Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
Browse
2 results
Search Results
Master Thesis Optimization of a Liver Organ on Chip System for the Investigation of Breast Cancer Cell Invasion(01. Izmir Institute of Technology, 2023) Tosunoğlu, Perge Bilgesu; Yalçın Özuysal, ÖzdenThere are many challenges in creating an accurate and physiologically relevant three-dimensional (3D) model of the liver such as finding the suitable liver extracellular matrix (ECM) components and cell types for the development of cell-laden liver-on-a-chip systems. In recent years, precision tissue slice-based methods are used to reduce the problems caused by the cell-laden liver-on-a-chip system complexity. However, these methods require sophisticated tools which are not easily accessible. This study focuses on the development of a 3D liver model using simple and cost-effective methods. To achieve this, we aimed to optimize liver tissue size, maintenance and culture medium, scaffold gel, and viability assay. The needle method was found to be the easiest, most efficient, and cheap method for tissue processing as the equipment used was easily accessible and evaded enzymatic steps. Moreover, consistent samples that were all similar and in the desired size were easy to obtain. In addition to these, functionality and viability were analysed for 72 hours to assess the physiological state of the liver after tissue processing. Based on these findings a novel liver-on-a-chip system was successfully developed and as the next step, the invasion of the MDA-MB-231 breast cancer cell line towards the liver was investigated with and without the presence of C-X-C chemokine receptor type 4 (CXCR4) antagonists AMD3100 and AMD3465. In conclusion, this study demonstrated the development of a novel 3D model for the liver and provided a platform for studying breast cancer invasion with its potential implications for cancer therapy research.Master Thesis Plant-On Devices for Sed Screening(Izmir Institute of Technology, 2019) Yetgin, Ali; Pesen Okvur, Devrim; Canlı, KeremThanks to their roots, plants take the required molecules from the soil, therefore their research is important. The fact that absorption takes place from the root hair makes the observations necessary. The inadequacy of traditional methods requires the development of new methods to ensure their observations, especially during root studies. The fact that chip systems have the features that can overcome the problems has caused plant researches by using this method. The fact that environmental factors can be imitated in chip systems paves the way for abiotic stresses to work. Although abiotic stresses have been studied extensively on plants, seed germination and subsequent root extension and root hair formation have not been studied in detail except for the model organism (Arabidopsis thaliana). The use of chip system for plant studies of scientists have begun the 21st century is also reveals the need to do more work in this area. Researchers show that they are excited about the advantages they bring to the system and that necessary studies will be carried out in other side branches. It is thought that more research will be done by using chip systems after a short time. By using chip systems, a suitable environment for seed growth can be provided and environmental conditions can be simulated. By creating the desired controlled environment, it is possible to create a system which results in less time and less cost in order to replace the inconvenient and expensive method.