Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Investigation of Probiotic Properties of Cold Brewed and Fermented Cold Coffee Beverage(01. Izmir Institute of Technology, 2023) Semiz, Şevval; Baysal, Ayşe HandanThe goal of the project was to produce a functional probiotic fermented cold brewed coffee that is perapered with medium roasted, grinded Colombian Arabica beans with patented probiotic yeast Saccharomyces boulardii that can survive in human gastrointestinal conditions. With pasteurization of the product, safe fermentation environment and long shelf life was achieved. Coffee brew samples were prepared with different techniques to understand the effects of fermentation and glucose fortification to fermentation media on coffee chemistry, microbiota and sensory characteristic. One of the three coffee brews was only pasteurized (P), one of them fermented (F) and the other was fermented with glucose (FG). The cold brewing and fermentation were performed respectively at 22 °C and 30 °C for 24 hours. The brewed samples were pasteurized before fermentation at 65 °C for 30 minutes. Each sample was examined during their 120 days shelf life (at +4 °C). Although the probiotic yeast enabled fermentation in coffee, it did not survive after the first week. Pasteurization was successful, so pathogen growth was not detected in any of the samples during their shelf life. While FG was evaluated as undrinkable, the other samples gave positive results in terms of sensory. Caffeine, chlorogenic acid, and ethanol levels were significantly altered during shelf life. This is the first study in which chemical, microbiological and sensory analyzes of cold coffee that was brewed via cold immersion method, pasteurized and fermented with patented S. boulardii yeast during shelf-life were conducted, and will lead to new studies in this field.Master Thesis Dynamics and Bioinformatis of Microbial Spoilage Ecology of Kefir(Izmir Institute of Technology, 2022) Memon, Ayşe; Baysal, Ayşe Handan; Sezgin, EfeIn this study, it was aimed to characterize the microbiological properties, bacterial composition, and microbial stability of 5 different commercial milk kefir beverage products during refrigerated storage. In order to determine the microbiological load and compositions, total mesophilic aerobic bacteria, yeast and molds, lactic acid bacteria, Lactobacilli, Lactococci, total coliforms, and E.coli were investigated by cultural conventional analysis for milk kefir beverages. According to the obtained data, microbiological and hygienic characteristics of the samples were found acceptable. The bacterial load of the kefir beverage samples ranged between 7.086 and 8.794 log10 cfu.ml-1 for viable total aerobic mesophilic bacteria (TAMB), 6.792 and 8.382 og10 cfu.ml-1 for lactic bacteria (LAB), <10 and 6.322 log10 cfu.ml-1for Lactobacillus, 5.857 and 8.146 log10 cfu.ml-1 Lactococcus, 5.176 and 7.218 log10 cfu.ml-1 for yeasts, negative for molds, coliform bacteria and negative for E. coli. Principal component analysis (PCA) of the compounds separated the kefir beverages according to the storage time and kefir brands. Strong relationship were found between storage time and PC1 and between kefir brands and PC2. To date, information on microbial properties, bacterial composition, and constancy of commercial kefir is scant, and to the best of our knowledge, this is the first research to contribute information on kefir beverages in microbial properties, bacterial composition, and their stability during refrigerated storage by evaluating Fourier Transform Infrared Spectroscopy (FTIR) spectra analysis and Bioinformatics besides cultural conventional analysis.