Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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Subject: search.filters.subject.GenotypingWoS Q: search.filters.wosQuartile.Q4

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  • Article
    The Effectiveness of Genetic Markers and the Role of Environmental Factors in Hip Dysplasia and Osteochondritis Dissecans of the Shoulder in German Shepherd, Labrador Retriever, and German Wirehaired Pointer (Deutsch Drahthaar) Dogs
    (Elsevier, 2025) Ardicli, Sena; Yigitgor, Pelin; Ozen, Dogukan; Babayev, Huseyn; Bozkurt, Berkay; Senturk, Nursen; Intas, Deniz Seyrek
    Canine Hip Dysplasia (CHD) is the most frequently diagnosed orthopedic condition in dogs. Similar to CHD, osteochondritis dissecans (OCD) of the shoulder is a developmental disorder in dogs that significantly impacts animal welfare. As polygenic genetic disorders, they exhibit a complex mode of inheritance. Although there are numerous clinical studies, there is insufficient information about the genetic basis of these disorders. Therefore, this study aimed to assess the relationship of the prognostic genetic test markers with CHD and OCD in German Shepherd, Labrador Retriever, and German Wirehaired Pointer dogs. We evaluated the efficiency of five SNP markers from the prognostic genetic test for CHD (the Dysgen test) based on available GWAS data in German Shepherd, Labrador Retriever, and German Wirehaired Pointer dogs. Radiographs were captured and assessed according to the official FCI scale for hip dysplasia. In German Wirehaired Pointers, shoulder X-ray evaluations were also performed. We used custom FRET-based primer probes in Real-time PCR and Sanger sequencing for genotyping and tested the evaluation using multiple logistic regression procedures. German shepherds emerged as the most vulnerable to CHD (P < 0.001). In the final logistic model, females are expected to have a 3.54 times higher likelihood of experiencing CHD compared to males (P < 0.05). SNP BICF2G630558239 demonstrated a notable association with CHD, indicating that the GG genotype poses a risk. This SNP is situated in the intronic region of the KIF26B gene, a member of the kinesin superfamily implicated in evolutionarily conserved roles in embryogenesis. We did not observe any association between shoulder OCD-related arthrosis and the SNPs studied. These results may contribute to understanding CHD by identifying genotypes associated with epidemiological risk, prompting the need to conduct more thorough investigations.
  • Article
    Citation - WoS: 2
    Citation - Scopus: 4
    Genotyping of Various Arcobacter Species Isolated From Domestic Geese by Randomly Amplified Polymorphic Dna (rapd) Analysis
    (M. & H. Schaper, 2008) Atabay, Halil İbrahim; Ünver, Ahmet; Otlu, Salih; Kalaycıoğlu, Atila Taner
    The present study was undertaken to genotype Arcobacter (A.) butzleri, A. cryaerophilus and A. skirrowii isolates from domestic geese from three different flocks in Turkey. Fifteen Arcobacter isolates were analysed to determine the RAPD profiles based on the amplified DNA fragment patterns using a universal primer for genotyping. 7 A. cryaerophilus, 2 A. butzleri and 6 A. skirrowii isolates produced 6, 2 and 3 distinct profiles, respectively. The isolates of the same patterns originated from the same flocks. The findings of the present study may support previous reports of the existence of a large degree of heterogeneity among Arcobacter isolates. Observation of such levels of genetic diversity may suggest that there are multiple contamination sources in the environment and/or the determined genotypes may have undergone genetic rearrangements. This first report of genotyping of various Arcobacter species isolated from healthy geese is expected to improve the understanding of the ecology and epidemiology of this emerging pathogen.