Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
Browse
14 results
Search Results
Now showing 1 - 10 of 14
Article Citation - WoS: 10Citation - Scopus: 10An Efficient Method of Improving Essential Oil Retention and Sustained Release of Chitosan Films: Ultrasound-Assisted Preparation of Chitosan Composites With Surface Active Chickpea Proteins(Elsevier, 2024) Barış Kavur, Pelin; Yemenicioğlu, AhmetThis work aimed at preparing chitosan (CHI) composites with surface active chickpea protein (CP) showing better eugenol (EUG) retention and sustained release capacity than pristine CHI films. For this purpose, ionic complexation of CHI with CP (CHI:CP ratio = 2:1, w/w) in the presence of EUG at pH 5.0 was achieved using mechanical homogenization alone (HM) or in combination with ultrasonic homogenization (HM-HUS). The HM-HUS treatment provided better solubility of CP (4.4-fold), increased emulsified EUG in film-forming solutions, and denser films than HM treatment. The composite films obtained using HM-HUS (FLMCHI-CP-EUG/HM-HUS) retained 1.2–1.4-fold higher EUG after drying, and showed almost 2-fold slower EUG release in air at room temperature than composite films prepared by HM, and control CHI films prepared by HM (FLMCHI-EUG/HM) or HM-HUS (FLMCHI-EUG/HM-HUS). The FLMCHI-CP-EUG/HM-HUS films also showed better moisture barrier and mechanical properties than other films. The developed films were proved in a challenging coating application with onions. Escherichia coli and Listeria innocua counts of inoculated and FLMCHI-CP-EUG/HM-HUS (average coating thickness = 4.5 ± 1.3 μm) coated onions were significantly lower than those of uncoated (2.8 and 3.8 log) and FLMCHI/HM-HUS (1.4 and 1.3 log) coated onions after 5-days at room temperature. FLMCHI-CP-EUG/HM-HUS coating also reduced percentage of sprouted onions from 30 to 10% during storage. EUG odor of coated onions could not have been detected by 80% of panelists after 4 weeks. Compositing with CP boosts the performance of essential oil loaded CHI films by enabling use of film matrix as an encapsulant. © 2024 Elsevier LtdArticle Citation - WoS: 15Citation - Scopus: 15Granulation of Hydrometallurgically Synthesized Spinel Lithium Manganese Oxide Using Cross-Linked Chitosan for Lithium Adsorption From Water(Elsevier B.V., 2024) Recepoğlu,Y.K.; Arabacı,B.; Kahvecioğlu,A.; Yüksel,A.A drastic increase in demand for electric vehicles and energy storage systems increases lithium (Li) need as a critical metal for the 21st century. Lithium manganese oxides stand out among inorganic adsorbents because of their high capacity, chemical stability, selectivity, and affordability for lithium recovery from aqueous media. This study investigates using hydrometallurgically synthesized lithium manganese oxide (Li1.6Mn1.6O4) in granular form coated with cross-linked chitosan for lithium recovery from water. Characterization methods such as SEM, FTIR, XRD, and BET reveal the successful synthesis of the composite adsorbent. Granular cross-linked chitosan-coated and delithiated lithium manganese oxide (CTS/HMO) adsorbent demonstrated optimal removal efficiency of 86 % at pH 12 with 4 g/L of adsorbent dosage. The Langmuir isotherm at 25 °C, which showed monolayer adsorption with a maximum capacity of 4.94 mg/g, a better fit for the adsorption behavior of CTS/HMO. Adsorption was endothermic and thermodynamically spontaneous. Lithium adsorption followed the pseudo-first-order kinetic model. © 2024Article Citation - WoS: 6Citation - Scopus: 7Designing Robust Xylan/Chitosan Composite Shells Around Drug-Loaded Msns: Stability in Upper Git and Degradation in the Colon Microbiota(Elsevier, 2023) Zeybek, Nüket; Büyükkileci, Ali Oğuz; Güleç, Şükrü; Polat, Mehmet; Polat, Hürriyetong residence times, near-neutral pH values, and release triggered by the enzymatic action of the resident microbiota offer unique opportunities for improved drug delivery in the colon. The fact that a delivery agent must also pass through the complete GI tract without degradation presents a challenge due to widely changing pH conditions. In this study, a promising colon-targeted drug delivery system was composed of a xylan/chitosan composite shell formed on curcumin-loaded mesoporous silica nanoparticles (MSNs). A novel synthesis approach was employed to