Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 1Citation - Scopus: 1Enhancing the Sustainable Protein Extraction Process From Black Cumin Seed Cake Press for Eco-Friendly Protein Supplements(Springer Science and Business Media Deutschland GmbH, 2025) Ammas, B.; Uzuner, Sibel; Uzuner, S.Finding new protein sources is essential to meet the global population’s needs. Nigella sativa (black cumin) seeds and the residual cake left after oil cold pressing contain valuable nutritional compounds, including proteins, phenolics, essential amino acids, and various bioactive compounds. In the literature, the structural characteristics and functional properties of proteins from black cumin seeds have been extensively studied. However, there are limited reports on the use of emerging extraction technologies for extracting soluble proteins from black cumin seed cake press (BCSCP). Therefore, two distinct extraction techniques—microwave (MW) and ultrasonication (US)—were assessed for their effectiveness in extracting soluble proteins from BCSCP. The aim of this study was to investigate the effects of various operational conditions on protein extraction yield and functional properties of proteins extracted from BCSCP. The maximum protein yield (27.04%) was achieved using a 10% solid/liquid ratio (SLR) at 33 °C for a 30-min extraction time with the US extraction technique. After ultrafiltration, the protein yield increased to 33.83%, showcasing the predominance of sarcosine (17.18 g/100 g), glutamine (11.63 g/100 g), and proline (33.46 g/100 g) amino acids. The water-holding capacity (WHC), oil-holding capacity (OHC), foam capacity (FC), and foam stability (FS) of the BCSCP isolate were found to be 5.4 ± 0.3 g/g, 9.4 ± 0.0 g/g, 43.6 ± 6.4%, and 39.0 ± 4.7%, respectively. The study revealed that US is a suitable extraction process for obtaining soluble proteins from BCSCP with favorable functional properties. Further studies are needed to evaluate the bioavailability and health impacts of bioactive peptides in order to develop food applications. © The Author(s) 2024.Article Citation - WoS: 20Citation - Scopus: 24Preparation and Characterization of Sodium Dodecyl Sulfate Doped Polypyrrole Solid Phase Micro Extraction Fiber and Its Application To Endocrine Disruptor Pesticide Analysis(Elsevier Ltd., 2013) Korba, Korcan; Pelit, Levent; Okçu Pelit, Füsun; Özdokur, K. Volkan; Ertaş, Hasan; Eroğlu, Ahmet Emin; Ertaş, Fatma NilA robust in house solid-phase micro extraction (SPME) surface has been developed for the headspace (HS)-SPME determination of endocrine disruptor pesticides, namely, Chlorpyrifos, Penconazole, Procymidone, Bromopropylate and Lambda-Cyhalothrin in wine sample by using sodium dodecylsulfate doped polypyrrole SPME fiber. Pyrrole monomer was electrochemically polymerized on a stainless steel wire in laboratory conditions in virtue of diminishing the cost and enhancing the analyte retention on its surface to exert better selectivity and hence the developed polymerized surface could offer to analyst to exploit it as a fiber in headspace SPME analysis. The parameters, mainly, adsorption temperature and time, desorption temperature, stirring rate and salt amount were optimized to be as 70°C and 45min, 200°C, 600rpm and 10gL-1, respectively. Limit of detection was estimated in the range of 0.073-1.659ngmL-1 for the pesticides studied. The developed method was applied in to red wine sample with acceptable recovery values (92-107%) which were obtained for these selected pesticides.Article Citation - WoS: 18Citation - Scopus: 22Fucoxanthin Content of Cylindrotheca Closterium and Its Oxidative Stress Mediated Enhancement(Central Fisheries Research Inst, 2016) Erdoğan, Ayşegül; Demirel, Zeliha; Conk Dalay, Meltem; Eroğlu, Ahmet EminProduction of fucoxanthin by diatoms has become an alternative research area due to their low cost, convenience and diversity. The fucoxanthin content of Cylindrotheca closterium and its enhancement by altering the cultivation conditions via oxidative stress were investigated in this study. For this purpose, the extraction parameters were optimized and the highest fucoxanthin concentration (6.58 mg g-1) was achieved within 15.0 minutes at 40 °C. Then, this yield reached to 10.15 mg g-1 in the presence of NaOCl and Fe2+. It is worth noting that, this is the first time that the effect of oxidative stress on fucoxanthin production in diatom has been studied according to our knowledge. Therefore, the results of this study and the discussion about the mechanisms can be a reference for the enrichment of fucoxanthin from other diatoms.
