Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7148

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  • Article
    Citation - Scopus: 5
    Ultrasound Assisted Extraction for the Recovery of Phenolic Compounds From Waste Hazelnut Shell
    (Yildiz Technical University, 2020) Dal,O.; Şengün,D.; Özşen,A.Y.
    Hazelnut shell is the primary byproduct of hazelnut industry which has the potential source of antioxidants, and phenolics with interest of pharmaceutical, food, and cosmetic industries. The main goal of this study is to determine effects of extraction method, extraction time, solvent type, solid to liquid ratio, and particle size on extraction yield, antioxidant capacity, and total phenolic content of waste hazelnut shell. The highest extraction yield was found as 15.4% by using methanol as solvent, in combined extraction for 16 h total extraction time. As for the best antioxidant capacity, 0.0508 mg TE mL-1 was observed by using methanol as a solvent in ultrasonic extraction, whereas the highest phenolic content was found as 0.188 mg GAE mL-1 by Soxhlet extraction with acetone for 8 h. After extraction of hazelnut shell waste, major components were found as oleic and palmitic acids for all solvent types according to GC-MS results. © Yildiz Technical University, Environmental Engineering Department. All rights reserved.
  • Article
    Citation - WoS: 3
    Citation - Scopus: 3
    Comprehensive Analysis of Lutein and Loroxanthin in <i>scenedesmus Obliquus</I>: From Quantification To Isolation
    (Mdpi, 2024) Erdogan, Aysegul; Karatas, Ayca Busra; Demir, Dilan; Demirel, Zeliha; Akturk, Merve; Copur, Oyku; Conk-Dalay, Meltem
    Carotenoids are hydrophobic pigments produced exclusively by plants, fungi, and specific microbes. Microalgae are well suited for the production of valuable carotenoids due to their rapid growth, efficient isoprenoid production pathway, and ability to store these compounds within their cells. The possible markets for bio-products range from feed additives in aquaculture and agriculture to pharmaceutical uses. The production of carotenoids in microalgae is affected by several environmental conditions, which can be utilized to enhance productivity. The current study focused on optimizing the extraction parameters (time, temperature, and extraction number) to maximize the yield of carotenoids. Additionally, the impact of various nitrogen sources (ammonia, nitrate, nitrite, and urea) on the production of lutein and loroxanthin in Scenedesmus obliquus was examined. To isolate the carotenoids, 0.20 g of biomass was added to 0.20 g of CaCO3 and 10.0 mL of ethanol solution containing 0.01% (w/v) pyrogallol. Subsequently, the extraction was performed using an ultrasonic bath for a duration of 10 min at a temperature of 30 degrees C. This was followed by a four-hour saponification process using a 10% methanolic KOH solution. The concentration of lutein and loroxanthin was measured using HPLC-DAD at 446 nm, with a flow rate of 1.0 mL/min using a Waters YMC C30 Carotenoid column (4.6 x 250 mm, 5 mu m). The confirmation of carotenoids after their isolation using preparative chromatography was achieved using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with an atmospheric pressure chemical ionization (APCI) probe and UV-vis spectroscopy. In summary, S. obliquus shows significant promise for the large-scale extraction of lutein and loroxanthin. The findings of this study provide strong support for the application of this technology to other species.