Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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Subject: search.filters.subject.BioimagingWoS Q: search.filters.wosQuartile.Q2

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  • Article
    Developing Gold Nanoparticles Decorated With Carbon-Dots for Multiplexed Cellular Imaging
    (IOP Publishing Ltd, 2025) Kavuranpala, Tugce; Saydullaeva, Iroda; Ozcelik, Serdar
    This study focuses on developing a novel hybrid nanomaterial composed of gold nanoparticle decorated with carbon dots, termed AuNP@C-dot, as a versatile platform for multiplexed imaging. Structural and spectral characterizations confirmed the successful conjugation of C-dots to AuNPs via covalent bonding, as evidenced by FTIR, X-ray photoelectron spectra, HRTEM analyses, and UV-Vis and fluorescence spectroscopies. The fluorescence intensities of C-dots are doubled through the conjugation to the AuNPs. The conjugation of fluorescent C-dots to plasmon-resonant AuNPs enables simultaneous multicellular imaging by taking advantage of the fluorescent signaling of C-dots and the scattering signaling of AuNPs. In vitro studies using human lung cell lines (A549 and BEAS-2B) confirmed the multiplexed imaging and revealed efficient cellular uptake and subcellular localization of AuNP@C-dots, including nuclear translocation, which is critical for radiotherapy and photodynamic therapy. Cell viability assessments utilizing a colorimetric assay for measuring cell metabolic activity and a colony formation assay demonstrated good biocompatibility of AuNP@C-dots at relevant concentrations. It can be envisioned that the AuNP@C-dot hybrid system may improve the detection and monitoring of cell health and disease due to its dual-modal imaging capability. Furthermore, they could be used for supervising controlled release of therapeutic agents, tailored for enhanced treatment efficacy. This study demonstrates the potential of C-dot-conjugated AuNPs as a multifunctional tool with inherent control mechanisms for the next-generation cellular analysis, drug administration, and diagnostic strategies.
  • Article
    Citation - WoS: 1
    Citation - Scopus: 1
    Gadolinium and Polythiophene Functionalized Polyurea Polymer Dots as Fluoro-Magnetic Nanoprobes
    (MDPI, 2022) Karabacak, Soner; Palaniappan, Alagappan; Tony, Tsang Siu Hon; Edwin, Teo Hang Tong; Gulyás, Balázs; Padmanabhan, Parasuraman; Yıldız, Ümit Hakan
    A rapid and one-pot synthesis of poly 3-thiopheneacetic acid (PTAA) functionalized pol-yurea polymer dots (Pdots) using polyethyleneimine and isophorone diisocyanate is reported. The one-pot mini-emulsion polymerization technique yielded Pdots with an average diameter of ~20 nm. The size, shape, and concentration of the surface functional groups could be controlled by al-tering the synthesis parameters such as ultrasonication time, concentration of the surfactant, and crosslinking agent, and the types of isocyanates utilized for the synthesis. Colloidal properties of Pdots were characterized using dynamic light scattering and zeta potential measurements. The spherical geometry of Pdots was confirmed by scanning electron microscopy. The Pdots were post-functionalized by 1,4,7,10 tetraazacyclododecane-1,4,7,10-tetraacetic acid for chelating gadolinium nanoparticles (Gd3+) that provide magnetic properties to the Pdots. Thus, the synthesized Pdots possess fluorescent and magnetic properties, imparted by PTAA and Gd3+, respectively. Fluorescence spectroscopy and microscopy revealed that the synthesized dual-functional Gd3+-Pdots ex-hibited detectable fluorescent signals even at lower concentrations. Magnetic levitation experiments indicated that the Gd3+-Pdots could be easily manipulated via an external magnetic field. These findings illustrate that the dua-functional Gd3+-Pdots could be potentially utilized as fluorescent reporters that can be magnetically manipulated for bioimaging applications.