and considered to be "fern allies". There are about 200-250 species, with a cosmopolitan distribution but often scarce to rare. Isoetes genus members often grow in extremely sensitive aquatic environments such as temporary ponds, streams and lakes. They are therefore good indicators of environmental quality. Isoetes anatolica Prada & Rolleri is an endemic plant grows on calcareous sediment/soil on the edges of seasonal ponds located in a mountainous area near the southern coast of the Black Sea at 1400 m above sea level at Bolu, Turkey. In this study, mineral element uptake statuses of I. anatolica populations were studied on the background of plant-sediment/soil-water interactions. The study materials were collected from the place where this narrow endemic species only lives in the world (Abant Region, Bolu/Turkey) by using standard methods and plant and sediment/soil mineral element measurements (Al, B, Ca, Cu, Fe, K, Mg, Mn, Na, Ni and Zn) were done. ICP-OES was employed for the measurements during the study. Interrelations between mineral element contents in the sediment/soil, water and plant were discussed. The data revealed that I. anatolica is capable of accumulating considerable amounts of certain mineral elements (B, Ca, Mn and Na).Article Citation - WoS: 2Citation - Scopus: 9Germination Physiology and Autecology of Centaurea Kilaea Boiss. From Turkey(Penerbit Universiti Kebangsaan Malaysia, 2013) Eskin, B.; Özyiğit, İbrahim İlker; Doğan, İlhan; Altay, V.; Demir, G.; Serin, M.In this study germination requirements, plant-soil interactions and population biology of Centaurea kilaea was studied. The plant and soil samples were collected from Sofular Village (Sile District) and shore of Çatalca District (Istanbul) in Turkey by using standard methods. Methods like Scheibler, Wetdigestion, Kjeldahl and Olsen were employed for measurement of soil texture, structure and other physical and chemical characteristics (pH, total protein and electrical conductivity) using spectrophotometer, flame photometer, calcimeter and ICP. The results showed that ranges of different elements in the soil were 0.007-0.2% for N, 0.0007-0.001% for P, 0.001-0.01% for K, 0.0001-0.0002 % for Na. N, P, K and Na values in the plants were 2.17, 0.005, 0.1 and 0.006%, respectively. The data revealed that germination success of the seeds was influenced by the environmental factors such as pH, germination season and temperature.Article Citation - WoS: 2Citation - Scopus: 2Distribution of Some Elements in Veronica Scutellata L. From Bolu, Turkey: Soil-Plant Interactions(Penerbit Universiti Kebangsaan Malaysia, 2013) Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, G.; Eskin, B.; Keskin, M.; Yalçın, İbrahim ErtuğrulVeronica scutellata L. occurs in moist and wet habitats, such as ponds, marshes and other wetlands. This study was conducted on this species to examine its mineral element uptake status in terms of interactions between soil and plant. Experimental materials were taken from the Southern coast of Black Sea at coordinates 40°36'N and 31°16'E at an altitude of 1400 m above sea level from Bolu - Turkey; using standard methods and plant (root, stem and leaf parts) and soil mineral element measurements (Al, B, Ca, Cu, Fe, K, Mg, Mn, Na, Ni and Zn) were done. During the study, ICP-OES was employed for the measurement of mineral elements. It was observed that considerable amounts of B, Ca, K, Mg, Mn, Na and Zn are accumulated by the plant.Article Citation - WoS: 11Citation - Scopus: 15Salinity Induced Changes in Cotton (gossypium Hirsutum L.)(Pakistan Botanical Society, 2012) Doğan, İlhan; Kekeç, Güzin; Özyiğit, İbrahim İlker; Sakçalı, Mehmet SerdalCotton (Gossypium hirsutum L.) is susceptible to abiotic stresses. High salinity is a common abiotic stress condition that adversely affects plant growth. Altered ion and water homeostasis changes due to NaCI stress, lead to molecular damage, growth arrest and even death. As a consequence of salt stress effects, secondary stresses such as oxidative damage by reactive oxygen species may occur. Reactive oxygen species can alter cellular metabolism through oxidative damage of lipids, proteins and nucleic acids causing lipid peroxidation, protein denaturing and DNA mutation. In recent years, several selective and sensitive assays have been developed to evaluate the effects of environmental stress on vegetal organisms. RAPD is one of them and developed for DNA analysis. In this study, cotton seedlings were used as bioindicator of salinity stress in the range of 50-400 mM. Effects of salinity stress were determined by comparing RAPD profiles of normal and treated cotton seedlings include variations in band intensities as well as gains or losses of band numbers. The DNA polymorphisms detected by RAPD analysis could be used as an investigation tool and useful biomarker assay for observing environmental