Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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  • Article
    Novel Neural Style Transfer Based Data Synthesis Method for Phase-Contrast Wound Healing Assay Images
    (Elsevier Ltd, 2024) Erdem,Y.S.; Iheme,L.O.; Uçar,M.; Özuysal,Ö.Y.; Balıkçı,M.; Morani,K.; Ünay,D.
    Recent advancements in the field of image synthesis have led to the development of Neural Style Transfer (NST) and Generative Adversarial Networks (GANs) which have proven to be powerful tools for data augmentation and realistic data generation. While GANs have been widely used for both data augmentation and generation, NST has not been employed for data generation tasks. Nonetheless, the simpler structure of NST compared to GANs makes it a promising alternative. In this research, we introduce an NST-based method for data generation, which to the best of our knowledge, is the first of its kind. By taking advantage of simplified architecture of NST models attributed to the utilization of a real image as the style input, our method enhances performance in data generation tasks under limited resource conditions. Additionally by utilizing patch-based training and high-resolution inference process high quality images are synthesized with limited resources. Furthermore multi-model and noised input is utilized for increased diversity with the novel NST-based data generation approach. Our proposed method utilizes binary segmentation maps as the condition input, representing the cell and wound regions. We evaluate the performance of our proposed NST-based method and compare it with a modified and fine-tuned conditional GAN (C-GAN) methods for the purpose of conditional generation of phase-contrast wound healing assay images. Through a series of quantitative and qualitative analyses, we demonstrate that our NST-based method outperforms the modified C-GAN while utilizing fewer resources. Additionally, we show that our NST-based method enhances segmentation performance when used as a data augmentation method. Our findings provide compelling evidence regarding the potential of NST for data generation tasks and its superiority over traditional GAN-based methods. The NST for data generation method was implemented in Python language and will be accessible at https://github.com/IDU-CVLab/NST_for_Gen under the MIT licence. © 2024 Elsevier Ltd
  • Article
    Citation - WoS: 8
    Citation - Scopus: 8
    Canonical Wnt and Tgf-β/Bmp Signaling Enhance Melanocyte Regeneration but Suppress Invasiveness, Migration, and Proliferation of Melanoma Cells
    (Frontiers Media S.A., 2023) Katkat, Esra; Demirci, Yeliz; Heger, Guillaume; Karagülle, Doğa; Papatheodorou, Irene; Brazma, Alvis; Özhan, Güneş
    Melanoma is the deadliest form of skin cancer and develops from the melanocytes that are responsible for the pigmentation of the skin. The skin is also a highly regenerative organ, harboring a pool of undifferentiated melanocyte stem cells that proliferate and differentiate into mature melanocytes during regenerative processes in the adult. Melanoma and melanocyte regeneration share remarkable cellular features, including activation of cell proliferation and migration. Yet, melanoma considerably differs from the regenerating melanocytes with respect to abnormal proliferation, invasive growth, and metastasis. Thus, it is likely that at the cellular level, melanoma resembles early stages of melanocyte regeneration with increased proliferation but separates from the later melanocyte regeneration stages due to reduced proliferation and enhanced differentiation. Here, by exploiting the zebrafish melanocytes that can efficiently regenerate and be induced to undergo malignant melanoma, we unravel the transcriptome profiles of the regenerating melanocytes during early and late regeneration and the melanocytic nevi and malignant melanoma. Our global comparison of the gene expression profiles of melanocyte regeneration and nevi/melanoma uncovers the opposite regulation of a substantial number of genes related to Wnt signaling and transforming growth factor beta (TGF-beta)/(bone morphogenetic protein) BMP signaling pathways between regeneration and cancer. Functional activation of canonical Wnt or TGF-beta/BMP pathways during melanocyte regeneration promoted melanocyte regeneration but potently suppressed the invasiveness, migration, and proliferation of human melanoma cells in vitro and in vivo. Therefore, the opposite regulation of signaling mechanisms between melanocyte regeneration and melanoma can be exploited to stop tumor growth and develop new anti-cancer therapies.