Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

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Scopus Q: search.filters.scopusQuartile.Q2Subject: search.filters.subject.Oxygen Delivery

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  • Article
    Citation - WoS: 1
    The Impact of Oxygen and Antimicrobial Tea Tree Oil Carrying Biomaterial on Cell Viability Under Hypoxic Conditions
    (Wiley, 2025) Demir, Yagmur Damla; Tepeli, Dilek; Guvensen, Mahmut Deniz; Soyer, Ferda; Akin, Ozlem; Kehr, Nermin Seda
    Traditional wound treatment involves protecting the wound with dressing and administering antibiotics to prevent tissue infection due to bacteria. However, these methods are inadequate due to the side effects of antibiotics on healthy cells and microbial resistance to antibiotics. Therefore, new strategies involving the application of natural resources such as essential oils as antimicrobial agents in combination with biomaterials as wound dressings have been tested in the treatment of wounds. Furthermore, oxygen (O2)-releasing biomaterials have attracted great interest due to the important role of O2 in wound healing processes. However, the co-application of O2 and essential oil as antimicrobial and cell-promoting agents has not been studied. In this context, we report a novel biomaterial capable of co-delivering O2 and natural antimicrobial tea tree oil (TTO) for 15 and 5 days, respectively. The biomaterial consists of an alginate scaffold (Alg-PMOF-O) containing O2-carrying nanomaterial, laponite and TTO. In vitro bacterial experiments have shown that O2 release from Alg-PMOF-O is an additional parameter acting as an antibacterial agent to inhibit bacterial growth but is not sufficient alone to inhibit bacteria. 5 mu L of TTO in Alg-PMOF-O is necessary to suppress both E. coli and S. aureus over a 1-day incubation period. The effect of TTO and O2 alone or in combination on cell viability is examined using WST-1 and PrestoBlue assays. According to the WST-1 and PrestoBlue tests, the combined application of TTO and O2 does not show any toxic effect on fibroblast cells under normoxic conditions during the 5-day incubation period. Under hypoxic conditions, the WST-1 test shows no toxic effect after only 1 day of incubation, while the PrestoBlue test shows no toxicity under hypoxia during both 1 and 5 days of incubation. On the other hand, the combined application of TTO and O2 indicates toxic effects on cancer Malme-3M cells during both normoxic and hypoxic conditions over 1 and 5 days of incubation. This effect is confirmed by both the WST-1 and PrestoBlue tests. The overall results demonstrate that Alg-PMOF-O exhibits antibacterial activity while having a lower toxic effect on fibroblasts under hypoxic conditions, and therefore has potential for use as wound dressing.
  • Article
    Citation - WoS: 2
    Citation - Scopus: 4
    Injectable Nanocomposite Hydrogels With Co-Delivery of Oxygen and Anticancer Drugs for Higher Cell Viability of Healthy Cells Than Cancer Cells Under Normoxic and Hypoxic Conditions
    (Iop Publishing Ltd, 2025) Kehr, Nermin Seda
    Injectable nanocomposite hydrogels (NC hydrogels) have the potential to be used for minimally invasive local drug delivery. In particular, pH-sensitive injectable NC hydrogels can be used in cancer treatment to deliver high doses of anticancer drugs to the target site in cancer tissue without damaging healthy tissue. Recent studies have shown that in addition to stimuli-responsive delivery of anticancer drugs to cancer cells, oxygen delivery to the hypoxic environment of cancer tissue can lead to advanced effects, as hypoxia and an acidic pH are common characteristics of cancer tissue. However, few studies have investigated the effects of simultaneous administration of oxygen (O2) and pH-dependent anticancer drugs via injectable NC hydrogels on the viability of healthy and cancer cells under normoxic and hypoxic conditions. In this context, we describe the synthesis of injectable NC hydrogels composed of pH-responsive nanomaterials carrying oxygen and anticancer drugs. Our system provides sustained O2 release and pH-responsive sustained release of anticancer drugs for 15 and 30 d, respectively. Moreover, O2 delivery and/or simultaneous delivery of O2 and anticancer drug resulted in higher cell survival of healthy fibroblast cells than malignant Colo-818 cells under hypoxic conditions (1% O2) after 7 d of incubation.