facilitate precipitation of negatively charged xylan on negatively charged MSNs by concurrent chitosan polymerization. Curcumin-loaded xylan/chitosan-coated MSNs (C-MSNs) were determined to contain nearly 42% xylan by the inclusion of chitosan in a one-to-one ratio with xylan. The xylan/chitosan composite shell demonstrated excellent stability in the acidic upper GI tract. The hydrolysis of glycosidic bonds by resident microbiota was the triggering mechanism for xylan degradation and curcumin release in the colon. The presence of xylan has the further benefit of increasing the number of beneficial bacteria and improving short-chain fatty acid production for improved colon health.Article Citation - WoS: 52Citation - Scopus: 54Development of Si Doped Nano Hydroxyapatite Reinforced Bilayer Chitosan Nanocomposite Barrier Membranes for Guided Bone Regeneration(Elsevier, 2021) Tamburacı, Sedef; Tıhmınlıoğlu, FundaGuided Bone Regeneration (GBR) is a widely used process for the treatment of periodontal defects to prevent the formation of surrounding soft tissue at the periodontal defect and to provide hard tissue regeneration. Recently GBR designs have focused on the development of resorbable natural polymer-based barrier membranes due to their biodegradability and excellent biocompatibility. The aim of this study is to fabricate a novel bilayer nanocomposite membrane with microporous sublayer composed of chitosan and Si doped nanohydroxyapatite particles (Si-nHap) and chitosan/PEO nanofiber upper layer. Bilayer membrane was designed to prevent epithelial and fibroblastic cell migration and growth impeding bone formation with its upper layer and to support osteogenic cell bioactivity at the defect site with its sublayer. Microporous and nanofiber layers were fabricated by using freeze-drying and electrospinning techniques respectively. The effect of Si-nHap content on the morphological, mechanical and physical properties of the composites were investigated using SEM, AFM, micro-Ct, compression test, water uptake capacity and enzymatic degradation study. Antimicrobial properties of nanocomposite membranes were investigated with tube dilution and disk diffusion methods. In vitro cytotoxicity of bilayer membranes was evaluated. Saos-2 and NIH/3T3 proliferation studies were carried out on each layer. In vitro bioactivity of Saos-2 and NIH/3T3 cells were evaluated with ALP activity and hydroxyproline content respectively. Results showed that Si-nHap incorporation enhanced the mechanical and physical properties as well as controlling biodegradability of the polymer matrix. Besides, Si-nHap loading induced the bioactivity of Saos-2 cells by enhancing cell attachment, spreading and biomineralization on the material surface. Thus, results supported that designed bilayer nanocomposite membranes can be used as a potential biomaterial for guided bone regeneration in periodontal applications.Article Citation - WoS: 43Citation - Scopus: 47Bioactive Diatomite and Poss Silica Cage Reinforced Chitosan/Na-carboxymethyl Cellulose Polyelectrolyte Scaffolds for Hard Tissue Regeneration(Elsevier, 2019) Tamburacı, Sedef; Kimna, Ceren; Tıhmınlıoğlu, FundaRecently, natural polymers are reinforced with silica particles for hard tissue engineering applications to induce bone regeneration. In this study, as two novel bioactive agents, effects of diatomite and polyhedral oligomeric silsesquioxanes (POSS) on chitosan (CS)/Na-carboxymethylcellulose (Na-CMC) polymer blend scaffolds are examined. In addition, the effect of silica reinforcements was compared with Si-substituted nano-hydroxyapatite (Si-Hap) particles. The morphology, physical and chemical structures of the scaffolds were characterized with SEM, liquid displacement, FT-IR, mechanical analysis, swelling and degradation studies. The particle size and the crystal structure of diatomite, POSS and Si-Hap particles were determined with DLS and XRD analyses. In vitro studies were performed to figure out the cytotoxicity, proliferation, ALP activity, osteocalcin production and biomineralization to demonstrate the promising use of natural silica particles in bone regeneration. Freeze-dried scaffolds showed 190-307 mu m pore size range and 61-70% porosity. Both inorganic reinforcements increased the mechanical strength, enhanced the water