Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Preparation of Drug Loaded Albumin Nanoparticles in Water / Ionic Liquids Microemulsion Systems(Izmir Institute of Technology, 2021) Yıldırım, Barış; Akdoğan, YaşarNanoparticles (NPs) have been used in various applications such as biotechnology, nanomedicine, and drug delivery systems. Many nanoparticle drug delivery systems have been promoted for cancer treatment, and numerous materials have been investigated to use as drug delivery agents to enhance the therapeutic efficiency and safety of anticancer drugs. Albumin is a natural biopolymer and the most abundant protein in blood plasma. Due to its versatile binding capacity of widespread therapeutical drugs, albumin becomes an ideal material to obtain nanoparticles. In this study, the ionic liquid (IL) based emulsification methods were investigated. Instead of classical toxic and volatile solvents, using ILs in microemulsions, environment-friendly media were received to synthesize bovine serum albumin (BSA) NPs. In order to obtain BSA NPs, high-speed homogenizer processing was applied by following crosslinker addition. The IL microemulsions are a thermodynamically stable colloidal dispersion containing spherical droplets (W/IL or IL/W) in submicron sizes that act as nanoreactors for NP formation. Chlorambucil (CHL) was used as a model drug to investigate drug loading and releasing kinetics of BSA NPs as a drug delivery candidate. Results showed that chlorambucil loading capacities and release kinetics depended on the synthesized medium such as anion-type of ILs and surfactants. CHL loaded to the BSA NPs synthesized in hydrophilic IL BmimBF4 in relatively higher amounts and released in the same trend. In addition, the cell viability effect of CHL-loaded BSA NPs synthesized in different types of ILs were investigated. The CHL-loaded BSA NPs synthesized in BmimOTf and BmimPF6 reduced the cancer cell viability more than the used same dose of free CHL.Master Thesis Preparation of Albumin Nanoparticles Using an Ionic Liquid Based Microemulsion-Like Method(Izmir Institute of Technology, 2018) Demirkurt, Begüm; Akdoğan, Yaşar; Adem, UmutRich drug transportation ability of serum albumin protein has inspired scientists to obtain drug nanocarriers from albumin. In the literature, different methods have been developed to prepare albumin nanoparticles and their drug delivery properties have been studied. Here, this study aims to obtain albumin nanoparticles for a first time using ionic liquid (IL) included systems. Goal of this project is using imidazolium based ionic liquids (green solvent) to prepare albumin nanoparticles as alternative solvents for the commonly used organic solvents. The use of volatile, toxic and flammable organic solvents in the albumin nanoparticle production has various negative effects on both human health and environment. Ionic liquids as non-flammable, non-volatile and non-toxic solvent candidates have attracted considerable attention in recent years both in the literature and in industry. Their ability to solve different types of solutes, designability, special mixing ability with water in IL/water binary systems and environmentally friendly properties cause ILs to overtake traditional organic solvents. This thesis study proposed a novel and environmentally friendly microemulsionlike method for producing albumin nanoparticles in IL/water binary systems. Various experimental parameters such as pH effects, albumin concentrations, water amount, surfactant effects, glutaraldehyde effects, homogenizer effects, etc. were investigated to obtain uniform albumin nanoparticles. As a result, we achieved to synthesize uniformly distributed 200 nm average size albumin nanoparticles at pH 9.0 using 1.5% (w/w) of bovine serum albumin (BSA) in 1-butyl-3-methylimidazolium tetrafluoroborate using TX-100/n-butanol surfactant mixture.