stresses such as high salinity on vegetal organisms.Article Citation - WoS: 18Citation - Scopus: 16Determination of Some Heavy Metals and Mineral Nutrients of Bay Tree (laurus Nobilis L.) in Bartin City, Turkey(Pakistan Botanical Society, 2012) Yaşar, Ülkühan; Özyiğit, İbrahim İlker; Yalçın, İbrahim Ertuğrul; Doğan, İlhan; Demir, GökselConcentrations of Al, Cd, Cu, Ni, and Pb in Laurus nobilis L. were examined for assessment of the impact of heavy metal exposure during winter periods, since these metals have the highest toxic potential. In this study, leaf (washed and unwashed), bark and branch samples of L. nobilis and soil samples were collected from 13 different localities, belonged to three stations. In conjunction with analyzing impact of the heavy metal exposure on the city using L. nobilis as a biomonitoring tool, the uptake and composition of mineral nutrients of L. nobilis were also investigated for determining the effects of heavy metals on mineral nutrition metabolism of the plant. The heavy metal and mineral nutrient concentrations of the collected samples were measured by using ICP-OES. The obtained data was analyzed with SPSS statistics program. As a result of measurements, the lowest and highest heavy metal accumulations and the amount of mineral nutrients measured in plants were as follows; Al (14.69-122.44 mg/kg d. wt), Cd (0.23-0.89 mg/kg d. wt), Cu (1.64-14.25 mg/kg d. wt.), Ni (0.001-0.45 mg/kg d. wt.), Pb (2.06-5.28 mg/kg d. wt.) and B (1.04- 6.67 mg/kg d. wt.), Ca (1195.34-4919.03 mg/kg d. wt.), Fe (17.13-203.25 mg/kg d. wt.), K (538.99-3778.37 mg/kg d. wt.), Mg(48.1-268.5 mg/kg d. wt.), Na (24.91-77.43 mg/kg d. wt.) and Zn (4.75-15.74 mg/kg d. wt.). According to the experimental data, the volume of the air pollution was analyzed and found significant in the city. Also, it was noticed that the metabolism of mineral nutrients of L. nobilis was altered by heavy metals. Finally, it was proved that L. nobilis is a suitable organism to be used as a biomonitoring tool for conducting research on heavy metal pollution.Article Citation - WoS: 12Citation - Scopus: 15Mineral Element Distribution of Cotton (gossypium Hirsutum L.) Seedlings Under Different Salinity Levels(Pakistan Botanical Society, 2012) Doğan, İlhan; Özyiğit, İbrahim İlker; Demir, GökselCotton (Gossypium hirsutum L.) is the world's leading natural fiber and second largest oilseed crop. In addition to textile manufacturing, cotton and cotton-by products are the sources of wealth of consumer based products, livestock feed, fertilizer, foodstuff and paper. High concentrations of NaCl in soils account for large decreases in the yield of a wide variety of crops all over the world. The present study was conducted to evaluate NaCl stress on mineral nutrient composition of cotton due to its economic importance. Cotton seeds were germinated in Magenta vessels containing Murshige and Skoog (MS) media for 15 days and then transferred in sterile jars containing MS exposed to different levels of NaCl (50, 100, 200 and 400 mM) treatments for 1 month. Uptake of some mineral nutrients (B, Ca, Fe, K, Mg, Mn, Na and Zn) by the plants was examined in roots and leaves by using an Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES). The data proved that plant growth and uptake and accumulation of microelements are altered extensively in cotton grown with NaCl. Excess NaCl reduces the uptake pattern of certain elements and increases that of others, the patterns depending on the element and the plant part being compared to the control.Article Citation - WoS: 36Citation - Scopus: 46Expression of Vitreoscilla Hemoglobin in Gordonia Amarae Enhances Biosurfactant Production(Springer Verlag, 2006) Doğan, İlhan; Pagilla, Krishna R.; Webster, Dale A.; Stark, Benjamin C.The gene (vgb) encoding Vitreoscilla (bacterial) hemoglobin (VHb) was electroporated into Gordonia amarae, where it was stably maintained, and expressed at about 4 nmol VHb g -1 of cells. The maximum cell mass (OD 600) of vgb-bearing G. amarae was greater than that of untransformed G. amarae for a variety of media and aeration conditions (2.8-fold under normal aeration and 3.4-fold under limited aeration in rich medium, and 3.5-fold under normal aeration and 3.2-fold under limited aeration in mineral salts medium). The maximum level of trehalose lipid from cultures grown in rich medium plus hexadecane was also increased for the recombinant strain, by 4.0-fold in broth and 1.8-fold in cells under normal aeration and 2.1-fold in broth and 1.4-fold in cells under limited aeration. Maximum overall biosurfactant production was also increased in the engineered strain, by 1.4-fold and 2.4-fold for limited and normal aeration, respectively. The engineered strain may be an improved source for producing purified biosurfactant or an aid to microorganisms bioremediating sparingly soluble contaminants in situ.