uptake capacity and fastened the degradation rate. The nanocomposite scaffolds did not show any cytotoxic effect and enhanced the surface mineralization in osteogenic medium. Thus, diatomite and POSS cage structures can be potential reinforcements for nanocomposite design in hard tissue engineering applications.Article Citation - WoS: 46Citation - Scopus: 53Chitosan-Hybrid Poss Nanocomposites for Bone Regeneration: the Effect of Poss Nanocage on Surface, Morphology, Structure and in Vitro Bioactivity(Elsevier, 2020) Tamburacı, Sedef; Tıhmınlıoğlu, FundaPOSS, regarded as the smallest silica particle, is widely used as nanofiller in polymer systems. POSS-based nanocomposites are deduced as novel materials having potency for biomedical applications owing to the enhanced biocompatibility and physicochemical characteristics. The aim of this work was to integrate the beneficial features of chitosan (CS) and OctaTMA-POSS nanoparticle to design nanocomposite for bone tissue regeneration. The nanocomposite scaffolds were fabricated by freeze-drying. The effects of POSS incorporation on morphology and structure of CS matrix were examined. Bioactivity and osteogenic effects of the POSS nanoparticles were investigated with cytocompatibility, cell proliferation, alkaline phosphatase activity, osteocalcin production and biomineralization assays. PUSS incorporation altered the surface morphology by increasing surface roughness. Nanocomposite scaffolds with 82-90% porosity exhibited an increase in compression modulus of scaffolds (78-107 kPa) compared to control CS group (56 kPa). Results indicated that CS-POSS scaffolds were found cytocompatible with 3T3, MG-63 and Saos-2 cell lines. POSS incorporation showed promising effects on osteoblast adhesion and proliferation as well as increasing ALP activity, octeocalcin secretion and biomineralization of cells. (C) 2019 Elsevier B.V. All rights reserved.Article Citation - WoS: 9Citation - Scopus: 10Decontamination of Seeds Destined for Edible Sprout Production From Listeria by Using Chitosan Coating With Synergetic Lysozyme-Nisin Mixture(Elsevier, 2020) Sözbilen, Gözde Seval; Yemenicioğlu, AhmetThis study aimed at decontamination of seeds destined for edible sprout production from Listeria using chitosan (CS) coatings incorporated with synergetic lysozyme-nisin (LYS-NIS) mixtures. Low molecular weight (LMW) CS coating showed the highest potency against Listeria innocua, followed by medium molecular weight (MMW) and high molecular weight (HMW) CSs. The LMW CS film with LYS-NIS also caused almost 1.5-fold greater log reduction (similar to 5 log) in initial L. innocua load of broth culture than MMW and HMW CS films with LYS-NIS within 6 days. Moreover, LMW CS coating with LYS-NIS reduced the initial Listeria loads of inoculated mung beans, lentils, and wheats by 3.3, 3.4 and 4.1 log, respectively. Antimicrobial coating did not affect seed germination rates considerably. The LYS-NIS addition increased yellowness and opacity of films, and caused limited changes in their mechanical and morphological properties. LMW CS coating with LYS-NIS reduces risk of listeriosis from sprouted seeds.Article Citation - WoS: 51Citation - Scopus: 60Biosilica Incorporated 3d Porous Scaffolds for Bone Tissue Engineering Applications(Elsevier Ltd., 2018) Tamburacı, Sedef; Tıhmınlıoğlu, FundaAs a natural and abundant silica mineral, diatomite particles (SiO2-nH2O) have been used in several areas such as filtration, photonics, sound and heat insulation, filler material and drug delivery due to its abundance, inexpensive cost, unique morphology and porous structure. But up to date, diatomite incorporated silica based scaffolds have not been used for bone tissue engineering applications. In the present study, the goal was to combine the useful biomaterial properties of both chitosan and diatomite as biocomposite organic/inorganic biomaterial for bone tissue engineering applications and optimize the silica content of the composites in order to obtain optimum morphological structure, high mechanical properties, enlarged surface area and enhanced cell proliferation. The effect of silica loading on the mechanical, morphological, chemical, and surface properties, wettability and biocompatibility of composite scaffolds were investigated. In addition, in