Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Review Citation - WoS: 13Citation - Scopus: 13Oxygen Delivery Biomaterials in Wound Healing Applications(WILEY-V C H VERLAG GMBH, 2023) Bayraktar, Sema; Üstün, Cansu; Kehr, Nermin SedaOxygen (O2) delivery biomaterials have attracted great interest in the treatment of chronic wounds due to their potential applications in local and continuous O2 generation and delivery, improving cell viability until vascularization occurs, promoting structural growth of new blood vessels, simulating collagen synthesis, killing bacteria and reducing hypoxia-induced tissue damage. Therefore, different types of O2 delivery biomaterials including thin polymer films, fibers, hydrogels, or nanocomposite hydrogels have been developed to provide controlled, sufficient and long-lasting O2 to prevent hypoxia and maintain cell viability until the engineered tissue is vascularized by the host system. These biomaterials are made by various approaches, such as encapsulating O2 releasing molecules into hydrogels, polymer microspheres and 3D printed hydrogel scaffolds and adsorbing O2 carrying reagents into polymer films of fibers. In this article, different O2 generating sources such as solid inorganic peroxides, liquid peroxides, and photosynthetic microalgae, and O2 carrying perfluorocarbons and hemoglobin are presented and the applications of O2 delivery biomaterials in promoting wound healing are discussed. Furthermore, challenges encountered and future perspectives are highlighted. Oxygen delivery (O2) biomaterials have attracted great interest in the treatment of chronic wounds due to their ability to continuously deliver oxygen and support cell viability. Therefore, various O2 generating sources such as solid inorganic peroxides, liquid peroxides and photosynthetic microalgae, and O2-carrying perfluorocarbons and hemoglobin are incorporated into different biomaterial networks for wound healing applications.imageArticle Citation - WoS: 6Citation - Scopus: 8Development of Pro-Angiogenic Wound Dressings From 2-Deoxy (2ddr)-Loaded Decellularized Plant Leaves(SPRINGER, 2023) Dikici, Serkan; Çavdaroğlu, ÇağrıTraditional wound dressings are essential for the treatment of acute and superficial wounds. However, complex wounds require the use of bioactive dressings that promote healing alongside providing a safe barrier for the coverage of the wound site. The addition of growth factors is usually the primary choice to fabricate functionalized wound dressing. However, it is also the main reason for the increase in the cost of a wound dressing and may be associated with several drawbacks, such as the need for a precise drug delivery system to be able to be administered at a narrow effective dose range. 2-deoxy-D-ribose (2dDR) is a cost-effective and promising pro-angiogenic agent that indirectly stimulates vascular endothelial growth factor production to stimulate angiogenesis, and consecutively accelerate wound healing. In this study, we aimed to fabricate a novel wound dressing from 2dDR-loaded decellularized spinach leaves and evaluated its bioactivity on human endothelial cells in vitro. Our results demonstrated that a biocompatible wound dressing biomaterial could successfully be fabricated via the decellularization of spinach leaves using chemical decellularization. The success of decellularization was confirmed quantitatively and qualitatively via determination of the DNA content and Fourier transform infrared spectroscopy, respectively. 2dDR was then easily incorporated into the dressings via physical absorption and released from them in 5 days. The release of 2dDR-releasing decellularized spinach leaves was observed to increase the viability and metabolic activity of human endothelial cells in vitro over 7 days. In conclusion, we demonstrated the fabrication of a novel functionalized biomaterial combining decellularized plant tissues with a promising pro-angiogenic agent, and 2dDR-loaded decellularized spinach leaves appear to have great potential to be used as a bioactive wound dressing to promote angiogenesis and, consecutively, wound healing.Article Citation - WoS: 5Citation - Scopus: 9Using Loofah Reinforced Chitosan-Collagen Hydrogel Based Scaffolds In-Vitro and In-Vivo; Healing in Cartilage Tissue Defects(Elsevier B.V., 2023) Baysan, G.; Gunes, O.C.; Turemis, C.; Akokay, Yilmaz, P.; Husemoglu, R.B.; Kara, Ozenler, A.; Perpelek, M.The herein article aims to report a new scaffold design as a loofah-reinforced chitosan-collagen hydrogel composite scaffold with three different cross-linker concentrations (0.1, 0.3, and 0.5 wt. /v%). From the analyses, the scaffold crosslinked with 0.5% genipin; collagen-chitosan hydrogel scaffold reinforced with loofah (L-CCol5) was found to be suitable for further in vitro and in vivo studies due to its interconnected porous structure, water content (∼ 97%) and tan delta (0.221 at 1 Hz) values comparable to that of cartilage tissue. In vitro analyses depicted that the L-CCol5 scaffold supported rabbit mesenchymal stem cells (rMSCs) adhesion and proliferation with its non-cytotoxic feature. Moreover, in vivo cartilage healing studies were performed using New Zealand male rabbits in three groups: empty control, cell-free scaffold, and rMSCs-laden scaffold. The elastic moduli of these three groups were 0.69, 0.90, and 1.18 MPa, respectively. Besides, microcomputer tomography (MicroCT) scannings supported the in vivo biomechanical analyses as cell-laden scaffolds showed better osteochondral healing. It can be concluded that the L-CCol5 scaffold could be a promising construct in osteochondral tissue engineering applications. The findings revealed that osteochondral remodeling precedes articular cartilage, providing insight into tailored therapeutic approaches, disease progress, and treatment consequences. © 2023 Acta Materialia Inc.