Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
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Master Thesis Green Synthesis of Nanostructured Bioactive Glass for Dental Applications(01. Izmir Institute of Technology, 2023) Tüncer, Melisa; Öksel Karakuş, Ceyda; Yücesoy, Deniz Tanıl; Yücesoy, Deniz Tanıl; Öksel Karakuş, Ceyda; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyBioactive glass is a biomaterial commonly used in dental care products and bone tissue engineering applications due to its biocompatibility, bone-forming ability, and remineralization capability. Bioactive glasses form a hydroxyapatite-like layer on dentinal tubules by releasing calcium and phosphorus ions after interaction with saliva. Bioactive 45S5 glass traditionally synthesized by wet chemical methods which require high-temperature heating and the use of a strong acid catalyst, bringing into question of the possibility of introducing toxic acid residues into the final product. Therefore, there is a need to develop environmental-friendly bioactive glass synthesis methods or to modify existing ones in a way to uplift their environmental friendliness. To satisfy this need, we greenized the traditional sol-gel method by replacing the acid catalyst with an environment-friendly alternative and successfully used it for the synthesis of nanostructured 45S5 bioactive glass. First, physicochemical characterization of the synthesized bioactive glasses was performed. Then, the apatite formation capability of bioglasses were investigated in saliva. Next, the mineralization kinetics of bioglasses were tested in Ca/P buffer. In vitro toxicity tests were performed to assess the cytotoxic potential of the synthesized bioactive glass. All analyses were repeated for the traditional synthesis method for comparison purposes. The results confirmed that green synthesis is more advantageous in terms of bioactivity and functionality required for dental applications. Increasing the safety and functionality of bioglass at the same time during the production phase has critical importance for ensuring the sustainability of current applications as well as creating new uses in the biomedical fieldMaster Thesis Optimizing the Dispersion of Ceramic Nanoparticles and Assessing the Role of Aggregation in Mediating Biological Activity(Izmir Institute of Technology, 2022) Öksel Karakuş, Ceyda; Öksel Karakuş, Ceyda; Öksel Karakuş, Ceyda; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe aim of this study is to optimize the sample preparation protocol for dispersing powder-form ceramic nanoparticles (CeNPs) in liquid media by leveraging the power of the design of experiment approach to narrow down potential causes of aggregation and to investigate the subsequent effect of CeNP aggregation on the biological activity of SaOS-2 human osteosarcoma cells. Dispersion of the nanopowders is one of the problems in the nanotechnology field because of the tendency of the nanoparticles for aggregation. Although there are existing dispersion protocols, they offer a one-size-fits-all approach overseeing the unique physicochemical properties of the different nanomaterials. In this study, optimization of the sample preparation protocol for two CeNPs was assessed via the investigation of the most contributing parameters and their synergetic effect through measurements of Z-average and zeta potential. Evaluation of these parameters allowed the development of two different models for each nanomaterial, predicting Z-average and zeta potential for given parameter sets. Through these models, two different sample sets were selected to evaluate the effect of aggregation on the SaOS-2 cell line. Outcomes show that the concentration of nanomaterial, pH, and the presence of additive molecule are three main parameters that affect dispersion stability. It was seen that these parameters can be included in a design to develop an efficient model to predict Z-average and zeta potential for investigated nanomaterials. Moreover, cell viability tests show that there is no significant difference between untreated and nanomaterial-treated cells. The findings promise that tailor-made and reliable dispersion protocols for different nanopowders can be developed via design of experiment.