vitro cytotoxicity and cellular activities including cell proliferation, ALP activity and biomineralization were investigated in order to determine biological activity of the composite scaffolds. Diatomite particles lead to enhancement in the water uptake capacity of scaffolds. Chitosan-silica composites exhibited 82–90% porosity. Wet chitosan-silica composite scaffolds exhibited higher compression moduli when compared to pure chitosan scaffold in the range of 67.3–90.1 kPa. Average pore size range of chitosan-diatomite composite scaffolds was obtained as 218-319 μm. In vitro results indicated that chitosan-diatomite composites did not show any cytotoxic effect on 3T3, MG-63 and Saos-2 cell lines. Scaffolds were found to be favorable for osteoblast proliferation. Diatomite incorporation showed promising effects on enhancing ALP activity as well as mineral formation on scaffold surface. Thus, the prepared scaffolds in this study can be considered prospective material for bone tissue engineering applications.Article Citation - WoS: 96Citation - Scopus: 113Hypericum Perforatum Incorporated Chitosan Films as Potential Bioactive Wound Dressing Material(Elsevier Ltd., 2017) Güneş, Seda; Tıhmınlıoğlu, FundaRecent studies in wound dressing applications offer new therapies and promote wound healing process. The aim of this study was to develop Hypericum perforatum (St John's Wort) oil incorporated chitosan films for wound dressing applications. H. perforatum oil as a potential therapeutic agent was encapsulated in chitosan film to achieve a better wound dressing material. Oil incorporated chitosan films were successfully prepared by solvent casting method in different oil concentrations (0.25–1.5%v/v). Water vapor permeability (WVP), mechanical test, swelling behavior and surface hydrophobicity were performed in order to characterize the prepared films. Antimicrobial test was performed by disc diffusion method and the growth inhibition effects of the films including different amount of H. perforatum oil were investigated on Escherichia coli and Staphylococcus aureus. WVP increased with oil incorporation and the highest value was obtained for 0.25% oil concentration.The highest strain value was obtained in 0.25% oil content films although tensile stress decreased with increasing oil content. H. perforatum oil incorporated films had antimicrobial effect on both microorganisms. Chitosan based films had no cytotoxic effects on NIH3T3fibroblast cells and provided a good surface for cell attachment and proliferation. The results showed that the H. perforatum incorporated chitosan films seems to be a potential and novel biomaterial for wound healing applications.Article Citation - WoS: 16Citation - Scopus: 18Cytotoxic and Cytostatic Side Effects of Chitosan Nanoparticles as a Non-Viral Gene Carrier(Elsevier Ltd., 2016) Bor, Gizem; Mytych, Jennifer; Zebrowski, Jacek; Wnuk, Maciej; Şanlı Mohamed, GülşahAlthough chitosan nanoparticles (CNs) became a promising tool for several biological and medical applications owing to their inherent biocompatibility and biodegradability features, studies regarding their effects on cytotoxic and cytostatic properties still remain insufficient. Therefore, in the present study, we decided to perform comprehensive analysis of the interactions between CNs–pKindling-Red-Mito (pDNA) and different cell line models derived from blood system and human solid tissues cancers. The resulting CNs-pDNA was investigated in terms of their cellular uptake, transfection efficiency, and physico-chemical, cytotoxic and cytostatic properties. The nanoparticles showed high encapsulation efficiency and physical stability for various formulations even after two days time period. Moreover, high gene expression levels were observed after 96 h of transfection. CNs-pDNA treatment, despite the absence of oxidative stress induction, caused cell cycle arrest in G0/G1 phase and as a consequence led to premature senescence which turned out to be both p21-dependent and p21-independent. Also, observed DNMT2 upregulation may suggest the activation of different pathways protecting from the results of CNs-mediated stress. In conclusion, treatment of different cell lines with CNs-pDNA showed that their biocompatibility was limited and the effects were cell type-dependent.