Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Cloning of Polysome-Associated Small Rnas in Drosophila Melanogaster Embryos(Izmir Institute of Technology, 2009) Yiğit, Hatice; Akgül, Bünyamin; Akgül, Bünyamin; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyGenome-encoded regulatory small RNAs are classified into 3 groups; microRNAs (miRNAs), endogeneous small interfering RNAs (endo siRNAs) and piwi interacting RNAs (piRNAs). miRNAs, 17-21 nucleotide in size, are involved in posttranscriptional gene regulation via precise or imprecise base pairing with target mRNAs resulting in either target mRNA degradation or translational inhibition. Endo siRNAs ,on the other hand, may function transposon regulation but their precise regulatory function and mechanism have not been elucidated yet. piRNAs are mainly involved in transposon silencing in spermatogenesis. Despite their discovery, biological roles and modes of functions of small RNAs remain to be elucidated. The aim of this thesis was to identify polysome-associated small RNAs in Drosophila melanogaster embryos by deep sequencing and investigate their role in translational regulation. Deep sequencing and microarray results determined stage and fraction specific distribution of genome encoded small RNAs. Surprisingly, the results implied that mRNAs may be posttranscriptionally regulated by antisense transcripts in polysome.Master Thesis Genomic Profiling of Micrornas Regulating Translation in Drosophila Melanogaster Embryos(Izmir Institute of Technology, 2008) Tüncel, Özge; Akgül, Bünyamin; Tüncel, Özge; Akgül, Bünyamin; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyAmong the small RNAs, microRNAs are a particular class of 21 to 23 nucleotide RNAs that negatively regulate translation and play a pivotal role in posttranscriptional gene expression. microRNAs are found in many phyla that control such diverse events as metabolism, cell fate, cell death and development.The aim of this study is to investigate molecular mechanism of miRNAmediated translational regulation by profiling developmentally important microRNAs according to their translational status and to identify new microRNAs that have roles in translational regulation during the embryogenesis of Drosophila. Following RNA purification from different embryonal stages the fractionated RNAs were analyzed by microRNA microarray. Preliminary results show that 9 miRNAs were expressed in both stages whereas 60 miRNAs were accumulated in RNA fractions of 8 hour embryos.Also there are 2 miRNAs in all fractions of both stages in Drosophila embryos. It can be concluded that most of them were expressed in late embryonal development and there does not appear to be a switch in microRNA profiles in fractions for different stages of embryos. The preliminary results suggest that microRNAs may suppress protein synthesis at pre-initiation and initiation phases based on the microarray data.Further studies are required to solidify the preliminary findings.