Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Co-ni Separation In Ix-sx Circuit(2023) Polat, Mehmet; Polat, Mehmet; 03.02. Department of Chemical Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyEti Gübre stands out as the sole facility capable of reclaiming multiple metals from chalcopyrite flotation tails in Turkey. While the overall performance of the process has been deemed satisfactory, the cobalt and nickel products have yet to meet commodity standards. The objective of this project is to ascertain the feasibility of using a series of hydrometallurgical techniques, namely, solvent extraction and ion exchange resin technologies, for attaining premium-quality cobalt and nickel products from a compound comprising cobalt, nickel, and other assorted elements. Extracting cobalt and nickel selectively from impurities, reaching over 95% recovery in a solution containing various elements and steep concentration differences, presents a considerable challenge. Moreover, accomplishing this within an already operational process adds another layer of complexity, demanding alignment with the existing process's stability. The strategy involved selectively separating and refining cobalt and nickel from the sulfate solution through solvent extraction (SX) methods and employing the Ion Exchange (IX) method for further purification.Master Thesis Identification and Characaterization of Nickel Tolerance Genes in Beta Maritima(Izmir Institute of Technology, 2009) Bozdağ, Gönensin Ozan; Karakaya, Hüseyin Çağlar; Karakaya, Hüseyin Çağlar; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of TechnologyNickel is an essential micronutrient for plant growth. However, an excess amount of nickel is toxic for plants and animals. The Beta maritima plant which is known crop as salt and manganese tolerant nature. In this study, we aimed to identify nickel detoxification metabolism genes of Beta maritima by screening plant.s overexpressed cDNA library in yeast cells. After initial screening, three transformants were isolated that were able to grow in the presence of toxic nickel concentration. After the sequence analyses, three genes were named as NIC3, NIC6 and NIC8. We tested the growth rates of the yeast cells those were overexpressing cDNAs in solid mediums and showed similar nickel detoxification patterns. Then intracellular nickel concentrations were measured to see whether mentioned cDNAs pump nickel out of the cell or not. We also analysed the gene expression levels of NIC3 and NIC6 genes after appllying nickel stress to the plants to see the relations between nickel treatment and transcription changes of these two genes. Our data suggest that NIC3, NIC6 and NIC8 genes, besides their other functions, confer yeast cells nickel tolerance and may play roles in heavy metal detoxification for plant cells.